Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction.
J Chromatogr A. 2016 Jul 22; 1456:1-9.JC

Abstract

Molecularly imprinted polymer nanoparticles (MIPNPs) with the ability to recognize coenzyme Q10 (CoQ10) were synthesised in order to be employed as sorbent in a dispersive micro-solid phase extraction (DMSPE) for the determination of CoQ10 in a liver extract. CoQ10 is a redox-active, lipophilic substance integrated in the mitochondrial respiratory chain which acts as an electron carrier, shuttling electrons from complex I (NADH-ubiquinone oxidoreductase) and II (succinate-ubiquinone oxidoreductase) to complex III (ubiquinol-cytochrome c reductase), for the production of cellular energy. The MIPNPs were synthesised by precipitation polymerization using coenzyme Q0 as the dummy template, methacrylic acid as the functional monomer, an acetonitrile: water mixture as the porogen, ethylene glycol dimethacrylate as the crosslinker and potassium persulfate as initiator. The nanoparticles were characterized by microscopy, capillary electrophoresis, dynamic light scattering, N2 adsorption-desorption isotherms, and infrared spectroscopy. The MIPNPs demonstrated the presence of selective cavities complementary to the quinone nucleus of CoQ10, leading to a specific recognition of CoQ10 compared with related compounds. In the liver extract the relative CoQ10 peak area (CoQ10 area/total peak area) increased from 4.6% to 25.4% after the DMSPE procedure. The recovery percentage of CoQ10 from the liver matrix was between 70.5% and 83.7% quantified against CoQ10 standard processed under the same conditions. The DMSPE procedure allows the elution of almost all the CoQ10 retained (99.4%) in a small volume (200μL), allowing the sample to be concentrated 2.5 times (LOD: 1.1μgg(-1) and LOQ: 3.7μgg(-1) of tissue). The resulted clean up of the sample, the improvement in peak shape and baseline and the reduction of interferences, evidence that the MIPNPs could potentially be applied as sorbent in a DMSPE with satisfactory results and with a minimum amount of sorbent (1mg).

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Authors+Show Affiliations

Contin M

Departamento de Tecnología Farmacéutica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, C1113AAD Buenos Aires, Argentina.

Bonelli P

PINMATE-Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428EGA Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina.

Lucangioli S

Departamento de Tecnología Farmacéutica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, C1113AAD Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas, CONICET, Buenos Aires, Argentina.

Cukierman A

Tripodi V

MeSH

AdsorptionAnimalsCattleCross-Linking ReagentsLiverMethacrylatesMolecular ImprintingNanoparticlesPolymerizationPolymethacrylic AcidsSolid Phase ExtractionUbiquinone

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27317007

Citation

Contin, Mario, et al. "Synthesis and Characterization of Molecularly Imprinted Polymer Nanoparticles for Coenzyme Q10 Dispersive Micro Solid Phase Extraction." Journal of Chromatography. A, vol. 1456, 2016, pp. 1-9.

Contin M, Bonelli P, Lucangioli S, et al. Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction. J Chromatogr A. 2016;1456:1-9.

Contin, M., Bonelli, P., Lucangioli, S., Cukierman, A., & Tripodi, V. (2016). Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction. Journal of Chromatography. A, 1456, 1-9. https://doi.org/10.1016/j.chroma.2016.05.091

Contin M, et al. Synthesis and Characterization of Molecularly Imprinted Polymer Nanoparticles for Coenzyme Q10 Dispersive Micro Solid Phase Extraction. J Chromatogr A. 2016 Jul 22;1456:1-9. PubMed PMID: 27317007.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction. AU - Contin,Mario, AU - Bonelli,Pablo, AU - Lucangioli,Silvia, AU - Cukierman,Ana, AU - Tripodi,Valeria, Y1 - 2016/05/27/ PY - 2015/11/16/received PY - 2016/05/23/revised PY - 2016/05/26/accepted PY - 2016/6/19/entrez PY - 2016/6/19/pubmed PY - 2016/12/15/medline KW - Coenzyme Q10 KW - Dispersive micro solid phase extraction KW - Molecularly imprinted polymer nanoparticles SP - 1 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1456 N2 - Molecularly imprinted polymer nanoparticles (MIPNPs) with the ability to recognize coenzyme Q10 (CoQ10) were synthesised in order to be employed as sorbent in a dispersive micro-solid phase extraction (DMSPE) for the determination of CoQ10 in a liver extract. CoQ10 is a redox-active, lipophilic substance integrated in the mitochondrial respiratory chain which acts as an electron carrier, shuttling electrons from complex I (NADH-ubiquinone oxidoreductase) and II (succinate-ubiquinone oxidoreductase) to complex III (ubiquinol-cytochrome c reductase), for the production of cellular energy. The MIPNPs were synthesised by precipitation polymerization using coenzyme Q0 as the dummy template, methacrylic acid as the functional monomer, an acetonitrile: water mixture as the porogen, ethylene glycol dimethacrylate as the crosslinker and potassium persulfate as initiator. The nanoparticles were characterized by microscopy, capillary electrophoresis, dynamic light scattering, N2 adsorption-desorption isotherms, and infrared spectroscopy. The MIPNPs demonstrated the presence of selective cavities complementary to the quinone nucleus of CoQ10, leading to a specific recognition of CoQ10 compared with related compounds. In the liver extract the relative CoQ10 peak area (CoQ10 area/total peak area) increased from 4.6% to 25.4% after the DMSPE procedure. The recovery percentage of CoQ10 from the liver matrix was between 70.5% and 83.7% quantified against CoQ10 standard processed under the same conditions. The DMSPE procedure allows the elution of almost all the CoQ10 retained (99.4%) in a small volume (200μL), allowing the sample to be concentrated 2.5 times (LOD: 1.1μgg(-1) and LOQ: 3.7μgg(-1) of tissue). The resulted clean up of the sample, the improvement in peak shape and baseline and the reduction of interferences, evidence that the MIPNPs could potentially be applied as sorbent in a DMSPE with satisfactory results and with a minimum amount of sorbent (1mg). SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/27317007/Synthesis_and_characterization_of_molecularly_imprinted_polymer_nanoparticles_for_coenzyme_Q10_dispersive_micro_solid_phase_extraction_ DB - PRIME DP - Unbound Medicine ER -

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Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction.J Chromatogr A. 2016 Jul 22; 1456:1-9.JC