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Microarray analysis of microRNA deregulation and angiogenesis-related proteins in endometriosis.
Genet Mol Res. 2016 Jun 03; 15(2)GM

Abstract

We examined the aberrant microRNA (miRNA) expression profile responsible for the changes in angiogenesis observed in endometriotic lesions. This study revealed characteristic miRNA expression profiles associated with endometriosis in endometrial tissue and endometriotic lesions from the same patient, and their correlation with the most important angiogenic and fibrinolytic factors. miRNA expression was quantified using a microRNA array and reverse-transcription microRNA polymerase chain reaction. Levels of vascular endothelial growth factor A (VEGFA), epidermal growth factor receptor 2 (EGFR2), phosphatase and tensin homolog (PTEN), and C-X-C chemokine receptor type 4 (CXCR4) were quantified using enzyme-linked immunosorbent assay. The endometrial tissue showed significantly lower levels of miR-200b, miR-15a-5p, miR-19b-1-5p, miR-146a-5p, and miR-200c, and higher levels of miR-16-5p, miR-106b-5p, and miR-145-5p. VEGFA was significantly upregulated, whereas EGFR2, PTEN, and CXCR4 were markedly downregulated, in the endometriotic tissues compared to that in the normal endometrial tissues. In conclusion, differences in the miRNA levels could modulate the expression of VEGFA, EGFR2, PTEN, and CXCR4, and may play an important role in the pathogenesis of endometriosis. The higher angiogenic and proteolytic activities observed in the eutopic endometrium might facilitate the implantation of endometrial cells at ectopic sites.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin Province, China.Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin Province, China.Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin Province, China.Department of Anesthesia, The Second Hospital of Jilin University, Changchun, Jilin Province, China.Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin Province, China.Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin Province, China.Department of Pathology, The First Hospital of Jilin University, Changchun, Jilin Province, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27323121

Citation

Yang, R Q., et al. "Microarray Analysis of microRNA Deregulation and Angiogenesis-related Proteins in Endometriosis." Genetics and Molecular Research : GMR, vol. 15, no. 2, 2016.
Yang RQ, Teng H, Xu XH, et al. Microarray analysis of microRNA deregulation and angiogenesis-related proteins in endometriosis. Genet Mol Res. 2016;15(2).
Yang, R. Q., Teng, H., Xu, X. H., Liu, S. Y., Wang, Y. H., Guo, F. J., & Liu, X. J. (2016). Microarray analysis of microRNA deregulation and angiogenesis-related proteins in endometriosis. Genetics and Molecular Research : GMR, 15(2). https://doi.org/10.4238/gmr.15027826
Yang RQ, et al. Microarray Analysis of microRNA Deregulation and Angiogenesis-related Proteins in Endometriosis. Genet Mol Res. 2016 Jun 3;15(2) PubMed PMID: 27323121.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microarray analysis of microRNA deregulation and angiogenesis-related proteins in endometriosis. AU - Yang,R Q, AU - Teng,H, AU - Xu,X H, AU - Liu,S Y, AU - Wang,Y H, AU - Guo,F J, AU - Liu,X J, Y1 - 2016/06/03/ PY - 2016/6/21/entrez PY - 2016/6/21/pubmed PY - 2017/2/12/medline JF - Genetics and molecular research : GMR JO - Genet. Mol. Res. VL - 15 IS - 2 N2 - We examined the aberrant microRNA (miRNA) expression profile responsible for the changes in angiogenesis observed in endometriotic lesions. This study revealed characteristic miRNA expression profiles associated with endometriosis in endometrial tissue and endometriotic lesions from the same patient, and their correlation with the most important angiogenic and fibrinolytic factors. miRNA expression was quantified using a microRNA array and reverse-transcription microRNA polymerase chain reaction. Levels of vascular endothelial growth factor A (VEGFA), epidermal growth factor receptor 2 (EGFR2), phosphatase and tensin homolog (PTEN), and C-X-C chemokine receptor type 4 (CXCR4) were quantified using enzyme-linked immunosorbent assay. The endometrial tissue showed significantly lower levels of miR-200b, miR-15a-5p, miR-19b-1-5p, miR-146a-5p, and miR-200c, and higher levels of miR-16-5p, miR-106b-5p, and miR-145-5p. VEGFA was significantly upregulated, whereas EGFR2, PTEN, and CXCR4 were markedly downregulated, in the endometriotic tissues compared to that in the normal endometrial tissues. In conclusion, differences in the miRNA levels could modulate the expression of VEGFA, EGFR2, PTEN, and CXCR4, and may play an important role in the pathogenesis of endometriosis. The higher angiogenic and proteolytic activities observed in the eutopic endometrium might facilitate the implantation of endometrial cells at ectopic sites. SN - 1676-5680 UR - https://www.unboundmedicine.com/medline/citation/27323121/Microarray_analysis_of_microRNA_deregulation_and_angiogenesis_related_proteins_in_endometriosis_ L2 - http://www.geneticsmr.com/articles/6510 DB - PRIME DP - Unbound Medicine ER -