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Molecular characterization and expression of Na+/K+-ATPase α1 isoforms in the European sea bass Dicentrarchus labrax osmoregulatory tissues following salinity transfer.
Fish Physiol Biochem 2016; 42(6):1647-1664FP

Abstract

The Na+/K+-ATPase (NKA) is considered as the main pump involved in active ion transport. In the European sea bass, Dicentrarchus labrax, we found two genes encoding for the alpha 1 subunit isoforms (NKA α1a and NKA α1b). NKA α1a and NKA α1b isoform amino acid (aa) sequences were compared through phylogeny and regarding key functional motifs between salmonids and other acanthomorph species. Analysis of aa sequences of both isoforms revealed a high degree of conservation across teleosts. The expression pattern of both nka α1a and nka α1b was measured in the gill, kidney and posterior intestine of fish in seawater (SW) and transferred to fresh water (FW) at different exposure times. Nka α1a was more expressed than nka α1b whatever the condition and the tissue analyzed. After long-term salinity acclimation (2.5 years) either in FW or SW, transcript levels of nka α1a were higher in the kidney followed by the posterior intestine and the gill. Compared to SW conditions, expression of nka α1a in FW was significantly increased or decreased, respectively, in gill and posterior intestine. In contrast, branchial nka α1b was significantly decreased in FW-acclimated fish. Short-term FW acclimation seems to rapidly increase nka α1a transcript levels in the kidney unlike in gill tissues where different gene expression levels are detected only after long-term acclimation.

Authors+Show Affiliations

Adaptation Ecophysiologique et Ontogenèse (AEO, UMR 9190 MARBEC), Université de Montpellier, Place Eugène Bataillon - CC092, 34095, Montpellier Cedex 5, France.Adaptation Ecophysiologique et Ontogenèse (AEO, UMR 9190 MARBEC), Université de Montpellier, Place Eugène Bataillon - CC092, 34095, Montpellier Cedex 5, France.Unité Biologie des Organismes et écosystèmes aquatiques (BOREA, UMR 7208), CNRS, IRD 207, Sorbonne Universités, Muséum national d'Histoire naturelle, Université Pierre et Marie Curie, Université de Caen Basse Normandie, CP32, 7 rue Cuvier, 75005, Paris, France.Adaptation Ecophysiologique et Ontogenèse (AEO, UMR 9190 MARBEC), Université de Montpellier, Place Eugène Bataillon - CC092, 34095, Montpellier Cedex 5, France.Adaptation Ecophysiologique et Ontogenèse (AEO, UMR 9190 MARBEC), Université de Montpellier, Place Eugène Bataillon - CC092, 34095, Montpellier Cedex 5, France.Adaptation Ecophysiologique et Ontogenèse (AEO, UMR 9190 MARBEC), Université de Montpellier, Place Eugène Bataillon - CC092, 34095, Montpellier Cedex 5, France. catherine.lorin@umontpellier.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27289588

Citation

Blondeau-Bidet, Eva, et al. "Molecular Characterization and Expression of Na+/K+-ATPase Α1 Isoforms in the European Sea Bass Dicentrarchus Labrax Osmoregulatory Tissues Following Salinity Transfer." Fish Physiology and Biochemistry, vol. 42, no. 6, 2016, pp. 1647-1664.
Blondeau-Bidet E, Bossus M, Maugars G, et al. Molecular characterization and expression of Na+/K+-ATPase α1 isoforms in the European sea bass Dicentrarchus labrax osmoregulatory tissues following salinity transfer. Fish Physiol Biochem. 2016;42(6):1647-1664.
Blondeau-Bidet, E., Bossus, M., Maugars, G., Farcy, E., Lignot, J. H., & Lorin-Nebel, C. (2016). Molecular characterization and expression of Na+/K+-ATPase α1 isoforms in the European sea bass Dicentrarchus labrax osmoregulatory tissues following salinity transfer. Fish Physiology and Biochemistry, 42(6), pp. 1647-1664.
Blondeau-Bidet E, et al. Molecular Characterization and Expression of Na+/K+-ATPase Α1 Isoforms in the European Sea Bass Dicentrarchus Labrax Osmoregulatory Tissues Following Salinity Transfer. Fish Physiol Biochem. 2016;42(6):1647-1664. PubMed PMID: 27289588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization and expression of Na+/K+-ATPase α1 isoforms in the European sea bass Dicentrarchus labrax osmoregulatory tissues following salinity transfer. AU - Blondeau-Bidet,Eva, AU - Bossus,Maryline, AU - Maugars,Gersende, AU - Farcy,Emilie, AU - Lignot,Jehan-Hervé, AU - Lorin-Nebel,Catherine, Y1 - 2016/06/11/ PY - 2016/02/05/received PY - 2016/06/01/accepted PY - 2016/6/13/pubmed PY - 2016/6/13/medline PY - 2016/6/13/entrez KW - Fish KW - Gene expression KW - Isoform KW - Na+/K+-ATPase KW - Osmoregulation KW - Salinity SP - 1647 EP - 1664 JF - Fish physiology and biochemistry JO - Fish Physiol. Biochem. VL - 42 IS - 6 N2 - The Na+/K+-ATPase (NKA) is considered as the main pump involved in active ion transport. In the European sea bass, Dicentrarchus labrax, we found two genes encoding for the alpha 1 subunit isoforms (NKA α1a and NKA α1b). NKA α1a and NKA α1b isoform amino acid (aa) sequences were compared through phylogeny and regarding key functional motifs between salmonids and other acanthomorph species. Analysis of aa sequences of both isoforms revealed a high degree of conservation across teleosts. The expression pattern of both nka α1a and nka α1b was measured in the gill, kidney and posterior intestine of fish in seawater (SW) and transferred to fresh water (FW) at different exposure times. Nka α1a was more expressed than nka α1b whatever the condition and the tissue analyzed. After long-term salinity acclimation (2.5 years) either in FW or SW, transcript levels of nka α1a were higher in the kidney followed by the posterior intestine and the gill. Compared to SW conditions, expression of nka α1a in FW was significantly increased or decreased, respectively, in gill and posterior intestine. In contrast, branchial nka α1b was significantly decreased in FW-acclimated fish. Short-term FW acclimation seems to rapidly increase nka α1a transcript levels in the kidney unlike in gill tissues where different gene expression levels are detected only after long-term acclimation. SN - 1573-5168 UR - https://www.unboundmedicine.com/medline/citation/27289588/Molecular_characterization_and_expression_of_Na+/K+_ATPase_α1_isoforms_in_the_European_sea_bass_Dicentrarchus_labrax_osmoregulatory_tissues_following_salinity_transfer_ L2 - https://dx.doi.org/10.1007/s10695-016-0247-x DB - PRIME DP - Unbound Medicine ER -