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Diversification of the Homoeologous Lr34 Sequences in Polyploid Wheat Species and Their Diploid Progenitors.
J Mol Evol. 2016 06; 82(6):291-302.JM

Abstract

Allopolyploidization induces a multiple processes of genomic reorganization, including the structurally functional diversification of the homoeologous genes. An example of such diversification is the appearance of the Lr34 gene on chromosome 7D of bread wheat T. aestivum (BAD), the gene conferring durable, race non-specific protection against three fungal pathogens. In this study, we focused on the variability of a functionally critical region between exons 10-12 of Lr34 among diploid progenitors of wheat genomes and their respective polyploids. In the diploid A-genome species, two basic forms of the studied region have been revealed: (1) non-functional forms containing stop codons, or/and frameshifts (T. monococcum/T. urartu) and (2) forms with no such a mutations (T. boeoticum). The Lr34 sequence of T. urartu containing a TGA stop codon was inherited by the first tetraploid T. dicoccoides (BA), and then reorganized in some accessions of this species due to the insertion of an LTR retroelement in exon 10. Besides T. boeoticum, the second form of the Lr34 sequence is also characteristic of A. speltoides, which presumably donated this form to all polyploid descendants bearing B-genome. No differences were found between the D-genome-specific Lr34 sequences studied here and downloaded from databases, implying the highest level of conservation of the Lr34 predecessor throughout evolution. The sequence data were later used to construct phylograms, and apparent peculiarities in the evolution of the studied region of Lr34 genes discussed.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Shcherban AB
The Federal Research Center "Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences", Lavrentiev ave. 10,, Novosibirsk, Russia, 630090. atos@bionet.nsc.ru.
Kochieva EZ
Federal State Institution «Federal Research Centre «Fundamentals of Biotechnology» of the Russian Academy of Sciences, Moscow, Russia.
Salina EA
The Federal Research Center "Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences", Lavrentiev ave. 10,, Novosibirsk, Russia, 630090.

MeSH

AllelesChromosome MappingChromosomes, PlantDiploidyGenes, PlantGenome, PlantPhylogenyPolyploidyRetroelementsSequence HomologyTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27300207

Citation

Shcherban, A B., et al. "Diversification of the Homoeologous Lr34 Sequences in Polyploid Wheat Species and Their Diploid Progenitors." Journal of Molecular Evolution, vol. 82, no. 6, 2016, pp. 291-302.
Shcherban AB, Kochieva EZ, Salina EA. Diversification of the Homoeologous Lr34 Sequences in Polyploid Wheat Species and Their Diploid Progenitors. J Mol Evol. 2016;82(6):291-302.
Shcherban, A. B., Kochieva, E. Z., & Salina, E. A. (2016). Diversification of the Homoeologous Lr34 Sequences in Polyploid Wheat Species and Their Diploid Progenitors. Journal of Molecular Evolution, 82(6), 291-302. https://doi.org/10.1007/s00239-016-9748-6
Shcherban AB, Kochieva EZ, Salina EA. Diversification of the Homoeologous Lr34 Sequences in Polyploid Wheat Species and Their Diploid Progenitors. J Mol Evol. 2016;82(6):291-302. PubMed PMID: 27300207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversification of the Homoeologous Lr34 Sequences in Polyploid Wheat Species and Their Diploid Progenitors. AU - Shcherban,A B, AU - Kochieva,E Z, AU - Salina,E A, Y1 - 2016/06/14/ PY - 2016/03/15/received PY - 2016/06/04/accepted PY - 2016/6/15/entrez PY - 2016/6/15/pubmed PY - 2017/6/2/medline KW - ABCG transporter KW - DNA sequence KW - Gene evolution KW - Genome KW - Polyploid KW - Retroelement KW - Wheat SP - 291 EP - 302 JF - Journal of molecular evolution JO - J Mol Evol VL - 82 IS - 6 N2 - Allopolyploidization induces a multiple processes of genomic reorganization, including the structurally functional diversification of the homoeologous genes. An example of such diversification is the appearance of the Lr34 gene on chromosome 7D of bread wheat T. aestivum (BAD), the gene conferring durable, race non-specific protection against three fungal pathogens. In this study, we focused on the variability of a functionally critical region between exons 10-12 of Lr34 among diploid progenitors of wheat genomes and their respective polyploids. In the diploid A-genome species, two basic forms of the studied region have been revealed: (1) non-functional forms containing stop codons, or/and frameshifts (T. monococcum/T. urartu) and (2) forms with no such a mutations (T. boeoticum). The Lr34 sequence of T. urartu containing a TGA stop codon was inherited by the first tetraploid T. dicoccoides (BA), and then reorganized in some accessions of this species due to the insertion of an LTR retroelement in exon 10. Besides T. boeoticum, the second form of the Lr34 sequence is also characteristic of A. speltoides, which presumably donated this form to all polyploid descendants bearing B-genome. No differences were found between the D-genome-specific Lr34 sequences studied here and downloaded from databases, implying the highest level of conservation of the Lr34 predecessor throughout evolution. The sequence data were later used to construct phylograms, and apparent peculiarities in the evolution of the studied region of Lr34 genes discussed. SN - 1432-1432 UR - https://www.unboundmedicine.com/medline/citation/27300207/Diversification_of_the_Homoeologous_Lr34_Sequences_in_Polyploid_Wheat_Species_and_Their_Diploid_Progenitors_ DB - PRIME DP - Unbound Medicine ER -