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Protective Effect of Ginsenoside Rg1 on Hematopoietic Stem/Progenitor Cells through Attenuating Oxidative Stress and the Wnt/β-Catenin Signaling Pathway in a Mouse Model of d-Galactose-induced Aging.
Int J Mol Sci. 2016 Jun 09; 17(6)IJ

Abstract

Stem cell senescence is an important and current hypothesis accounting for organismal aging, especially the hematopoietic stem cell (HSC). Ginsenoside Rg1 is the main active pharmaceutical ingredient of ginseng, which is a traditional Chinese medicine. This study explored the protective effect of ginsenoside Rg1 on Sca-1⁺ hematopoietic stem/progenitor cells (HSC/HPCs) in a mouse model of d-galactose-induced aging. The mimetic aging mouse model was induced by continuous injection of d-gal for 42 days, and the C57BL/6 mice were respectively treated with ginsenoside Rg1, Vitamin E or normal saline after 7 days of d-gal injection. Compared with those in the d-gal administration alone group, ginsenoside Rg1 protected Sca-1⁺ HSC/HPCs by decreasing SA-β-Gal and enhancing the colony forming unit-mixture (CFU-Mix), and adjusting oxidative stress indices like reactive oxygen species (ROS), total anti-oxidant (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and malondialdehyde (MDA). In addition, ginsenoside Rg1 decreased β-catenin and c-Myc mRNA expression and enhanced the phosphorylation of GSK-3β. Moreover, ginsenoside Rg1 down-regulated advanced glycation end products (AGEs), 4-hydroxynonenal (4-HNE), phospho-histone H2A.X (r-H2A.X), 8-OHdG, p16(Ink4a), Rb, p21(Cip1/Waf1) and p53 in senescent Sca-1⁺ HSC/HPCs. Our findings indicated that ginsenoside Rg1 can improve the resistance of Sca-1⁺ HSC/HPCs in a mouse model of d-galactose-induced aging through the suppression of oxidative stress and excessive activation of the Wnt/β-catenin signaling pathway, and reduction of DNA damage response, p16(Ink4a)-Rb and p53-p21(Cip1/Waf1) signaling.

Authors+Show Affiliations

Laboratory of Stem Cells and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China. jingli2523@gmail.com. Department of Pathophysiology, Chongqing Medical University, Chongqing 400016, China. jingli2523@gmail.com.Department of Infectious Diseases, the Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China. cqmucdc@sohu.com.Department of Pathophysiology, Chongqing Medical University, Chongqing 400016, China. moliliuxiang@aliyun.com.Laboratory of Stem Cells and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China. cqzyi531@gmail.com.Laboratory of Stem Cells and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China. cqcb9866@outlook.com.Laboratory of Stem Cells and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China. xhjke0316@gmail.com.Laboratory of Stem Cells and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China. wanglu99@sina.com. Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China. wanglu99@sina.com.Laboratory of Stem Cells and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China. ypwangcq@aliyun.com. Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China. ypwangcq@aliyun.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27294914

