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OsWRKY51, a rice transcription factor, functions as a positive regulator in defense response against Xanthomonas oryzae pv. oryzae.
Plant Cell Rep 2016; 35(9):1975-85PC

Abstract

KEY MESSAGE

OsWRKY51 functions as a positive transcriptional regulator in defense signaling against Xanthomonas oryzae pv. oryzae by direct DNA binding to the promoter of defense related gene, OsPR10a. OsWRKY51 in rice (Oryza sativa L.) is induced by exogenous salicylic acid (SA) and inoculation with Xanthomonas oryzae pv. oryzae (Xoo). To examine the role of OsWRKY51 in the defense response of rice, we generated OsWRKY51 overexpressing and underexpressing transgenic rice plants. OsWRKY51-overexpressing transgenic rice lines were more resistant to Xoo and showed greater expression of defense-related genes than wild-type (WT) plants, while OsWRKY51-underexpressing lines were more susceptible to Xoo and showed less expression of defense-associated genes than WT plants. Transgenic lines overexpressing OsWRKY51 showed growth retardation compared to WT plants. In contrast, transgenic lines underexpressing OsWRKY51 by RNA interference showed similar plant height with WT plants. Transient expression of OsWRKY51-green fluorescent protein fusion protein in rice protoplasts revealed that OsWRKY51 was localized in the nucleus. OsWRKY51 bound to the W-box and WLE1 elements of the OsPR10a promoter. Based on these results, we suggest that OsWRKY51 is a positive transcriptional regulator of defense signaling and has direct DNA binding ability to the promoter of OsPR10a, although it is reported to be a negative regulator in GA signaling.

Authors+Show Affiliations

National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea. Convergence Research Center for Functional Plant Products, Advanced Institutes of Convergence Technology (AICT), Suwon, 443-270, Korea.National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea. djhwang@rda.go.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27300023

Citation

Hwang, Seon-Hee, et al. "OsWRKY51, a Rice Transcription Factor, Functions as a Positive Regulator in Defense Response Against Xanthomonas Oryzae Pv. Oryzae." Plant Cell Reports, vol. 35, no. 9, 2016, pp. 1975-85.
Hwang SH, Kwon SI, Jang JY, et al. OsWRKY51, a rice transcription factor, functions as a positive regulator in defense response against Xanthomonas oryzae pv. oryzae. Plant Cell Rep. 2016;35(9):1975-85.
Hwang, S. H., Kwon, S. I., Jang, J. Y., Fang, I. L., Lee, H., Choi, C., ... Hwang, D. J. (2016). OsWRKY51, a rice transcription factor, functions as a positive regulator in defense response against Xanthomonas oryzae pv. oryzae. Plant Cell Reports, 35(9), pp. 1975-85. doi:10.1007/s00299-016-2012-0.
Hwang SH, et al. OsWRKY51, a Rice Transcription Factor, Functions as a Positive Regulator in Defense Response Against Xanthomonas Oryzae Pv. Oryzae. Plant Cell Rep. 2016;35(9):1975-85. PubMed PMID: 27300023.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - OsWRKY51, a rice transcription factor, functions as a positive regulator in defense response against Xanthomonas oryzae pv. oryzae. AU - Hwang,Seon-Hee, AU - Kwon,Soon Il, AU - Jang,Ji-Young, AU - Fang,Il Lan, AU - Lee,Heyoung, AU - Choi,Changhyun, AU - Park,Sangryeol, AU - Ahn,Ilpyung, AU - Bae,Shin-Chul, AU - Hwang,Duk-Ju, Y1 - 2016/06/14/ PY - 2016/04/09/received PY - 2016/06/04/accepted PY - 2016/6/15/entrez PY - 2016/6/15/pubmed PY - 2017/3/28/medline KW - DNA binding KW - Defense KW - OsWRKY51 KW - Xanthomonas oryzae pv. oryzae SP - 1975 EP - 85 JF - Plant cell reports JO - Plant Cell Rep. VL - 35 IS - 9 N2 - KEY MESSAGE: OsWRKY51 functions as a positive transcriptional regulator in defense signaling against Xanthomonas oryzae pv. oryzae by direct DNA binding to the promoter of defense related gene, OsPR10a. OsWRKY51 in rice (Oryza sativa L.) is induced by exogenous salicylic acid (SA) and inoculation with Xanthomonas oryzae pv. oryzae (Xoo). To examine the role of OsWRKY51 in the defense response of rice, we generated OsWRKY51 overexpressing and underexpressing transgenic rice plants. OsWRKY51-overexpressing transgenic rice lines were more resistant to Xoo and showed greater expression of defense-related genes than wild-type (WT) plants, while OsWRKY51-underexpressing lines were more susceptible to Xoo and showed less expression of defense-associated genes than WT plants. Transgenic lines overexpressing OsWRKY51 showed growth retardation compared to WT plants. In contrast, transgenic lines underexpressing OsWRKY51 by RNA interference showed similar plant height with WT plants. Transient expression of OsWRKY51-green fluorescent protein fusion protein in rice protoplasts revealed that OsWRKY51 was localized in the nucleus. OsWRKY51 bound to the W-box and WLE1 elements of the OsPR10a promoter. Based on these results, we suggest that OsWRKY51 is a positive transcriptional regulator of defense signaling and has direct DNA binding ability to the promoter of OsPR10a, although it is reported to be a negative regulator in GA signaling. SN - 1432-203X UR - https://www.unboundmedicine.com/medline/citation/27300023/OsWRKY51_a_rice_transcription_factor_functions_as_a_positive_regulator_in_defense_response_against_Xanthomonas_oryzae_pv__oryzae_ L2 - http://dx.doi.org/10.1007/s00299-016-2012-0 DB - PRIME DP - Unbound Medicine ER -