Tags

Type your tag names separated by a space and hit enter

Faecalibacterium prausnitzii supernatant ameliorates dextran sulfate sodium induced colitis by regulating Th17 cell differentiation.
World J Gastroenterol. 2016 Jun 14; 22(22):5201-10.WJ

Abstract

AIM

To explore the preventive and therapeutic effects of Faecalibacterium prausnitzii (F. prausnitzii) supernatant on dextran sulfate sodium (DSS) induced colitis in mice.

METHODS

Forty C57BL/6J male mice were randomly divided into four groups: control group, model group, treatment group, and prevention group. Mice were weighed daily. On day 10, the colon length was measured, the colorectal histopathologic damage score (HDS) was assessed, and plasma interleukin (IL)-17A, IL-6, and IL-4 levels were detected by enzyme-linked immunosorbent assay. The expression of transcription factor retinoic acid-related orphan receptor-γt (RORγt) and IL-17A in colon inflammatory mucosa tissue were determined by immunohistochemical assay, and the expression levels of RORγt mRNA, IL-17A mRNA, and IL-6 mRNA were detected by real-time quantitative polymerase chain reaction (PCR). The proportion of Th17 in mononuclear cells in spleen was assayed by fluorescence activated cell sorter.

RESULTS

When compared with the model group, the colon length (P < 0.05) and body weight (P < 0.01) in the treatment and prevention groups were significantly increased, and the colon HDS was decreased (P < 0.05 and P < 0.01). There was no statistical difference between the treatment group and prevention group. After treatment with F. prausnitzii supernatant, the plasma levels of IL-17A and IL-6 (P < 0.05), the protein and mRNA expression of IL-17A and RORγt, and the Th17 cell ratio of spleen cells (P < 0.01) were significantly decreased compared to the model group. Plasma IL-4 level in the prevention group was significantly higher than that in the model group (P < 0.05), but there was no significant difference between these two groups in the expression of IL-6 in both the plasma and colon mucosa tissues.

CONCLUSION

F. prausnitzii supernatant exerts protective and therapeutic effects on DSS-induced colitis in mice, probably via inhibition of Th17 differentiation and IL-17A secretion in the plasma and colon mucosa tissues. It can also improve colitis in mice by downregulating IL-6 and prevent colitis by upregulating IL-4.

Authors+Show Affiliations

Xiao-Li Huang, Yan-Ping Hao, Yan-Qiu Yu, Cheng-Gong Yu, Department of Gastroenterology, Gulou School of Clinical Medicine, Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.Xiao-Li Huang, Yan-Ping Hao, Yan-Qiu Yu, Cheng-Gong Yu, Department of Gastroenterology, Gulou School of Clinical Medicine, Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.Xiao-Li Huang, Yan-Ping Hao, Yan-Qiu Yu, Cheng-Gong Yu, Department of Gastroenterology, Gulou School of Clinical Medicine, Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.Xiao-Li Huang, Yan-Ping Hao, Yan-Qiu Yu, Cheng-Gong Yu, Department of Gastroenterology, Gulou School of Clinical Medicine, Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.Xiao-Li Huang, Yan-Ping Hao, Yan-Qiu Yu, Cheng-Gong Yu, Department of Gastroenterology, Gulou School of Clinical Medicine, Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.Xiao-Li Huang, Yan-Ping Hao, Yan-Qiu Yu, Cheng-Gong Yu, Department of Gastroenterology, Gulou School of Clinical Medicine, Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.Xiao-Li Huang, Yan-Ping Hao, Yan-Qiu Yu, Cheng-Gong Yu, Department of Gastroenterology, Gulou School of Clinical Medicine, Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.Xiao-Li Huang, Yan-Ping Hao, Yan-Qiu Yu, Cheng-Gong Yu, Department of Gastroenterology, Gulou School of Clinical Medicine, Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.Xiao-Li Huang, Yan-Ping Hao, Yan-Qiu Yu, Cheng-Gong Yu, Department of Gastroenterology, Gulou School of Clinical Medicine, Nanjing Medical University, Nanjing 210008, Jiangsu Province, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27298563