Citation

Li, Jing, et al. "Protective Effect of Ginsenoside Rg1 On Hematopoietic Stem/Progenitor Cells Through Attenuating Oxidative Stress and the Wnt/β-Catenin Signaling Pathway in a Mouse Model of d-Galactose-induced Aging." International Journal of Molecular Sciences, vol. 17, no. 6, 2016.
Li J, Cai D, Yao X, et al. Protective Effect of Ginsenoside Rg1 on Hematopoietic Stem/Progenitor Cells through Attenuating Oxidative Stress and the Wnt/β-Catenin Signaling Pathway in a Mouse Model of d-Galactose-induced Aging. Int J Mol Sci. 2016;17(6).
Li, J., Cai, D., Yao, X., Zhang, Y., Chen, L., Jing, P., Wang, L., & Wang, Y. (2016). Protective Effect of Ginsenoside Rg1 on Hematopoietic Stem/Progenitor Cells through Attenuating Oxidative Stress and the Wnt/β-Catenin Signaling Pathway in a Mouse Model of d-Galactose-induced Aging. International Journal of Molecular Sciences, 17(6). https://doi.org/10.3390/ijms17060849
Li J, et al. Protective Effect of Ginsenoside Rg1 On Hematopoietic Stem/Progenitor Cells Through Attenuating Oxidative Stress and the Wnt/β-Catenin Signaling Pathway in a Mouse Model of d-Galactose-induced Aging. Int J Mol Sci. 2016 Jun 9;17(6) PubMed PMID: 27294914.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective Effect of Ginsenoside Rg1 on Hematopoietic Stem/Progenitor Cells through Attenuating Oxidative Stress and the Wnt/β-Catenin Signaling Pathway in a Mouse Model of d-Galactose-induced Aging. AU - Li,Jing, AU - Cai,Dachuan, AU - Yao,Xin, AU - Zhang,Yanyan, AU - Chen,Linbo, AU - Jing,Pengwei, AU - Wang,Lu, AU - Wang,Yaping, Y1 - 2016/06/09/ PY - 2016/04/18/received PY - 2016/05/19/revised PY - 2016/05/25/accepted PY - 2016/6/14/entrez PY - 2016/6/15/pubmed PY - 2017/3/16/medline KW - ">d-galactose KW - Wnt/β-catenin KW - cellular senescence KW - ginsenoside Rg1 KW - hematopoietic stem/progenitor cell (HSC/HPC) KW - oxidative stress JF - International journal of molecular sciences JO - Int J Mol Sci VL - 17 IS - 6 N2 - Stem cell senescence is an important and current hypothesis accounting for organismal aging, especially the hematopoietic stem cell (HSC). Ginsenoside Rg1 is the main active pharmaceutical ingredient of ginseng, which is a traditional Chinese medicine. This study explored the protective effect of ginsenoside Rg1 on Sca-1⁺ hematopoietic stem/progenitor cells (HSC/HPCs) in a mouse model of d-galactose-induced aging. The mimetic aging mouse model was induced by continuous injection of d-gal for 42 days, and the C57BL/6 mice were respectively treated with ginsenoside Rg1, Vitamin E or normal saline after 7 days of d-gal injection. Compared with those in the d-gal administration alone group, ginsenoside Rg1 protected Sca-1⁺ HSC/HPCs by decreasing SA-β-Gal and enhancing the colony forming unit-mixture (CFU-Mix), and adjusting oxidative stress indices like reactive oxygen species (ROS), total anti-oxidant (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and malondialdehyde (MDA). In addition, ginsenoside Rg1 decreased β-catenin and c-Myc mRNA expression and enhanced the phosphorylation of GSK-3β. Moreover, ginsenoside Rg1 down-regulated advanced glycation end products (AGEs), 4-hydroxynonenal (4-HNE), phospho-histone H2A.X (r-H2A.X), 8-OHdG, p16(Ink4a), Rb, p21(Cip1/Waf1) and p53 in senescent Sca-1⁺ HSC/HPCs. Our findings indicated that ginsenoside Rg1 can improve the resistance of Sca-1⁺ HSC/HPCs in a mouse model of d-galactose-induced aging through the suppression of oxidative stress and excessive activation of the Wnt/β-catenin signaling pathway, and reduction of DNA damage response, p16(Ink4a)-Rb and p53-p21(Cip1/Waf1) signaling. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/27294914/Protective_Effect_of_Ginsenoside_Rg1_on_Hematopoietic_Stem/Progenitor_Cells_through_Attenuating_Oxidative_Stress_and_the_Wnt/β_Catenin_Signaling_Pathway_in_a_Mouse_Model_of_d_Galactose_induced_Aging_ L2 - http://www.mdpi.com/resolver?pii=ijms17060849 DB - PRIME DP - Unbound Medicine ER -