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Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni) Juice Treatment.
Evid Based Complement Alternat Med. 2016; 2016:5063540.EB

Abstract

The search for new treatments against leishmaniasis has increased due to high frequency of drug resistance registered in endemics areas, side effects, and complications caused by coinfection with HIV. Morinda citrifolia Linn., commonly known as Noni, has a rich chemical composition and various therapeutic effects have been described in the literature. Studies have shown the leishmanicidal activity of M. citrifolia; however, its action on the parasite has not yet been elucidated. In this work, we analyzed leishmanicidal activity and ultrastructural changes in Leishmania infantum promastigotes caused by M. citrifolia fruit juice treatment. M. citrifolia fruit extract showed a yield of 6.31% and high performance liquid chromatography identified phenolic and aromatic compounds as the major constituents. IC50 values were 260.5 µg/mL for promastigotes and 201.3 µg/mL for intracellular amastigotes of L. infantum treated with M. citrifolia. Cytotoxicity assay with J774.G8 macrophages showed that M. citrifolia fruit juice was not toxic up to 2 mg/mL. Transmission electron microscopy showed cytoplasmic vacuolization, lipid inclusion, increased exocytosis activity, and autophagosome-like vesicles in L. infantum promastigotes treated with M. citrifolia fruit juice. M. citrifolia fruit juice was active against L. infantum in the in vitro model used here causing ultrastructural changes and has a future potential for treatment against leishmaniasis.

Authors+Show Affiliations

Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, Fiocruz, 21040-900 Rio de Janeiro, RJ, Brazil; Departamento de Patologia, Universidade Estadual do Maranhão, 65055-310 São Luís, MA, Brazil.Unidade de Microscopia Eletrônica, Instituto Adolf Lutz, 01246-000 São Paulo, SP, Brazil.Laboratório de Plantas Medicinais e Derivados, Farmanguinhos, Fiocruz, 21041-250 Rio de Janeiro, RJ, Brazil.Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, Fiocruz, 21040-900 Rio de Janeiro, RJ, Brazil.Laboratório de Imunomodulação e Protozoologia, Instituto Oswaldo Cruz, Fiocruz, 21040-900 Rio de Janeiro, RJ, Brazil.Departamento de Patologia, Universidade Estadual do Maranhão, 65055-310 São Luís, MA, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27313649

Citation

Almeida-Souza, Fernando, et al. "Ultrastructural Changes and Death of Leishmania Infantum Promastigotes Induced By Morinda Citrifolia Linn. Fruit (Noni) Juice Treatment." Evidence-based Complementary and Alternative Medicine : ECAM, vol. 2016, 2016, p. 5063540.
Almeida-Souza F, Taniwaki NN, Amaral AC, et al. Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni) Juice Treatment. Evid Based Complement Alternat Med. 2016;2016:5063540.
Almeida-Souza, F., Taniwaki, N. N., Amaral, A. C., de Souza, C. d. a. . S., Calabrese, K. d. a. . S., & Abreu-Silva, A. L. (2016). Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni) Juice Treatment. Evidence-based Complementary and Alternative Medicine : ECAM, 2016, 5063540. https://doi.org/10.1155/2016/5063540
Almeida-Souza F, et al. Ultrastructural Changes and Death of Leishmania Infantum Promastigotes Induced By Morinda Citrifolia Linn. Fruit (Noni) Juice Treatment. Evid Based Complement Alternat Med. 2016;2016:5063540. PubMed PMID: 27313649.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni) Juice Treatment. AU - Almeida-Souza,Fernando, AU - Taniwaki,Noemi Nosomi, AU - Amaral,Ana Cláudia Fernandes, AU - de Souza,Celeste da Silva Freitas, AU - Calabrese,Kátia da Silva, AU - Abreu-Silva,Ana Lúcia, Y1 - 2016/05/22/ PY - 2016/01/22/received PY - 2016/03/23/accepted PY - 2016/6/18/entrez PY - 2016/6/18/pubmed PY - 2016/6/18/medline SP - 5063540 EP - 5063540 JF - Evidence-based complementary and alternative medicine : eCAM JO - Evid Based Complement Alternat Med VL - 2016 N2 - The search for new treatments against leishmaniasis has increased due to high frequency of drug resistance registered in endemics areas, side effects, and complications caused by coinfection with HIV. Morinda citrifolia Linn., commonly known as Noni, has a rich chemical composition and various therapeutic effects have been described in the literature. Studies have shown the leishmanicidal activity of M. citrifolia; however, its action on the parasite has not yet been elucidated. In this work, we analyzed leishmanicidal activity and ultrastructural changes in Leishmania infantum promastigotes caused by M. citrifolia fruit juice treatment. M. citrifolia fruit extract showed a yield of 6.31% and high performance liquid chromatography identified phenolic and aromatic compounds as the major constituents. IC50 values were 260.5 µg/mL for promastigotes and 201.3 µg/mL for intracellular amastigotes of L. infantum treated with M. citrifolia. Cytotoxicity assay with J774.G8 macrophages showed that M. citrifolia fruit juice was not toxic up to 2 mg/mL. Transmission electron microscopy showed cytoplasmic vacuolization, lipid inclusion, increased exocytosis activity, and autophagosome-like vesicles in L. infantum promastigotes treated with M. citrifolia fruit juice. M. citrifolia fruit juice was active against L. infantum in the in vitro model used here causing ultrastructural changes and has a future potential for treatment against leishmaniasis. SN - 1741-427X UR - https://www.unboundmedicine.com/medline/citation/27313649/Ultrastructural_Changes_and_Death_of_Leishmania_infantum_Promastigotes_Induced_by_Morinda_citrifolia_Linn__Fruit__Noni__Juice_Treatment_ L2 - https://dx.doi.org/10.1155/2016/5063540 DB - PRIME DP - Unbound Medicine ER -
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