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No Identical "Mesenchymal Stem Cells" at Different Times and Sites: Human Committed Progenitors of Distinct Origin and Differentiation Potential Are Incorporated as Adventitial Cells in Microvessels.
Stem Cell Reports 2016; 6(6):897-913SC

Abstract

A widely shared view reads that mesenchymal stem/stromal cells ("MSCs") are ubiquitous in human connective tissues, can be defined by a common in vitro phenotype, share a skeletogenic potential as assessed by in vitro differentiation assays, and coincide with ubiquitous pericytes. Using stringent in vivo differentiation assays and transcriptome analysis, we show that human cell populations from different anatomical sources, regarded as "MSCs" based on these criteria and assumptions, actually differ widely in their transcriptomic signature and in vivo differentiation potential. In contrast, they share the capacity to guide the assembly of functional microvessels in vivo, regardless of their anatomical source, or in situ identity as perivascular or circulating cells. This analysis reveals that muscle pericytes, which are not spontaneously osteochondrogenic as previously claimed, may indeed coincide with an ectopic perivascular subset of committed myogenic cells similar to satellite cells. Cord blood-derived stromal cells, on the other hand, display the unique capacity to form cartilage in vivo spontaneously, in addition to an assayable osteogenic capacity. These data suggest the need to revise current misconceptions on the origin and function of so-called "MSCs," with important applicative implications. The data also support the view that rather than a uniform class of "MSCs," different mesoderm derivatives include distinct classes of tissue-specific committed progenitors, possibly of different developmental origin.

Authors+Show Affiliations

Stem Cell Lab, Department of Molecular Medicine, Sapienza University of Rome, Rome 00161, Italy.Stem Cell Lab, Department of Molecular Medicine, Sapienza University of Rome, Rome 00161, Italy.Stem Cell Lab, Department of Molecular Medicine, Sapienza University of Rome, Rome 00161, Italy.Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Sapienza University of Rome, Rome 00158, Italy.Institute for Transplant Diagnostics and Cellular Therapeutics, Medical Center Heinrich-Heine University, Duesseldorf 40225, Germany.Institute for Transplant Diagnostics and Cellular Therapeutics, Medical Center Heinrich-Heine University, Duesseldorf 40225, Germany.Institute of Inflammation and Repair, University of Manchester, Manchester M13 9PL, UK.Dulbecco Telethon Institute, Pediatric Department, Tettamanti Research Center, University of Milano-Bicocca, San Gerardo Hospital, Monza 20900, Italy.Department of Development and Regeneration, KU Leuven, Leuven 3000, Belgium.Center for Genome Research, University of Modena and Reggio Emilia, Modena 41121, Italy.Center for Genome Research, University of Modena and Reggio Emilia, Modena 41121, Italy.Department of Biology and Biotechnology "C. Darwin", Sapienza University, IBPM CNR, Rome 00185, Italy.Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Bethesda, MD 20892, USA. Electronic address: probey@dir.nidcr.nih.gov.Stem Cell Lab, Department of Molecular Medicine, Sapienza University of Rome, Rome 00161, Italy. Electronic address: mara.riminucci@uniroma1.it.Stem Cell Lab, Department of Molecular Medicine, Sapienza University of Rome, Rome 00161, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27304917

Citation

Sacchetti, Benedetto, et al. "No Identical "Mesenchymal Stem Cells" at Different Times and Sites: Human Committed Progenitors of Distinct Origin and Differentiation Potential Are Incorporated as Adventitial Cells in Microvessels." Stem Cell Reports, vol. 6, no. 6, 2016, pp. 897-913.
Sacchetti B, Funari A, Remoli C, et al. No Identical "Mesenchymal Stem Cells" at Different Times and Sites: Human Committed Progenitors of Distinct Origin and Differentiation Potential Are Incorporated as Adventitial Cells in Microvessels. Stem Cell Reports. 2016;6(6):897-913.
Sacchetti, B., Funari, A., Remoli, C., Giannicola, G., Kogler, G., Liedtke, S., ... Bianco, P. (2016). No Identical "Mesenchymal Stem Cells" at Different Times and Sites: Human Committed Progenitors of Distinct Origin and Differentiation Potential Are Incorporated as Adventitial Cells in Microvessels. Stem Cell Reports, 6(6), pp. 897-913. doi:10.1016/j.stemcr.2016.05.011.
Sacchetti B, et al. No Identical "Mesenchymal Stem Cells" at Different Times and Sites: Human Committed Progenitors of Distinct Origin and Differentiation Potential Are Incorporated as Adventitial Cells in Microvessels. Stem Cell Reports. 2016 06 14;6(6):897-913. PubMed PMID: 27304917.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - No Identical "Mesenchymal Stem Cells" at Different Times and Sites: Human Committed Progenitors of Distinct Origin and Differentiation Potential Are Incorporated as Adventitial Cells in Microvessels. AU - Sacchetti,Benedetto, AU - Funari,Alessia, AU - Remoli,Cristina, AU - Giannicola,Giuseppe, AU - Kogler,Gesine, AU - Liedtke,Stefanie, AU - Cossu,Giulio, AU - Serafini,Marta, AU - Sampaolesi,Maurilio, AU - Tagliafico,Enrico, AU - Tenedini,Elena, AU - Saggio,Isabella, AU - Robey,Pamela G, AU - Riminucci,Mara, AU - Bianco,Paolo, PY - 2015/07/10/received PY - 2016/05/20/revised PY - 2016/05/20/accepted PY - 2016/6/16/entrez PY - 2016/6/16/pubmed PY - 2017/11/29/medline KW - bone marrow stromal cell KW - differentiation KW - hematopoietic microenvironment KW - in vivo assays KW - mesenchymal stem cell KW - myogenic progenitors KW - skeletal progenitors KW - transplantation SP - 897 EP - 913 JF - Stem cell reports JO - Stem Cell Reports VL - 6 IS - 6 N2 - A widely shared view reads that mesenchymal stem/stromal cells ("MSCs") are ubiquitous in human connective tissues, can be defined by a common in vitro phenotype, share a skeletogenic potential as assessed by in vitro differentiation assays, and coincide with ubiquitous pericytes. Using stringent in vivo differentiation assays and transcriptome analysis, we show that human cell populations from different anatomical sources, regarded as "MSCs" based on these criteria and assumptions, actually differ widely in their transcriptomic signature and in vivo differentiation potential. In contrast, they share the capacity to guide the assembly of functional microvessels in vivo, regardless of their anatomical source, or in situ identity as perivascular or circulating cells. This analysis reveals that muscle pericytes, which are not spontaneously osteochondrogenic as previously claimed, may indeed coincide with an ectopic perivascular subset of committed myogenic cells similar to satellite cells. Cord blood-derived stromal cells, on the other hand, display the unique capacity to form cartilage in vivo spontaneously, in addition to an assayable osteogenic capacity. These data suggest the need to revise current misconceptions on the origin and function of so-called "MSCs," with important applicative implications. The data also support the view that rather than a uniform class of "MSCs," different mesoderm derivatives include distinct classes of tissue-specific committed progenitors, possibly of different developmental origin. SN - 2213-6711 UR - https://www.unboundmedicine.com/medline/citation/27304917/No_Identical_"Mesenchymal_Stem_Cells"_at_Different_Times_and_Sites:_Human_Committed_Progenitors_of_Distinct_Origin_and_Differentiation_Potential_Are_Incorporated_as_Adventitial_Cells_in_Microvessels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2213-6711(16)30066-2 DB - PRIME DP - Unbound Medicine ER -