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Mixture toxicity of copper, cadmium, and zinc to barley seedlings is not explained by antioxidant and oxidative stress biomarkers.
Environ Toxicol Chem. 2017 01; 36(1):220-230.ET

Abstract

The analysis of metal mixture toxicity to plants is complicated by mutual interactions. In the present study, mixture effects of zinc (Zn), cadmium (Cd), and copper (Cu) on barley (Hordeum vulgare L.) root elongation were analyzed using oxidative stress parameters. The hypothesis was that toxic mixture effects on plant growth are better explained by biochemical parameters than by exposure information, because the former excludes interactions among metals for root uptake. Barley seedlings were exposed for 5 d or 14 d to these metals in nutrient solutions, added in isolation and as mixtures. Root elongation in Cu+Cd mixtures was well predicted from free metal ion concentrations in solution, using concentration addition (CA) or independent action (IA) reference models. In contrast, Zn acted antagonistically when combined with Cu and/or Cd, relative to both CA and IA. This protective effect of Zn correlated with the biomarkers measured in the long-term experiment; oxidative stress (indicated by malondialdehyde level, for example) decreased after addition of Zn. In addition, it was found that some biomarkers were sensitive to both Cu and Cd dosed in isolation, but not to Cu+Cd mixtures. Overall, the exposure explained mixture effects better than most of the 16 measured biomarkers (i.e., the biochemical effects). It is concluded that these biomarkers are not robust indicators for metal mixture toxicity, potentially because different metals have different parallel modes of action on growth that are insufficiently indexed by the biomarkers. Environ Toxicol Chem 2017;36:220-230. © 2016 SETAC.

Authors+Show Affiliations

Division of Soil and Water Management, KULeuven, Leuven, Belgium.Division of Soil and Water Management, KULeuven, Leuven, Belgium.Biology Department, University of Antwerp, Antwerp, Belgium.Biology Department, University of Antwerp, Antwerp, Belgium.Division of Soil and Water Management, KULeuven, Leuven, Belgium.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27311849

Citation

Versieren, Liske, et al. "Mixture Toxicity of Copper, Cadmium, and Zinc to Barley Seedlings Is Not Explained By Antioxidant and Oxidative Stress Biomarkers." Environmental Toxicology and Chemistry, vol. 36, no. 1, 2017, pp. 220-230.
Versieren L, Evers S, AbdElgawad H, et al. Mixture toxicity of copper, cadmium, and zinc to barley seedlings is not explained by antioxidant and oxidative stress biomarkers. Environ Toxicol Chem. 2017;36(1):220-230.
Versieren, L., Evers, S., AbdElgawad, H., Asard, H., & Smolders, E. (2017). Mixture toxicity of copper, cadmium, and zinc to barley seedlings is not explained by antioxidant and oxidative stress biomarkers. Environmental Toxicology and Chemistry, 36(1), 220-230. https://doi.org/10.1002/etc.3529
Versieren L, et al. Mixture Toxicity of Copper, Cadmium, and Zinc to Barley Seedlings Is Not Explained By Antioxidant and Oxidative Stress Biomarkers. Environ Toxicol Chem. 2017;36(1):220-230. PubMed PMID: 27311849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mixture toxicity of copper, cadmium, and zinc to barley seedlings is not explained by antioxidant and oxidative stress biomarkers. AU - Versieren,Liske, AU - Evers,Steffie, AU - AbdElgawad,Hamada, AU - Asard,Han, AU - Smolders,Erik, Y1 - 2016/07/26/ PY - 2016/01/21/received PY - 2016/03/15/revised PY - 2016/06/14/accepted PY - 2016/6/18/pubmed PY - 2017/7/18/medline PY - 2016/6/18/entrez KW - Biomarkers KW - Metals KW - Mixture toxicity KW - Oxidative stress KW - Plants SP - 220 EP - 230 JF - Environmental toxicology and chemistry JO - Environ Toxicol Chem VL - 36 IS - 1 N2 - The analysis of metal mixture toxicity to plants is complicated by mutual interactions. In the present study, mixture effects of zinc (Zn), cadmium (Cd), and copper (Cu) on barley (Hordeum vulgare L.) root elongation were analyzed using oxidative stress parameters. The hypothesis was that toxic mixture effects on plant growth are better explained by biochemical parameters than by exposure information, because the former excludes interactions among metals for root uptake. Barley seedlings were exposed for 5 d or 14 d to these metals in nutrient solutions, added in isolation and as mixtures. Root elongation in Cu+Cd mixtures was well predicted from free metal ion concentrations in solution, using concentration addition (CA) or independent action (IA) reference models. In contrast, Zn acted antagonistically when combined with Cu and/or Cd, relative to both CA and IA. This protective effect of Zn correlated with the biomarkers measured in the long-term experiment; oxidative stress (indicated by malondialdehyde level, for example) decreased after addition of Zn. In addition, it was found that some biomarkers were sensitive to both Cu and Cd dosed in isolation, but not to Cu+Cd mixtures. Overall, the exposure explained mixture effects better than most of the 16 measured biomarkers (i.e., the biochemical effects). It is concluded that these biomarkers are not robust indicators for metal mixture toxicity, potentially because different metals have different parallel modes of action on growth that are insufficiently indexed by the biomarkers. Environ Toxicol Chem 2017;36:220-230. © 2016 SETAC. SN - 1552-8618 UR - https://www.unboundmedicine.com/medline/citation/27311849/Mixture_toxicity_of_copper_cadmium_and_zinc_to_barley_seedlings_is_not_explained_by_antioxidant_and_oxidative_stress_biomarkers_ DB - PRIME DP - Unbound Medicine ER -