Citation

Huang, Xiao-Li, et al. "Faecalibacterium Prausnitzii Supernatant Ameliorates Dextran Sulfate Sodium Induced Colitis By Regulating Th17 Cell Differentiation." World Journal of Gastroenterology, vol. 22, no. 22, 2016, pp. 5201-10.
Huang XL, Zhang X, Fei XY, et al. Faecalibacterium prausnitzii supernatant ameliorates dextran sulfate sodium induced colitis by regulating Th17 cell differentiation. World J Gastroenterol. 2016;22(22):5201-10.
Huang, X. L., Zhang, X., Fei, X. Y., Chen, Z. G., Hao, Y. P., Zhang, S., Zhang, M. M., Yu, Y. Q., & Yu, C. G. (2016). Faecalibacterium prausnitzii supernatant ameliorates dextran sulfate sodium induced colitis by regulating Th17 cell differentiation. World Journal of Gastroenterology, 22(22), 5201-10. https://doi.org/10.3748/wjg.v22.i22.5201
Huang XL, et al. Faecalibacterium Prausnitzii Supernatant Ameliorates Dextran Sulfate Sodium Induced Colitis By Regulating Th17 Cell Differentiation. World J Gastroenterol. 2016 Jun 14;22(22):5201-10. PubMed PMID: 27298563.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Faecalibacterium prausnitzii supernatant ameliorates dextran sulfate sodium induced colitis by regulating Th17 cell differentiation. AU - Huang,Xiao-Li, AU - Zhang,Xin, AU - Fei,Xian-Yan, AU - Chen,Zhao-Gui, AU - Hao,Yan-Ping, AU - Zhang,Shu, AU - Zhang,Ming-Ming, AU - Yu,Yan-Qiu, AU - Yu,Cheng-Gong, PY - 2016/01/23/received PY - 2016/02/28/revised PY - 2016/03/18/accepted PY - 2016/6/15/entrez PY - 2016/6/15/pubmed PY - 2017/4/11/medline KW - Animal model KW - Faecalibacterium prausnitzii KW - Prevention KW - Th17 cell KW - Treatment KW - Ulcerative colitis SP - 5201 EP - 10 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 22 IS - 22 N2 - AIM: To explore the preventive and therapeutic effects of Faecalibacterium prausnitzii (F. prausnitzii) supernatant on dextran sulfate sodium (DSS) induced colitis in mice. METHODS: Forty C57BL/6J male mice were randomly divided into four groups: control group, model group, treatment group, and prevention group. Mice were weighed daily. On day 10, the colon length was measured, the colorectal histopathologic damage score (HDS) was assessed, and plasma interleukin (IL)-17A, IL-6, and IL-4 levels were detected by enzyme-linked immunosorbent assay. The expression of transcription factor retinoic acid-related orphan receptor-γt (RORγt) and IL-17A in colon inflammatory mucosa tissue were determined by immunohistochemical assay, and the expression levels of RORγt mRNA, IL-17A mRNA, and IL-6 mRNA were detected by real-time quantitative polymerase chain reaction (PCR). The proportion of Th17 in mononuclear cells in spleen was assayed by fluorescence activated cell sorter. RESULTS: When compared with the model group, the colon length (P < 0.05) and body weight (P < 0.01) in the treatment and prevention groups were significantly increased, and the colon HDS was decreased (P < 0.05 and P < 0.01). There was no statistical difference between the treatment group and prevention group. After treatment with F. prausnitzii supernatant, the plasma levels of IL-17A and IL-6 (P < 0.05), the protein and mRNA expression of IL-17A and RORγt, and the Th17 cell ratio of spleen cells (P < 0.01) were significantly decreased compared to the model group. Plasma IL-4 level in the prevention group was significantly higher than that in the model group (P < 0.05), but there was no significant difference between these two groups in the expression of IL-6 in both the plasma and colon mucosa tissues. CONCLUSION: F. prausnitzii supernatant exerts protective and therapeutic effects on DSS-induced colitis in mice, probably via inhibition of Th17 differentiation and IL-17A secretion in the plasma and colon mucosa tissues. It can also improve colitis in mice by downregulating IL-6 and prevent colitis by upregulating IL-4. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/27298563/Faecalibacterium_prausnitzii_supernatant_ameliorates_dextran_sulfate_sodium_induced_colitis_by_regulating_Th17_cell_differentiation_ L2 - http://www.wjgnet.com/1007-9327/full/v22/i22/5201.htm DB - PRIME DP - Unbound Medicine ER -