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Characteristics of metal-tolerant plant growth-promoting yeast (Cryptococcus sp. NSE1) and its influence on Cd hyperaccumulator Sedum plumbizincicola.
Environ Sci Pollut Res Int. 2016 Sep; 23(18):18621-9.ES

Abstract

Plant growth-promoting yeasts are often over looked as a mechanism to improve phytoremediation of heavy metals. In this study, Cryptococcus sp. NSE1, a Cd-tolerant yeast with plant growth capabilities, was isolated from the rhizosphere of the heavy metal hyperaccumulator Sedum plumbizincicola. The yeast exhibited strong tolerance to a range of heavy metals including Cd, Cu, and Zn on plate assays. The adsorption rate Cd, Cu, Zn by NSE1 was 26.1, 13.2, and 25.2 %, respectively. Irregular spines were formed on the surface of NSE1 when grown in MSM medium supplemented with 200 mg L(-1) Cd. NSE1 was capable of utilizing 1-aminocyclopropane-1-carboxylate (ACC) as a sole nitrogen source and was capable of solubilization of inorganic phosphate at rates of 195.2 mg L(-1). Field experiments demonstrated that NSE1 increased phytoremediation by increasing the biomass of Cd hyperaccumulator S. plumbizincicola (46 %, p < 0.05) during phytoremediation. Overall, Cd accumulation by S. plumbizincicola was increased from 19.6 to 31.1 mg m(-2) though no difference in the concentration of Cd in the shoot biomass was observed between NSE1 and control. A Cd accumulation ratio of 38.0 % for NSE1 and 17.2 % for control was observed. The HCl-extractable Cd and CaCl2-extractable Cd concentration in the soil of the NSE1 treatment were reduced by 39.2 and 29.5 %, respectively. Community-level physiology profiling, assessed using Biolog Eco plates, indicated functional changes to the rhizosphere community inoculated with NSE1 by average well color development (AWCD) and measurement of richness (diversity). Values of Shannon-Weiner index, Simpson index, and McIntosh index showed a slight but no significant increases. These results indicate that inoculation of NSE1 could increase the shoot biomass of S. plumbizincicola, enhance the Cd accumulation in S. plumbizincicola, and decrease the available heavy metal content in soils significantly without overall significant changes to the microbial community.

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Authors+Show Affiliations

Liu W
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China. liuwuxin@issas.ac.cn.
Wang B
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China. University of Chinese Academy of Sciences, Beijing, 100049, China.
Wang Q
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.
Hou J
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China. University of Chinese Academy of Sciences, Beijing, 100049, China.
Wu L
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.
Wood JL
Department of Physiology, Anatomy and Microbiology, La Trobe University, Melbourne, VIC, 3086, Australia.
Luo Y
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China. Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
Franks AE
Department of Physiology, Anatomy and Microbiology, La Trobe University, Melbourne, VIC, 3086, Australia.

MeSH

Adaptation, PhysiologicalBiodegradation, EnvironmentalBiomassCadmiumCryptococcusMetals, HeavyPhosphatesPlant DevelopmentRhizosphereSaccharomyces cerevisiaeSedumSoilSoil PollutantsZinc

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27306207

Citation

Liu, Wuxing, et al. "Characteristics of Metal-tolerant Plant Growth-promoting Yeast (Cryptococcus Sp. NSE1) and Its Influence On Cd Hyperaccumulator Sedum Plumbizincicola." Environmental Science and Pollution Research International, vol. 23, no. 18, 2016, pp. 18621-9.
Liu W, Wang B, Wang Q, et al. Characteristics of metal-tolerant plant growth-promoting yeast (Cryptococcus sp. NSE1) and its influence on Cd hyperaccumulator Sedum plumbizincicola. Environ Sci Pollut Res Int. 2016;23(18):18621-9.
Liu, W., Wang, B., Wang, Q., Hou, J., Wu, L., Wood, J. L., Luo, Y., & Franks, A. E. (2016). Characteristics of metal-tolerant plant growth-promoting yeast (Cryptococcus sp. NSE1) and its influence on Cd hyperaccumulator Sedum plumbizincicola. Environmental Science and Pollution Research International, 23(18), 18621-9. https://doi.org/10.1007/s11356-016-7041-2
Liu W, et al. Characteristics of Metal-tolerant Plant Growth-promoting Yeast (Cryptococcus Sp. NSE1) and Its Influence On Cd Hyperaccumulator Sedum Plumbizincicola. Environ Sci Pollut Res Int. 2016;23(18):18621-9. PubMed PMID: 27306207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characteristics of metal-tolerant plant growth-promoting yeast (Cryptococcus sp. NSE1) and its influence on Cd hyperaccumulator Sedum plumbizincicola. AU - Liu,Wuxing, AU - Wang,Beibei, AU - Wang,Qingling, AU - Hou,Jinyu, AU - Wu,Longhua, AU - Wood,Jennifer L, AU - Luo,Yongming, AU - Franks,Ashley E, Y1 - 2016/06/15/ PY - 2016/02/20/received PY - 2016/06/05/accepted PY - 2016/6/17/entrez PY - 2016/6/17/pubmed PY - 2017/4/22/medline KW - Cd-contaminated soil KW - Cryptococcus sp. KW - Microbial community KW - Phytoremediation KW - Plant growth promoting SP - 18621 EP - 9 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 23 IS - 18 N2 - Plant growth-promoting yeasts are often over looked as a mechanism to improve phytoremediation of heavy metals. In this study, Cryptococcus sp. NSE1, a Cd-tolerant yeast with plant growth capabilities, was isolated from the rhizosphere of the heavy metal hyperaccumulator Sedum plumbizincicola. The yeast exhibited strong tolerance to a range of heavy metals including Cd, Cu, and Zn on plate assays. The adsorption rate Cd, Cu, Zn by NSE1 was 26.1, 13.2, and 25.2 %, respectively. Irregular spines were formed on the surface of NSE1 when grown in MSM medium supplemented with 200 mg L(-1) Cd. NSE1 was capable of utilizing 1-aminocyclopropane-1-carboxylate (ACC) as a sole nitrogen source and was capable of solubilization of inorganic phosphate at rates of 195.2 mg L(-1). Field experiments demonstrated that NSE1 increased phytoremediation by increasing the biomass of Cd hyperaccumulator S. plumbizincicola (46 %, p < 0.05) during phytoremediation. Overall, Cd accumulation by S. plumbizincicola was increased from 19.6 to 31.1 mg m(-2) though no difference in the concentration of Cd in the shoot biomass was observed between NSE1 and control. A Cd accumulation ratio of 38.0 % for NSE1 and 17.2 % for control was observed. The HCl-extractable Cd and CaCl2-extractable Cd concentration in the soil of the NSE1 treatment were reduced by 39.2 and 29.5 %, respectively. Community-level physiology profiling, assessed using Biolog Eco plates, indicated functional changes to the rhizosphere community inoculated with NSE1 by average well color development (AWCD) and measurement of richness (diversity). Values of Shannon-Weiner index, Simpson index, and McIntosh index showed a slight but no significant increases. These results indicate that inoculation of NSE1 could increase the shoot biomass of S. plumbizincicola, enhance the Cd accumulation in S. plumbizincicola, and decrease the available heavy metal content in soils significantly without overall significant changes to the microbial community. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/27306207/Characteristics_of_metal_tolerant_plant_growth_promoting_yeast__Cryptococcus_sp__NSE1__and_its_influence_on_Cd_hyperaccumulator_Sedum_plumbizincicola_ DB - PRIME DP - Unbound Medicine ER -