Tags

Type your tag names separated by a space and hit enter

Reduced Levels of Tissue Inhibitors of Metalloproteinases in UVB-Irradiated Corneal Epithelium.
Photochem Photobiol. 2016 09; 92(5):720-7.PP

Abstract

Tissue inhibitors of metalloproteinases (TIMPs) are the major endogenous regulators of metalloproteinase activity in tissues. TIMPs are able to inhibit activity of all known matrix metalloproteinases (MMPs) and thus participate in controlling extracellular matrix synthesis and degradation. We showed previously elevated expressions of MMPs in the rabbit corneal epithelium upon UVB exposure and suggested that these enzymes might be involved in corneal destruction caused by excessive proteolysis. The aim of this study was to investigate TIMPs in the corneal epithelium after UV irradiation using immunohistochemical and biochemical methods. We found that as compared to control rabbit corneas where relatively high levels of TIMPs were present in the epithelium, repeated irradiation of the cornea with UVB rays (not with UVA rays of similar doses) significantly decreased TIMPs in corneal epithelial cells. The results of this study point to the suggestion that the decrease in TIMPs in the corneal epithelium after UVB irradiation contributes to increased proteolytic activity of MMPs in UVB-irradiated corneal epithelium found previously.

Authors+Show Affiliations

Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic. ardan@iapg.cas.cz.Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic.Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic.Faculty Hospital Královské Vinohrady, Prague, Czech Republic.Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic.Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27317620

Citation

Ardan, Taras, et al. "Reduced Levels of Tissue Inhibitors of Metalloproteinases in UVB-Irradiated Corneal Epithelium." Photochemistry and Photobiology, vol. 92, no. 5, 2016, pp. 720-7.
Ardan T, Němcová L, Bohuslavová B, et al. Reduced Levels of Tissue Inhibitors of Metalloproteinases in UVB-Irradiated Corneal Epithelium. Photochem Photobiol. 2016;92(5):720-7.
Ardan, T., Němcová, L., Bohuslavová, B., Klezlová, A., Popelka, Š., Studenovská, H., Hrnčiarová, E., Čejková, J., & Motlík, J. (2016). Reduced Levels of Tissue Inhibitors of Metalloproteinases in UVB-Irradiated Corneal Epithelium. Photochemistry and Photobiology, 92(5), 720-7. https://doi.org/10.1111/php.12612
Ardan T, et al. Reduced Levels of Tissue Inhibitors of Metalloproteinases in UVB-Irradiated Corneal Epithelium. Photochem Photobiol. 2016;92(5):720-7. PubMed PMID: 27317620.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reduced Levels of Tissue Inhibitors of Metalloproteinases in UVB-Irradiated Corneal Epithelium. AU - Ardan,Taras, AU - Němcová,Lucie, AU - Bohuslavová,Božena, AU - Klezlová,Adéla, AU - Popelka,Štěpán, AU - Studenovská,Hana, AU - Hrnčiarová,Eva, AU - Čejková,Jitka, AU - Motlík,Jan, Y1 - 2016/08/25/ PY - 2016/03/07/received PY - 2016/06/06/accepted PY - 2016/6/19/entrez PY - 2016/6/19/pubmed PY - 2018/11/8/medline SP - 720 EP - 7 JF - Photochemistry and photobiology JO - Photochem Photobiol VL - 92 IS - 5 N2 - Tissue inhibitors of metalloproteinases (TIMPs) are the major endogenous regulators of metalloproteinase activity in tissues. TIMPs are able to inhibit activity of all known matrix metalloproteinases (MMPs) and thus participate in controlling extracellular matrix synthesis and degradation. We showed previously elevated expressions of MMPs in the rabbit corneal epithelium upon UVB exposure and suggested that these enzymes might be involved in corneal destruction caused by excessive proteolysis. The aim of this study was to investigate TIMPs in the corneal epithelium after UV irradiation using immunohistochemical and biochemical methods. We found that as compared to control rabbit corneas where relatively high levels of TIMPs were present in the epithelium, repeated irradiation of the cornea with UVB rays (not with UVA rays of similar doses) significantly decreased TIMPs in corneal epithelial cells. The results of this study point to the suggestion that the decrease in TIMPs in the corneal epithelium after UVB irradiation contributes to increased proteolytic activity of MMPs in UVB-irradiated corneal epithelium found previously. SN - 1751-1097 UR - https://www.unboundmedicine.com/medline/citation/27317620/Reduced_Levels_of_Tissue_Inhibitors_of_Metalloproteinases_in_UVB_Irradiated_Corneal_Epithelium_ L2 - https://doi.org/10.1111/php.12612 DB - PRIME DP - Unbound Medicine ER -