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[Effect of Electroacupuncture Intervention on Behavior Changes and Levels of Hippocampal Transforming Growth Factor beta 3 and Basic Fibroblast Growth Factor Proteins in Depression Rats].
Zhen Ci Yan Jiu. 2016 Apr; 41(2):138-43.ZC

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) on behavior changes and the adundance levels of transforming growth factor beta 3 (TGF-beta 3) and basic fibroblast growth factor (bFGF) proteins in the hippocampus of rats with chronic unpredictable mild stress (CUMS)-induced depression, so as to explore its mechanisms underlying improvement of depression.

METHODS

Forty Sprague-Dawley rats were randomly divided into the following groups: control, model, EA, and medication (Fluoxetine), n = 10 in each group. The depression model was established by CUMS combined with solitary raising for 28 days. EA (2 Hz, 0.6 mA) was applied to "Baihui" (GV 20) and "Yintang" (GV 29) for 20 mm, once daily before CUMS every day. The rats of the medication group were given with Fluoxetine (10 mg/kg, 5 mL/kg) before CUMS every day. The behavioral changes (crossing and rearing locomotion) were detected by using open field tests. The expression levels of TGF-beta 3 and bFGF proteins of the bilateral hippocampus tissues were detected using biotin label-based antibody protein chips. Results Compared to the control group, the crossed grid-square numbers and rearing times were significantly decreased in the model group (P<0.01). Following EA and medication interventions, the CUMS induced decreases of the crossed grid-square number and rearing times were notably reversed in both EA and medication groups (P<0.01), suggesting an amelioration of depression after the intervention. The relative expression level of hippocampal TGF-beta 3 was down-regulated (fold change = 0.48, vs. the control group) and that of bFGF up-regulated (fold change= 1.36, vs the control group) in the model group. In both the EA and medication groups, the down-regulated TGF-beta 3 expression and the up-regulated bFGF protein expression were suppressed (TGF-beta 3: fold change = 1.61, 1.6 and bFGF: fold change = 0.61, 0.45, vs. the model group respectively).

CONCLUSION

EA can improve the depression-like state in depression rats which may be associated with its effect in up-regulating hippocampal TGF-beta 3 protein level and down-regulating bFGF protein expression via promoting neurogenesis.

Authors

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Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

27323441

Citation

Xu, Ming-min, et al. "[Effect of Electroacupuncture Intervention On Behavior Changes and Levels of Hippocampal Transforming Growth Factor Beta 3 and Basic Fibroblast Growth Factor Proteins in Depression Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 41, no. 2, 2016, pp. 138-43.
Xu MM, Zhang DM, Shi RX, et al. [Effect of Electroacupuncture Intervention on Behavior Changes and Levels of Hippocampal Transforming Growth Factor beta 3 and Basic Fibroblast Growth Factor Proteins in Depression Rats]. Zhen Ci Yan Jiu. 2016;41(2):138-43.
Xu, M. M., Zhang, D. M., Shi, R. X., Zhang, X. H., Wang, Y., Yu, M., Guo, Y., Zhang, C. T., Zhao, B. C., Wu, J. H., & Wu, J. H. (2016). [Effect of Electroacupuncture Intervention on Behavior Changes and Levels of Hippocampal Transforming Growth Factor beta 3 and Basic Fibroblast Growth Factor Proteins in Depression Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 41(2), 138-43.
Xu MM, et al. [Effect of Electroacupuncture Intervention On Behavior Changes and Levels of Hippocampal Transforming Growth Factor Beta 3 and Basic Fibroblast Growth Factor Proteins in Depression Rats]. Zhen Ci Yan Jiu. 2016;41(2):138-43. PubMed PMID: 27323441.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Electroacupuncture Intervention on Behavior Changes and Levels of Hippocampal Transforming Growth Factor beta 3 and Basic Fibroblast Growth Factor Proteins in Depression Rats]. AU - Xu,Ming-min, AU - Zhang,Dan-mei, AU - Shi,Rong-xing, AU - Zhang,Xu-hui, AU - Wang,Yu, AU - Yu,Miao, AU - Guo,Yu, AU - Zhang,Chun-tao, AU - Zhao,Bing-cong, AU - Wu,Ji-hong, AU - Wu,Ji-hong, PY - 2016/6/22/entrez PY - 2016/6/22/pubmed PY - 2016/7/1/medline SP - 138 EP - 43 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 41 IS - 2 N2 - OBJECTIVE: To observe the effect of electroacupuncture (EA) on behavior changes and the adundance levels of transforming growth factor beta 3 (TGF-beta 3) and basic fibroblast growth factor (bFGF) proteins in the hippocampus of rats with chronic unpredictable mild stress (CUMS)-induced depression, so as to explore its mechanisms underlying improvement of depression. METHODS: Forty Sprague-Dawley rats were randomly divided into the following groups: control, model, EA, and medication (Fluoxetine), n = 10 in each group. The depression model was established by CUMS combined with solitary raising for 28 days. EA (2 Hz, 0.6 mA) was applied to "Baihui" (GV 20) and "Yintang" (GV 29) for 20 mm, once daily before CUMS every day. The rats of the medication group were given with Fluoxetine (10 mg/kg, 5 mL/kg) before CUMS every day. The behavioral changes (crossing and rearing locomotion) were detected by using open field tests. The expression levels of TGF-beta 3 and bFGF proteins of the bilateral hippocampus tissues were detected using biotin label-based antibody protein chips. Results Compared to the control group, the crossed grid-square numbers and rearing times were significantly decreased in the model group (P<0.01). Following EA and medication interventions, the CUMS induced decreases of the crossed grid-square number and rearing times were notably reversed in both EA and medication groups (P<0.01), suggesting an amelioration of depression after the intervention. The relative expression level of hippocampal TGF-beta 3 was down-regulated (fold change = 0.48, vs. the control group) and that of bFGF up-regulated (fold change= 1.36, vs the control group) in the model group. In both the EA and medication groups, the down-regulated TGF-beta 3 expression and the up-regulated bFGF protein expression were suppressed (TGF-beta 3: fold change = 1.61, 1.6 and bFGF: fold change = 0.61, 0.45, vs. the model group respectively). CONCLUSION: EA can improve the depression-like state in depression rats which may be associated with its effect in up-regulating hippocampal TGF-beta 3 protein level and down-regulating bFGF protein expression via promoting neurogenesis. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/27323441/[Effect_of_Electroacupuncture_Intervention_on_Behavior_Changes_and_Levels_of_Hippocampal_Transforming_Growth_Factor_beta_3_and_Basic_Fibroblast_Growth_Factor_Proteins_in_Depression_Rats]_ L2 - http://www.diseaseinfosearch.org/result/2199 DB - PRIME DP - Unbound Medicine ER -