Tags

Type your tag names separated by a space and hit enter

Distinct immune tones are established by Lactococcus lactis BFE920 and Lactobacillus plantarum FGL0001 in the gut of olive flounder (Paralichthys olivaceus).
Fish Shellfish Immunol. 2016 Aug; 55:434-43.FS

Abstract

The immune tone is defined as an immunological state during which the readiness for immune response is potentiated. The establishment of immune tone in the gut of olive flounder (Paralichthys olivaceus) was investigated by feeding Lactococcus lactis BFE920 (LL) or Lactobacillus plantarum FGL0001 (LP). LL-fed flounder showed significantly increased levels of regulatory genes (FOXP3, IL-10, and TGF-β1), CD18, and CD83 in the gut. In contrast, LP feeding drastically increased proinflammatory genes (T-bet, IL-1β, and IFN-γ) and CD18. This indicates that LL and LP establish different types of local immune tones in the gut through differential activation of innate immune cells: LL activates both macrophages and dendritic cells while LP activates macrophages only. Both of the immune tones required at least a total of 6 probiotic feeds during 72 h for a stable establishment. Once established, the type of immune tone remained steady even up to 30 days (a total of 60 feeds) probiotics feeding. The LL-induced regulatory immune tone enhanced the level of occludin, a tight junction molecule, significantly more than that observed with the proinflammatory immune tone established by LP feeding. Consequently, LL-fed fish showed considerably lower gut permeability than that of the LP-fed group. Furthermore, when orally challenged by Edwardsiella tarda, LL-fed flounder survived at a significantly higher rate than LP-fed fish. The data clearly demonstrate that individual probiotics establish distinct types of immune tone in the fish gut, which in turn influences the immunological status as well as the physiology of the gut. Selection of proper probiotics may be essential for optimal effects in aquaculture farming.

Authors+Show Affiliations

School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea.Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea.School of Life Science, Handong University, Pohang 791-708, Republic of Korea. Electronic address: sksong@handong.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27320869

Citation

Beck, Bo Ram, et al. "Distinct Immune Tones Are Established By Lactococcus Lactis BFE920 and Lactobacillus Plantarum FGL0001 in the Gut of Olive Flounder (Paralichthys Olivaceus)." Fish & Shellfish Immunology, vol. 55, 2016, pp. 434-43.
Beck BR, Song JH, Park BS, et al. Distinct immune tones are established by Lactococcus lactis BFE920 and Lactobacillus plantarum FGL0001 in the gut of olive flounder (Paralichthys olivaceus). Fish Shellfish Immunol. 2016;55:434-43.
Beck, B. R., Song, J. H., Park, B. S., Kim, D., Kwak, J. H., Do, H. K., Kim, A. R., Kim, W. J., & Song, S. K. (2016). Distinct immune tones are established by Lactococcus lactis BFE920 and Lactobacillus plantarum FGL0001 in the gut of olive flounder (Paralichthys olivaceus). Fish & Shellfish Immunology, 55, 434-43. https://doi.org/10.1016/j.fsi.2016.06.022
Beck BR, et al. Distinct Immune Tones Are Established By Lactococcus Lactis BFE920 and Lactobacillus Plantarum FGL0001 in the Gut of Olive Flounder (Paralichthys Olivaceus). Fish Shellfish Immunol. 2016;55:434-43. PubMed PMID: 27320869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distinct immune tones are established by Lactococcus lactis BFE920 and Lactobacillus plantarum FGL0001 in the gut of olive flounder (Paralichthys olivaceus). AU - Beck,Bo Ram, AU - Song,Joon Hyun, AU - Park,Byung Sun, AU - Kim,Daniel, AU - Kwak,Jin-Hwan, AU - Do,Hyung Ki, AU - Kim,Ah-Ram, AU - Kim,Woo-Jin, AU - Song,Seong Kyu, Y1 - 2016/06/15/ PY - 2016/04/26/received PY - 2016/06/12/revised PY - 2016/06/14/accepted PY - 2016/6/21/entrez PY - 2016/6/21/pubmed PY - 2017/3/3/medline KW - Immune tone KW - Lactobacillus plantarum FGL0001 KW - Lactococcus lactis BFE920 KW - Olive flounder KW - Probiotics SP - 434 EP - 43 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 55 N2 - The immune tone is defined as an immunological state during which the readiness for immune response is potentiated. The establishment of immune tone in the gut of olive flounder (Paralichthys olivaceus) was investigated by feeding Lactococcus lactis BFE920 (LL) or Lactobacillus plantarum FGL0001 (LP). LL-fed flounder showed significantly increased levels of regulatory genes (FOXP3, IL-10, and TGF-β1), CD18, and CD83 in the gut. In contrast, LP feeding drastically increased proinflammatory genes (T-bet, IL-1β, and IFN-γ) and CD18. This indicates that LL and LP establish different types of local immune tones in the gut through differential activation of innate immune cells: LL activates both macrophages and dendritic cells while LP activates macrophages only. Both of the immune tones required at least a total of 6 probiotic feeds during 72 h for a stable establishment. Once established, the type of immune tone remained steady even up to 30 days (a total of 60 feeds) probiotics feeding. The LL-induced regulatory immune tone enhanced the level of occludin, a tight junction molecule, significantly more than that observed with the proinflammatory immune tone established by LP feeding. Consequently, LL-fed fish showed considerably lower gut permeability than that of the LP-fed group. Furthermore, when orally challenged by Edwardsiella tarda, LL-fed flounder survived at a significantly higher rate than LP-fed fish. The data clearly demonstrate that individual probiotics establish distinct types of immune tone in the fish gut, which in turn influences the immunological status as well as the physiology of the gut. Selection of proper probiotics may be essential for optimal effects in aquaculture farming. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/27320869/Distinct_immune_tones_are_established_by_Lactococcus_lactis_BFE920_and_Lactobacillus_plantarum_FGL0001_in_the_gut_of_olive_flounder__Paralichthys_olivaceus__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(16)30382-5 DB - PRIME DP - Unbound Medicine ER -