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Ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for the structural analysis of N-unsubstituted heparin/heparan sulfate.
J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Aug 15; 1028:71-76.JC

Abstract

The rare N-unsubstituted glucosamine (GlcNH3(+)) residues in heparan sulfate (HS) have important biological and pathophysiological roles. In this study, a high-resolution method for the separation and analysis of N-unsubstituted disaccharides of heparin/HS is described. Four N-unsubstituted disaccharides, together with eight N-substituted species, can be well-separated by ion-pair reverse-phase ultra-performance liquid chromatography. Each disaccharide can then be detected and its relative abundance quantified using electrospray ionization mass spectrometry in the negative mode. Because of its high sensitivity, without interference from proteins and other sample impurities, this method is particularly useful in the analysis of low content GlcNH3(+) residues in small amounts of biological materials, eg. sera, tissue and cell culture-derived samples. This would lead to a better understanding of the biological origin of GlcNH3(+) residues and their increasingly important function in human health and disease.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Institute of Glycobiochemistry, National Engineering Research Centre of Chemical Fertilizer Catalyst, Fu Zhou University, Fu Zhou 350002, PR China.

Clinical Laboratory, Fu Zhou First Hospital, Fu Zhou, PR China.

Institute of Glycobiochemistry, National Engineering Research Centre of Chemical Fertilizer Catalyst, Fu Zhou University, Fu Zhou 350002, PR China.

Institute of Glycobiochemistry, National Engineering Research Centre of Chemical Fertilizer Catalyst, Fu Zhou University, Fu Zhou 350002, PR China.

Institute of Glycobiochemistry, National Engineering Research Centre of Chemical Fertilizer Catalyst, Fu Zhou University, Fu Zhou 350002, PR China.

Institute of Glycobiochemistry, National Engineering Research Centre of Chemical Fertilizer Catalyst, Fu Zhou University, Fu Zhou 350002, PR China.

Institute of Glycobiochemistry, National Engineering Research Centre of Chemical Fertilizer Catalyst, Fu Zhou University, Fu Zhou 350002, PR China. Electronic address: zheng0wei@hotmail.com.

MeSH

AnimalsAnticoagulantsCattleChromatography, High Pressure LiquidDisaccharidesHeparinHeparitin SulfateHumansSpectrometry, Mass, Electrospray IonizationSwine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27322632

Citation

Du, Jia Yan, et al. "Ion-pairing Liquid Chromatography With On-line Electrospray Ion Trap Mass Spectrometry for the Structural Analysis of N-unsubstituted Heparin/heparan Sulfate." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 1028, 2016, pp. 71-76.

Du JY, Chen LR, Liu S, et al. Ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for the structural analysis of N-unsubstituted heparin/heparan sulfate. J Chromatogr B Analyt Technol Biomed Life Sci. 2016;1028:71-76.

Du, J. Y., Chen, L. R., Liu, S., Lin, J. H., Liang, Q. T., Lyon, M., & Wei, Z. (2016). Ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for the structural analysis of N-unsubstituted heparin/heparan sulfate. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1028, 71-76. https://doi.org/10.1016/j.jchromb.2016.06.006

Du JY, et al. Ion-pairing Liquid Chromatography With On-line Electrospray Ion Trap Mass Spectrometry for the Structural Analysis of N-unsubstituted Heparin/heparan Sulfate. J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Aug 15;1028:71-76. PubMed PMID: 27322632.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for the structural analysis of N-unsubstituted heparin/heparan sulfate. AU - Du,Jia Yan, AU - Chen,Lan Rong, AU - Liu,Su, AU - Lin,Jiang Hui, AU - Liang,Qun Tao, AU - Lyon,Malcolm, AU - Wei,Zheng, Y1 - 2016/06/06/ PY - 2016/04/06/received PY - 2016/06/02/revised PY - 2016/06/03/accepted PY - 2016/6/21/entrez PY - 2016/6/21/pubmed PY - 2017/2/18/medline KW - Heparan sulfate KW - Heparin KW - LC–MS-ITTOF KW - N-unsubstituted disaccharide SP - 71 EP - 76 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 1028 N2 - The rare N-unsubstituted glucosamine (GlcNH3(+)) residues in heparan sulfate (HS) have important biological and pathophysiological roles. In this study, a high-resolution method for the separation and analysis of N-unsubstituted disaccharides of heparin/HS is described. Four N-unsubstituted disaccharides, together with eight N-substituted species, can be well-separated by ion-pair reverse-phase ultra-performance liquid chromatography. Each disaccharide can then be detected and its relative abundance quantified using electrospray ionization mass spectrometry in the negative mode. Because of its high sensitivity, without interference from proteins and other sample impurities, this method is particularly useful in the analysis of low content GlcNH3(+) residues in small amounts of biological materials, eg. sera, tissue and cell culture-derived samples. This would lead to a better understanding of the biological origin of GlcNH3(+) residues and their increasingly important function in human health and disease. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/27322632/Ion_pairing_liquid_chromatography_with_on_line_electrospray_ion_trap_mass_spectrometry_for_the_structural_analysis_of_N_unsubstituted_heparin/heparan_sulfate_ DB - PRIME DP - Unbound Medicine ER -