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Rapid Detection and Characterisation of Triterpene Saponins from the Root of Pulsatilla chinensis (Bunge) Regel by HPLC-ESI-QTOF-MS/MS.
Phytochem Anal. 2016 May; 27(3-4):174-83.PA

Abstract

INTRODUCTION

Triterpene saponins are the major bioactive components in the root of Pulsatilla chinensis (Bunge) Regel (RPC), and have been reported to possess antitumor and immunological adjuvant activities. However, the isolation, purification and elucidation procedures of triterpene saponins from RPC are difficult and time consuming due to high polarity and structural similarity.

OBJECTIVES

To develop an analytical strategy for discovering and elucidating triterpene saponins in RPC.

METHODS

Methanolic extract of RPC is analysed by high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight-mass spectrometry (HPLC-ESI-QTOF-MS/MS). The MS and MS/MS experiments are conducted using the negative-ionisation mode, in order to provide molecular-mass information and production spectra for the structural elucidation of compounds.

RESULTS

Based on retention times, accurate mass and mass spectrometric fragmentation, 24 triterpene saponins are identified or tentatively elucidated from RPC, of which nine triterpene saponins were not reported previously.

CONCLUSION

The HPLC-ESI-QTOF-MS/MS could be employed as a rapid, effective technique to screen and identify triterpene saponins in RPC without tedious and time-consuming isolation of pure constituents. Copyright © 2016 John Wiley & Sons, Ltd.

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Authors+Show Affiliations

Ling Y
Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang, 332005, People's Republic of China.
Lin Z
Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang, 332005, People's Republic of China.
Zha W
Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang, 332005, People's Republic of China.
Lian T
Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang, 332005, People's Republic of China.
You S
No affiliation info available

MeSH

Chromatography, High Pressure LiquidMethanolMolecular StructurePlant ExtractsPlant RootsPulsatillaSaponinsSpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryTime FactorsTriterpenes

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27313154

Citation

Ling, Yun, et al. "Rapid Detection and Characterisation of Triterpene Saponins From the Root of Pulsatilla Chinensis (Bunge) Regel By HPLC-ESI-QTOF-MS/MS." Phytochemical Analysis : PCA, vol. 27, no. 3-4, 2016, pp. 174-83.
Ling Y, Lin Z, Zha W, et al. Rapid Detection and Characterisation of Triterpene Saponins from the Root of Pulsatilla chinensis (Bunge) Regel by HPLC-ESI-QTOF-MS/MS. Phytochem Anal. 2016;27(3-4):174-83.
Ling, Y., Lin, Z., Zha, W., Lian, T., & You, S. (2016). Rapid Detection and Characterisation of Triterpene Saponins from the Root of Pulsatilla chinensis (Bunge) Regel by HPLC-ESI-QTOF-MS/MS. Phytochemical Analysis : PCA, 27(3-4), 174-83. https://doi.org/10.1002/pca.2613
Ling Y, et al. Rapid Detection and Characterisation of Triterpene Saponins From the Root of Pulsatilla Chinensis (Bunge) Regel By HPLC-ESI-QTOF-MS/MS. Phytochem Anal. 2016;27(3-4):174-83. PubMed PMID: 27313154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid Detection and Characterisation of Triterpene Saponins from the Root of Pulsatilla chinensis (Bunge) Regel by HPLC-ESI-QTOF-MS/MS. AU - Ling,Yun, AU - Lin,Zhanqing, AU - Zha,Wenjuan, AU - Lian,Tou, AU - You,Shanshan, PY - 2015/10/22/received PY - 2016/01/08/revised PY - 2016/01/09/accepted PY - 2016/6/18/entrez PY - 2016/6/18/pubmed PY - 2017/5/17/medline KW - HPLC-ESI-QTOF-MS/MS KW - Pulsatilla chinensis (Bunge) Regel KW - triterpene saponins SP - 174 EP - 83 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 27 IS - 3-4 N2 - INTRODUCTION: Triterpene saponins are the major bioactive components in the root of Pulsatilla chinensis (Bunge) Regel (RPC), and have been reported to possess antitumor and immunological adjuvant activities. However, the isolation, purification and elucidation procedures of triterpene saponins from RPC are difficult and time consuming due to high polarity and structural similarity. OBJECTIVES: To develop an analytical strategy for discovering and elucidating triterpene saponins in RPC. METHODS: Methanolic extract of RPC is analysed by high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight-mass spectrometry (HPLC-ESI-QTOF-MS/MS). The MS and MS/MS experiments are conducted using the negative-ionisation mode, in order to provide molecular-mass information and production spectra for the structural elucidation of compounds. RESULTS: Based on retention times, accurate mass and mass spectrometric fragmentation, 24 triterpene saponins are identified or tentatively elucidated from RPC, of which nine triterpene saponins were not reported previously. CONCLUSION: The HPLC-ESI-QTOF-MS/MS could be employed as a rapid, effective technique to screen and identify triterpene saponins in RPC without tedious and time-consuming isolation of pure constituents. Copyright © 2016 John Wiley & Sons, Ltd. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/27313154/Rapid_Detection_and_Characterisation_of_Triterpene_Saponins_from_the_Root_of_Pulsatilla_chinensis__Bunge__Regel_by_HPLC_ESI_QTOF_MS/MS_ DB - PRIME DP - Unbound Medicine ER -
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