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Isolation and Characterization of Anthocyanins from Hibiscus sabdariffa Flowers.
J Nat Prod. 2016 07 22; 79(7):1709-18.JN

Abstract

The intense red-colored Hibiscus sabdariffa flowers are an inexpensive source of anthocyanins with potential to be used as natural, innocuous, and health-beneficial colorants. An anthocyanin-rich extract from hibiscus flowers was obtained by ultrasound-assisted extraction. By a single-step process fractionation using a Sep-Pak C18 cartridge, the main hibiscus anthocyanins, delphinidin-3-O-sambubioside (Dp-samb) and cyanidin-3-O-sambubioside (Cy-samb), were separated and then characterized via NMR and HPLC-ESIMS data. Since Dp-samb was the most abundant anthocyanin identified in the extract, its colorant properties were studied by the pH jumps method, which allowed the calculation of the single acid-base equilibrium (pK'a 2.92), the acidity (pKa 3.70), and the hydration constants (pKh 3.02). Moreover, by using size-exclusion chromatography, new cyanidin-derived anthocyanins (with three or more sugar units) were successfully identified and reported for the first time in the hibiscus extract.

Authors+Show Affiliations

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableREQUIMTE - Laboratório Associado para a Química Verde, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto , Rua do Campo Alegre, 687, 4169-007 Porto, Portugal.REQUIMTE - Laboratório Associado para a Química Verde, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto , Rua do Campo Alegre, 687, 4169-007 Porto, Portugal.Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua , Circuito Universitario s/n, Campus Universitario No. 2, CP 31125, Chihuahua, México.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27312226

Citation

Grajeda-Iglesias, Claudia, et al. "Isolation and Characterization of Anthocyanins From Hibiscus Sabdariffa Flowers." Journal of Natural Products, vol. 79, no. 7, 2016, pp. 1709-18.
Grajeda-Iglesias C, Figueroa-Espinoza MC, Barouh N, et al. Isolation and Characterization of Anthocyanins from Hibiscus sabdariffa Flowers. J Nat Prod. 2016;79(7):1709-18.
Grajeda-Iglesias, C., Figueroa-Espinoza, M. C., Barouh, N., Baréa, B., Fernandes, A., de Freitas, V., & Salas, E. (2016). Isolation and Characterization of Anthocyanins from Hibiscus sabdariffa Flowers. Journal of Natural Products, 79(7), 1709-18. https://doi.org/10.1021/acs.jnatprod.5b00958
Grajeda-Iglesias C, et al. Isolation and Characterization of Anthocyanins From Hibiscus Sabdariffa Flowers. J Nat Prod. 2016 07 22;79(7):1709-18. PubMed PMID: 27312226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and Characterization of Anthocyanins from Hibiscus sabdariffa Flowers. AU - Grajeda-Iglesias,Claudia, AU - Figueroa-Espinoza,Maria C, AU - Barouh,Nathalie, AU - Baréa,Bruno, AU - Fernandes,Ana, AU - de Freitas,Victor, AU - Salas,Erika, Y1 - 2016/06/17/ PY - 2016/6/18/entrez PY - 2016/6/18/pubmed PY - 2017/5/11/medline SP - 1709 EP - 18 JF - Journal of natural products JO - J Nat Prod VL - 79 IS - 7 N2 - The intense red-colored Hibiscus sabdariffa flowers are an inexpensive source of anthocyanins with potential to be used as natural, innocuous, and health-beneficial colorants. An anthocyanin-rich extract from hibiscus flowers was obtained by ultrasound-assisted extraction. By a single-step process fractionation using a Sep-Pak C18 cartridge, the main hibiscus anthocyanins, delphinidin-3-O-sambubioside (Dp-samb) and cyanidin-3-O-sambubioside (Cy-samb), were separated and then characterized via NMR and HPLC-ESIMS data. Since Dp-samb was the most abundant anthocyanin identified in the extract, its colorant properties were studied by the pH jumps method, which allowed the calculation of the single acid-base equilibrium (pK'a 2.92), the acidity (pKa 3.70), and the hydration constants (pKh 3.02). Moreover, by using size-exclusion chromatography, new cyanidin-derived anthocyanins (with three or more sugar units) were successfully identified and reported for the first time in the hibiscus extract. SN - 1520-6025 UR - https://www.unboundmedicine.com/medline/citation/27312226/Isolation_and_Characterization_of_Anthocyanins_from_Hibiscus_sabdariffa_Flowers_ L2 - https://doi.org/10.1021/acs.jnatprod.5b00958 DB - PRIME DP - Unbound Medicine ER -