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Pellet feed adsorbed with the recombinant Lactococcus lactis BFE920 expressing SiMA antigen induced strong recall vaccine effects against Streptococcus iniae infection in olive flounder (Paralichthys olivaceus).
Fish Shellfish Immunol. 2016 Aug; 55:374-83.FS

Abstract

The aim of this study was to develop a fish feed vaccine that provides effective disease prevention and convenient application. A lactic acid bacterium (LAB), Lactococcus lactis BFE920, was modified to express the SiMA antigen, a membrane protein of Streptococcus iniae. The antigen was engineered to be expressed under the nisin promoter, which is induced by nisin produced naturally by the host LAB. Various sizes (40 ± 3.5 g, 80 ± 2.1 g, and 221 ± 2.4 g) of olive flounder (Paralichthys olivaceus) were vaccinated by feeding the extruded pellet feed, onto which the SiMA-expressing L. lactis BFE920 (1.0 × 10(7) CFU/g) was adsorbed. Vaccine-treated feed was administered twice a day for 1 week, and priming and boosting were performed with a 1-week interval in between. The vaccinated fish had significantly elevated levels of antigen-specific serum antibodies and T cell marker mRNAs: CD4-1, CD4-2, and CD8a. In addition, the feed vaccine significantly induced T cell effector functions, such as the production of IFN-γ and activation of the transcription factor that induces its expression, T-bet. When the flounder were challenged by intraperitoneal infection and bath immersion with S. iniae, the vaccinated fish showed 84% and 82% relative percent survival (RPS), respectively. Furthermore, similar protective effects were confirmed even 3 months after vaccination in a field study (n = 4800), indicating that this feed vaccine elicited prolonged duration of immunopotency. In addition, the vaccinated flounder gained 21% more weight and required 16% less feed to gain a unit of body weight compared to the control group. The data clearly demonstrate that the L. lactis BFE920-SiMA feed vaccine has strong protective effects, induces prolonged vaccine efficacy, and has probiotic effects. In addition, this LAB-based fish feed vaccine can be easily used to target many different pathogens of diverse fish species.

Authors+Show Affiliations

School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Gyeonggi-do, Republic of Korea.New Business Development Dept., Medytox Inc, Republic of Korea.Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.GyeongSangbuk-Do Fisheries Technology Center, Pohang 791-941, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea. Electronic address: sksong@handong.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27302864

Citation

Kim, Daniel, et al. "Pellet Feed Adsorbed With the Recombinant Lactococcus Lactis BFE920 Expressing SiMA Antigen Induced Strong Recall Vaccine Effects Against Streptococcus Iniae Infection in Olive Flounder (Paralichthys Olivaceus)." Fish & Shellfish Immunology, vol. 55, 2016, pp. 374-83.
Kim D, Beck BR, Lee SM, et al. Pellet feed adsorbed with the recombinant Lactococcus lactis BFE920 expressing SiMA antigen induced strong recall vaccine effects against Streptococcus iniae infection in olive flounder (Paralichthys olivaceus). Fish Shellfish Immunol. 2016;55:374-83.
Kim, D., Beck, B. R., Lee, S. M., Jeon, J., Lee, D. W., Lee, J. I., & Song, S. K. (2016). Pellet feed adsorbed with the recombinant Lactococcus lactis BFE920 expressing SiMA antigen induced strong recall vaccine effects against Streptococcus iniae infection in olive flounder (Paralichthys olivaceus). Fish & Shellfish Immunology, 55, 374-83. https://doi.org/10.1016/j.fsi.2016.06.010
Kim D, et al. Pellet Feed Adsorbed With the Recombinant Lactococcus Lactis BFE920 Expressing SiMA Antigen Induced Strong Recall Vaccine Effects Against Streptococcus Iniae Infection in Olive Flounder (Paralichthys Olivaceus). Fish Shellfish Immunol. 2016;55:374-83. PubMed PMID: 27302864.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pellet feed adsorbed with the recombinant Lactococcus lactis BFE920 expressing SiMA antigen induced strong recall vaccine effects against Streptococcus iniae infection in olive flounder (Paralichthys olivaceus). AU - Kim,Daniel, AU - Beck,Bo Ram, AU - Lee,Sun Min, AU - Jeon,Jongsu, AU - Lee,Dong Wook, AU - Lee,Jae Il, AU - Song,Seong Kyu, Y1 - 2016/06/11/ PY - 2016/04/25/received PY - 2016/05/31/revised PY - 2016/06/06/accepted PY - 2016/6/16/entrez PY - 2016/6/16/pubmed PY - 2017/2/18/medline KW - Feed vaccine KW - Lactococcus lactis BFE920 KW - Olive flounder KW - Oral immunization KW - Streptococcosis KW - Streptococcus iniae KW - Vaccine vehicle SP - 374 EP - 83 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 55 N2 - The aim of this study was to develop a fish feed vaccine that provides effective disease prevention and convenient application. A lactic acid bacterium (LAB), Lactococcus lactis BFE920, was modified to express the SiMA antigen, a membrane protein of Streptococcus iniae. The antigen was engineered to be expressed under the nisin promoter, which is induced by nisin produced naturally by the host LAB. Various sizes (40 ± 3.5 g, 80 ± 2.1 g, and 221 ± 2.4 g) of olive flounder (Paralichthys olivaceus) were vaccinated by feeding the extruded pellet feed, onto which the SiMA-expressing L. lactis BFE920 (1.0 × 10(7) CFU/g) was adsorbed. Vaccine-treated feed was administered twice a day for 1 week, and priming and boosting were performed with a 1-week interval in between. The vaccinated fish had significantly elevated levels of antigen-specific serum antibodies and T cell marker mRNAs: CD4-1, CD4-2, and CD8a. In addition, the feed vaccine significantly induced T cell effector functions, such as the production of IFN-γ and activation of the transcription factor that induces its expression, T-bet. When the flounder were challenged by intraperitoneal infection and bath immersion with S. iniae, the vaccinated fish showed 84% and 82% relative percent survival (RPS), respectively. Furthermore, similar protective effects were confirmed even 3 months after vaccination in a field study (n = 4800), indicating that this feed vaccine elicited prolonged duration of immunopotency. In addition, the vaccinated flounder gained 21% more weight and required 16% less feed to gain a unit of body weight compared to the control group. The data clearly demonstrate that the L. lactis BFE920-SiMA feed vaccine has strong protective effects, induces prolonged vaccine efficacy, and has probiotic effects. In addition, this LAB-based fish feed vaccine can be easily used to target many different pathogens of diverse fish species. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/27302864/Pellet_feed_adsorbed_with_the_recombinant_Lactococcus_lactis_BFE920_expressing_SiMA_antigen_induced_strong_recall_vaccine_effects_against_Streptococcus_iniae_infection_in_olive_flounder__Paralichthys_olivaceus__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(16)30370-9 DB - PRIME DP - Unbound Medicine ER -