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Ectopic activation of the rice NLR heteropair RGA4/RGA5 confers resistance to bacterial blight and bacterial leaf streak diseases.
Plant J 2016; 88(1):43-55PJ

Abstract

Bacterial blight (BB) and bacterial leaf streak (BLS) are important diseases in Oryza sativa caused by Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), respectively. In both bacteria, transcription activator-like (TAL) effectors are major virulence determinants that act by transactivating host genes downstream of effector-binding elements (EBEs) bound in a sequence-specific manner. Resistance to Xoo is mostly related to the action of TAL effectors, either by polymorphisms that prevent the induction of susceptibility (S) genes or by executor (R) genes with EBEs embedded in their promoter, and that induce cell death and resistance. For Xoc, no resistance sources are known in rice. Here, we investigated whether the recognition of effectors by nucleotide binding and leucine-rich repeat domain immune receptors (NLRs), the most widespread resistance mechanism in plants, is also able to stop BB and BLS. In one instance, transgenic rice lines harboring the AVR1-CO39 effector gene from the rice blast fungus Magnaporthe oryzae, under the control of an inducible promoter, were challenged with transgenic Xoo and Xoc strains carrying a TAL effector designed to transactivate the inducible promoter. This induced AVR1-CO39 expression and triggered BB and BLS resistance when the corresponding Pi-CO39 resistance locus was present. In a second example, the transactivation of an auto-active NLR by Xoo-delivered designer TAL effectors resulted in BB resistance, demonstrating that NLR-triggered immune responses efficiently control Xoo. This forms the foundation for future BB and BLS disease control strategies, whereupon endogenous TAL effectors will target synthetic promoter regions of Avr or NLR executor genes.

Authors+Show Affiliations

UMR Interactions Plantes-Microorganismes-Environnement, IRD-Cirad-UM, Montpellier, France.INRA, Biology and Genetics of Plant-Pathogen Interactions Laboratory, Montpellier, France. CIRAD, Biology and Genetics of Plant-Pathogen Interactions Laboratory, Montpellier, France. SUPAGRO, Biology and Genetics of Plant-Pathogen Interactions Laboratory, Montpellier, France.INRA, Biology and Genetics of Plant-Pathogen Interactions Laboratory, Montpellier, France. CIRAD, Biology and Genetics of Plant-Pathogen Interactions Laboratory, Montpellier, France. SUPAGRO, Biology and Genetics of Plant-Pathogen Interactions Laboratory, Montpellier, France.INRA, Biology and Genetics of Plant-Pathogen Interactions Laboratory, Montpellier, France. CIRAD, Biology and Genetics of Plant-Pathogen Interactions Laboratory, Montpellier, France. SUPAGRO, Biology and Genetics of Plant-Pathogen Interactions Laboratory, Montpellier, France.UMR Interactions Plantes-Microorganismes-Environnement, IRD-Cirad-UM, Montpellier, France.Department of Genetics, Martin Luther University Halle-Wittenberg, Halle (Saale), 06099, Germany.UMR Interactions Plantes-Microorganismes-Environnement, IRD-Cirad-UM, Montpellier, France.UMR Interactions Plantes-Microorganismes-Environnement, IRD-Cirad-UM, Montpellier, France. boris.szurek@ird.fr.INRA, Biology and Genetics of Plant-Pathogen Interactions Laboratory, Montpellier, France. thomas.kroj@supagro.inra.fr. CIRAD, Biology and Genetics of Plant-Pathogen Interactions Laboratory, Montpellier, France. thomas.kroj@supagro.inra.fr. SUPAGRO, Biology and Genetics of Plant-Pathogen Interactions Laboratory, Montpellier, France. thomas.kroj@supagro.inra.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27289079

Citation

Hutin, Mathilde, et al. "Ectopic Activation of the Rice NLR Heteropair RGA4/RGA5 Confers Resistance to Bacterial Blight and Bacterial Leaf Streak Diseases." The Plant Journal : for Cell and Molecular Biology, vol. 88, no. 1, 2016, pp. 43-55.
Hutin M, Césari S, Chalvon V, et al. Ectopic activation of the rice NLR heteropair RGA4/RGA5 confers resistance to bacterial blight and bacterial leaf streak diseases. Plant J. 2016;88(1):43-55.
Hutin, M., Césari, S., Chalvon, V., Michel, C., Tran, T. T., Boch, J., ... Kroj, T. (2016). Ectopic activation of the rice NLR heteropair RGA4/RGA5 confers resistance to bacterial blight and bacterial leaf streak diseases. The Plant Journal : for Cell and Molecular Biology, 88(1), pp. 43-55. doi:10.1111/tpj.13231.
Hutin M, et al. Ectopic Activation of the Rice NLR Heteropair RGA4/RGA5 Confers Resistance to Bacterial Blight and Bacterial Leaf Streak Diseases. Plant J. 2016;88(1):43-55. PubMed PMID: 27289079.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ectopic activation of the rice NLR heteropair RGA4/RGA5 confers resistance to bacterial blight and bacterial leaf streak diseases. AU - Hutin,Mathilde, AU - Césari,Stella, AU - Chalvon,Véronique, AU - Michel,Corinne, AU - Tran,Tuan Tu, AU - Boch,Jens, AU - Koebnik,Ralf, AU - Szurek,Boris, AU - Kroj,Thomas, Y1 - 2016/08/18/ PY - 2016/02/17/received PY - 2016/06/01/revised PY - 2016/06/03/accepted PY - 2016/10/25/pubmed PY - 2017/10/21/medline PY - 2016/6/12/entrez SP - 43 EP - 55 JF - The Plant journal : for cell and molecular biology JO - Plant J. VL - 88 IS - 1 N2 - Bacterial blight (BB) and bacterial leaf streak (BLS) are important diseases in Oryza sativa caused by Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), respectively. In both bacteria, transcription activator-like (TAL) effectors are major virulence determinants that act by transactivating host genes downstream of effector-binding elements (EBEs) bound in a sequence-specific manner. Resistance to Xoo is mostly related to the action of TAL effectors, either by polymorphisms that prevent the induction of susceptibility (S) genes or by executor (R) genes with EBEs embedded in their promoter, and that induce cell death and resistance. For Xoc, no resistance sources are known in rice. Here, we investigated whether the recognition of effectors by nucleotide binding and leucine-rich repeat domain immune receptors (NLRs), the most widespread resistance mechanism in plants, is also able to stop BB and BLS. In one instance, transgenic rice lines harboring the AVR1-CO39 effector gene from the rice blast fungus Magnaporthe oryzae, under the control of an inducible promoter, were challenged with transgenic Xoo and Xoc strains carrying a TAL effector designed to transactivate the inducible promoter. This induced AVR1-CO39 expression and triggered BB and BLS resistance when the corresponding Pi-CO39 resistance locus was present. In a second example, the transactivation of an auto-active NLR by Xoo-delivered designer TAL effectors resulted in BB resistance, demonstrating that NLR-triggered immune responses efficiently control Xoo. This forms the foundation for future BB and BLS disease control strategies, whereupon endogenous TAL effectors will target synthetic promoter regions of Avr or NLR executor genes. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/27289079/Ectopic_activation_of_the_rice_NLR_heteropair_RGA4/RGA5_confers_resistance_to_bacterial_blight_and_bacterial_leaf_streak_diseases_ L2 - https://doi.org/10.1111/tpj.13231 DB - PRIME DP - Unbound Medicine ER -