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New molecular markers and cytogenetic probes enable chromosome identification of wheat-Thinopyrum intermedium introgression lines for improving protein and gluten contents.
Planta. 2016 Oct; 244(4):865-76.P

Abstract

MAIN CONCLUSION

New molecular markers were developed for targeting Thinopyrum intermedium 1St#2 chromosome, and novel FISH probe representing the terminal repeats was produced for identification of Thinopyrum chromosomes. Thinopyrum intermedium has been used as a valuable resource for improving the disease resistance and yield potential of wheat. A wheat-Th. intermedium ssp. trichophorum chromosome 1St#2 substitution and translocation has displayed superior grain protein and wet gluten content. With the aim to develop a number of chromosome 1St#2 specific molecular and cytogenetic markers, a high throughput, low-cost specific-locus amplified fragment sequencing (SLAF-seq) technology was used to compare the sequences between a wheat-Thinopyrum 1St#2 (1D) substitution and the related species Pseudoroegneria spicata (St genome, 2n = 14). A total of 5142 polymorphic fragments were analyzed and 359 different SLAF markers for 1St#2 were predicted. Thirty-seven specific molecular markers were validated by PCR from 50 randomly selected SLAFs. Meanwhile, the distribution of transposable elements (TEs) at the family level between wheat and St genomes was compared using the SLAFs. A new oligo-nucleotide probe named Oligo-pSt122 from high SLAF reads was produced for fluorescence in situ hybridization (FISH), and was observed to hybridize to the terminal region of 1St#L and also onto the terminal heterochromatic region of Th. intermedium genomes. The genome-wide markers and repetitive based probe Oligo-pSt122 will be valuable for identifying Thinopyrum chromosome segments in wheat backgrounds.

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Publisher Full Text (DOI)

Authors+Show Affiliations

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China.

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China.

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China.

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China.

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China.

Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan, China. yangzujun@uestc.edu.cn.

MeSH

Chromosomes, PlantDNA ProbesDNA Transposable ElementsGenetic MarkersGenome, PlantGlutensHigh-Throughput Nucleotide SequencingHybridization, GeneticIn Situ Hybridization, FluorescencePlant BreedingPlant ProteinsPoaceaeSpecies SpecificityTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27290728

Citation

Li, Guangrong, et al. "New Molecular Markers and Cytogenetic Probes Enable Chromosome Identification of wheat-Thinopyrum Intermedium Introgression Lines for Improving Protein and Gluten Contents." Planta, vol. 244, no. 4, 2016, pp. 865-76.

Li G, Wang H, Lang T, et al. New molecular markers and cytogenetic probes enable chromosome identification of wheat-Thinopyrum intermedium introgression lines for improving protein and gluten contents. Planta. 2016;244(4):865-76.

Li, G., Wang, H., Lang, T., Li, J., La, S., Yang, E., & Yang, Z. (2016). New molecular markers and cytogenetic probes enable chromosome identification of wheat-Thinopyrum intermedium introgression lines for improving protein and gluten contents. Planta, 244(4), 865-76. https://doi.org/10.1007/s00425-016-2554-y

Li G, et al. New Molecular Markers and Cytogenetic Probes Enable Chromosome Identification of wheat-Thinopyrum Intermedium Introgression Lines for Improving Protein and Gluten Contents. Planta. 2016;244(4):865-76. PubMed PMID: 27290728.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - New molecular markers and cytogenetic probes enable chromosome identification of wheat-Thinopyrum intermedium introgression lines for improving protein and gluten contents. AU - Li,Guangrong, AU - Wang,Hongjin, AU - Lang,Tao, AU - Li,Jianbo, AU - La,Shixiao, AU - Yang,Ennian, AU - Yang,Zujun, Y1 - 2016/06/11/ PY - 2016/03/03/received PY - 2016/06/06/accepted PY - 2016/6/13/entrez PY - 2016/6/13/pubmed PY - 2017/4/11/medline KW - Grain quality KW - In situ hybridization KW - Molecular markers KW - Thinopyrum intermedium KW - Wheat SP - 865 EP - 76 JF - Planta JO - Planta VL - 244 IS - 4 N2 - MAIN CONCLUSION: New molecular markers were developed for targeting Thinopyrum intermedium 1St#2 chromosome, and novel FISH probe representing the terminal repeats was produced for identification of Thinopyrum chromosomes. Thinopyrum intermedium has been used as a valuable resource for improving the disease resistance and yield potential of wheat. A wheat-Th. intermedium ssp. trichophorum chromosome 1St#2 substitution and translocation has displayed superior grain protein and wet gluten content. With the aim to develop a number of chromosome 1St#2 specific molecular and cytogenetic markers, a high throughput, low-cost specific-locus amplified fragment sequencing (SLAF-seq) technology was used to compare the sequences between a wheat-Thinopyrum 1St#2 (1D) substitution and the related species Pseudoroegneria spicata (St genome, 2n = 14). A total of 5142 polymorphic fragments were analyzed and 359 different SLAF markers for 1St#2 were predicted. Thirty-seven specific molecular markers were validated by PCR from 50 randomly selected SLAFs. Meanwhile, the distribution of transposable elements (TEs) at the family level between wheat and St genomes was compared using the SLAFs. A new oligo-nucleotide probe named Oligo-pSt122 from high SLAF reads was produced for fluorescence in situ hybridization (FISH), and was observed to hybridize to the terminal region of 1St#L and also onto the terminal heterochromatic region of Th. intermedium genomes. The genome-wide markers and repetitive based probe Oligo-pSt122 will be valuable for identifying Thinopyrum chromosome segments in wheat backgrounds. SN - 1432-2048 UR - https://www.unboundmedicine.com/medline/citation/27290728/New_molecular_markers_and_cytogenetic_probes_enable_chromosome_identification_of_wheat_Thinopyrum_intermedium_introgression_lines_for_improving_protein_and_gluten_contents_ DB - PRIME DP - Unbound Medicine ER -