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Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase-Producing Escherichia coli ST410 in the United Kingdom.
Microb Drug Resist. 2016 Oct; 22(7):609-615.MD

Abstract

We characterized extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) in 32 Escherichia coli extended spectrum cephalosporin (ESC)-resistant clinical isolates from UK companion animals from several clinics. In addition, to investigate the possible dissemination of ESBL clinical isolates within a veterinary hospital, two ESBL-producing E. coli isolates from a dog with septic peritonitis and a cluster of environmental ESC-resistant E. coli isolates obtained from the same clinic and during the same time period, as these two particular ESBL-positive clinical isolates, were also included in the study. Molecular characterization identified blaCTX-M to be the most prevalent gene in ESC-resistant isolates, where 66% and 27% of clinical isolates carried blaCTX-M-15 and blaCTX-M-14, respectively. The only PMQR gene detected was aac(6')-Ib-cr, being found in 34% of the ESC E. coli isolates and was associated with the carriage of blaCTX-M-15. The clinical and environmental isolates investigated for hospital dissemination had a common ESBL/AmpC phenotype, carried blaCTX-M-15, and co-harbored blaOXA-1, blaTEM-1, blaCMY-2, and aac(6')-Ib-cr. Multilocus sequence typing identified them all as ST410, while pulse-field gel electrophoresis demonstrated 100% homology of clinical and environmental isolates, suggesting hospital environmental dissemination of CTX-M-15-producing E. coli ST410.

Authors+Show Affiliations

1 School of Veterinary Science, University of Liverpool , Liverpool, United Kingdom . 2 Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool , Liverpool, United Kingdom . 3 Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine , Iasi, Romania .1 School of Veterinary Science, University of Liverpool , Liverpool, United Kingdom .4 Department of Epidemiology and Population Health, Institute of Infection and Global Health, University of Liverpool , Liverpool, United Kingdom .1 School of Veterinary Science, University of Liverpool , Liverpool, United Kingdom .1 School of Veterinary Science, University of Liverpool , Liverpool, United Kingdom . 2 Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool , Liverpool, United Kingdom .

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27314838

Citation

Timofte, Dorina, et al. "Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase-Producing Escherichia Coli ST410 in the United Kingdom." Microbial Drug Resistance (Larchmont, N.Y.), vol. 22, no. 7, 2016, pp. 609-615.
Timofte D, Maciuca IE, Williams NJ, et al. Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase-Producing Escherichia coli ST410 in the United Kingdom. Microb Drug Resist. 2016;22(7):609-615.
Timofte, D., Maciuca, I. E., Williams, N. J., Wattret, A., & Schmidt, V. (2016). Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase-Producing Escherichia coli ST410 in the United Kingdom. Microbial Drug Resistance (Larchmont, N.Y.), 22(7), 609-615.
Timofte D, et al. Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase-Producing Escherichia Coli ST410 in the United Kingdom. Microb Drug Resist. 2016;22(7):609-615. PubMed PMID: 27314838.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase-Producing Escherichia coli ST410 in the United Kingdom. AU - Timofte,Dorina, AU - Maciuca,Iuliana Elena, AU - Williams,Nicola J, AU - Wattret,Andrew, AU - Schmidt,Vanessa, Y1 - 2016/06/17/ PY - 2016/6/18/pubmed PY - 2017/4/20/medline PY - 2016/6/18/entrez KW - E. coli KW - ESBL KW - infection control KW - surveillance KW - veterinary SP - 609 EP - 615 JF - Microbial drug resistance (Larchmont, N.Y.) JO - Microb Drug Resist VL - 22 IS - 7 N2 - We characterized extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) in 32 Escherichia coli extended spectrum cephalosporin (ESC)-resistant clinical isolates from UK companion animals from several clinics. In addition, to investigate the possible dissemination of ESBL clinical isolates within a veterinary hospital, two ESBL-producing E. coli isolates from a dog with septic peritonitis and a cluster of environmental ESC-resistant E. coli isolates obtained from the same clinic and during the same time period, as these two particular ESBL-positive clinical isolates, were also included in the study. Molecular characterization identified blaCTX-M to be the most prevalent gene in ESC-resistant isolates, where 66% and 27% of clinical isolates carried blaCTX-M-15 and blaCTX-M-14, respectively. The only PMQR gene detected was aac(6')-Ib-cr, being found in 34% of the ESC E. coli isolates and was associated with the carriage of blaCTX-M-15. The clinical and environmental isolates investigated for hospital dissemination had a common ESBL/AmpC phenotype, carried blaCTX-M-15, and co-harbored blaOXA-1, blaTEM-1, blaCMY-2, and aac(6')-Ib-cr. Multilocus sequence typing identified them all as ST410, while pulse-field gel electrophoresis demonstrated 100% homology of clinical and environmental isolates, suggesting hospital environmental dissemination of CTX-M-15-producing E. coli ST410. SN - 1931-8448 UR - https://www.unboundmedicine.com/medline/citation/27314838/Veterinary_Hospital_Dissemination_of_CTX_M_15_Extended_Spectrum_Beta_Lactamase_Producing_Escherichia_coli_ST410_in_the_United_Kingdom_ L2 - https://www.liebertpub.com/doi/10.1089/mdr.2016.0036?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -