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Evidence of Resistance to Cry34/35Ab1 Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae): Root Injury in the Field and Larval Survival in Plant-Based Bioassays.
J Econ Entomol. 2016 Aug; 109(4):1872-80.JE

Abstract

Western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a serious pest of corn in the United States, and recent management of western corn rootworm has included planting of Bt corn. Beginning in 2009, western corn rootworm populations with resistance to Cry3Bb1 corn and mCry3A corn were found in Iowa and elsewhere. To date, western corn rootworm populations have remained susceptible to corn producing Bt toxin Cry34/35Ab1. In this study, we used single-plant bioassays to test field populations of western corn rootworm for resistance to Cry34/35Ab1 corn, Cry3Bb1 corn, and mCry3A corn. Bioassays included nine rootworm populations collected from fields where severe injury to Bt corn had been observed and six control populations that had never been exposed to Bt corn. We found incomplete resistance to Cry34/35Ab1 corn among field populations collected from fields where severe injury to corn producing Cry34/35Ab1, either singly or as a pyramid, had been observed. Additionally, resistance to Cry3Bb1 corn and mCry3A corn was found among the majority of populations tested. These first cases of resistance to Cry34/35Ab1 corn, and the presence of resistance to multiple Bt toxins by western corn rootworm, highlight the potential vulnerability of Bt corn to the evolution of resistance by western corn rootworm. The use of more diversified management practices, in addition to insect resistance management, likely will be essential to sustain the viability of Bt corn for management of western corn rootworm.

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Authors+Show Affiliations

Gassmann AJ
Department of Entomology, Iowa State University, Ames, IA 50011 (aaronjg@iastate.edu; shrestrb@iastate.edu; dunbar@iastate.edu; eclifton@iastate.edu; apaolino@iastate.edu; masloski@iastate.edu; jdoudna@iastate.edu; cstclair@iastate.edu), Corresponding author, e-mail: aaronjg@iastate.edu.
Shrestha RB
Department of Entomology, Iowa State University, Ames, IA 50011 (aaronjg@iastate.edu; shrestrb@iastate.edu; dunbar@iastate.edu; eclifton@iastate.edu; apaolino@iastate.edu; masloski@iastate.edu; jdoudna@iastate.edu; cstclair@iastate.edu).
Jakka SR
Department of Entomology, Iowa State University, Ames, IA 50011 (aaronjg@iastate.edu; shrestrb@iastate.edu; dunbar@iastate.edu; eclifton@iastate.edu; apaolino@iastate.edu; masloski@iastate.edu; jdoudna@iastate.edu; cstclair@iastate.edu), Current address: Valent U.S.A. Company, 3800 Old Leland Rd., Leland, MS 38756 (sjakk@valent.com).
Dunbar MW
Department of Entomology, Iowa State University, Ames, IA 50011 (aaronjg@iastate.edu; shrestrb@iastate.edu; dunbar@iastate.edu; eclifton@iastate.edu; apaolino@iastate.edu; masloski@iastate.edu; jdoudna@iastate.edu; cstclair@iastate.edu).
Clifton EH
Department of Entomology, Iowa State University, Ames, IA 50011 (aaronjg@iastate.edu; shrestrb@iastate.edu; dunbar@iastate.edu; eclifton@iastate.edu; apaolino@iastate.edu; masloski@iastate.edu; jdoudna@iastate.edu; cstclair@iastate.edu).
Paolino AR
Department of Entomology, Iowa State University, Ames, IA 50011 (aaronjg@iastate.edu; shrestrb@iastate.edu; dunbar@iastate.edu; eclifton@iastate.edu; apaolino@iastate.edu; masloski@iastate.edu; jdoudna@iastate.edu; cstclair@iastate.edu).
Ingber DA
Department of Entomology, Iowa State University, Ames, IA 50011 (aaronjg@iastate.edu; shrestrb@iastate.edu; dunbar@iastate.edu; eclifton@iastate.edu; apaolino@iastate.edu; masloski@iastate.edu; jdoudna@iastate.edu; cstclair@iastate.edu), Current address: Department of Entomology and Wildlife Ecology, University of Delaware, Newark, DE 19716 (davidngbr@gmail.com), and.
French BW
USDA ARS North Central Agricultural Research Laboratory, Brookings, SD (wade.french@ars.usda.gov).
Masloski KE
Department of Entomology, Iowa State University, Ames, IA 50011 (aaronjg@iastate.edu; shrestrb@iastate.edu; dunbar@iastate.edu; eclifton@iastate.edu; apaolino@iastate.edu; masloski@iastate.edu; jdoudna@iastate.edu; cstclair@iastate.edu).
Dounda JW
Department of Entomology, Iowa State University, Ames, IA 50011 (aaronjg@iastate.edu; shrestrb@iastate.edu; dunbar@iastate.edu; eclifton@iastate.edu; apaolino@iastate.edu; masloski@iastate.edu; jdoudna@iastate.edu; cstclair@iastate.edu).
St Clair CR
Department of Entomology, Iowa State University, Ames, IA 50011 (aaronjg@iastate.edu; shrestrb@iastate.edu; dunbar@iastate.edu; eclifton@iastate.edu; apaolino@iastate.edu; masloski@iastate.edu; jdoudna@iastate.edu; cstclair@iastate.edu).

MeSH

AnimalsBacillus thuringiensisBacillus thuringiensis ToxinsBacterial ProteinsColeopteraEndotoxinsHemolysin ProteinsInsecticide ResistanceLarvaPlant RootsPlants, Genetically ModifiedZea mays

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27329619

Citation

Gassmann, Aaron J., et al. "Evidence of Resistance to Cry34/35Ab1 Corn By Western Corn Rootworm (Coleoptera: Chrysomelidae): Root Injury in the Field and Larval Survival in Plant-Based Bioassays." Journal of Economic Entomology, vol. 109, no. 4, 2016, pp. 1872-80.
Gassmann AJ, Shrestha RB, Jakka SR, et al. Evidence of Resistance to Cry34/35Ab1 Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae): Root Injury in the Field and Larval Survival in Plant-Based Bioassays. J Econ Entomol. 2016;109(4):1872-80.
Gassmann, A. J., Shrestha, R. B., Jakka, S. R., Dunbar, M. W., Clifton, E. H., Paolino, A. R., Ingber, D. A., French, B. W., Masloski, K. E., Dounda, J. W., & St Clair, C. R. (2016). Evidence of Resistance to Cry34/35Ab1 Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae): Root Injury in the Field and Larval Survival in Plant-Based Bioassays. Journal of Economic Entomology, 109(4), 1872-80. https://doi.org/10.1093/jee/tow110
Gassmann AJ, et al. Evidence of Resistance to Cry34/35Ab1 Corn By Western Corn Rootworm (Coleoptera: Chrysomelidae): Root Injury in the Field and Larval Survival in Plant-Based Bioassays. J Econ Entomol. 2016;109(4):1872-80. PubMed PMID: 27329619.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence of Resistance to Cry34/35Ab1 Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae): Root Injury in the Field and Larval Survival in Plant-Based Bioassays. AU - Gassmann,Aaron J, AU - Shrestha,Ram B, AU - Jakka,Siva R K, AU - Dunbar,Mike W, AU - Clifton,Eric H, AU - Paolino,Aubrey R, AU - Ingber,David A, AU - French,B Wade, AU - Masloski,Kenneth E, AU - Dounda,John W, AU - St Clair,Coy R, Y1 - 2016/06/21/ PY - 2016/01/12/received PY - 2016/05/03/accepted PY - 2016/6/23/entrez PY - 2016/6/23/pubmed PY - 2017/11/18/medline KW - Diabrotica virgifera virgifera KW - bioassay KW - insect resistance management KW - integrated pest management KW - refuge strategy SP - 1872 EP - 80 JF - Journal of economic entomology JO - J Econ Entomol VL - 109 IS - 4 N2 - Western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a serious pest of corn in the United States, and recent management of western corn rootworm has included planting of Bt corn. Beginning in 2009, western corn rootworm populations with resistance to Cry3Bb1 corn and mCry3A corn were found in Iowa and elsewhere. To date, western corn rootworm populations have remained susceptible to corn producing Bt toxin Cry34/35Ab1. In this study, we used single-plant bioassays to test field populations of western corn rootworm for resistance to Cry34/35Ab1 corn, Cry3Bb1 corn, and mCry3A corn. Bioassays included nine rootworm populations collected from fields where severe injury to Bt corn had been observed and six control populations that had never been exposed to Bt corn. We found incomplete resistance to Cry34/35Ab1 corn among field populations collected from fields where severe injury to corn producing Cry34/35Ab1, either singly or as a pyramid, had been observed. Additionally, resistance to Cry3Bb1 corn and mCry3A corn was found among the majority of populations tested. These first cases of resistance to Cry34/35Ab1 corn, and the presence of resistance to multiple Bt toxins by western corn rootworm, highlight the potential vulnerability of Bt corn to the evolution of resistance by western corn rootworm. The use of more diversified management practices, in addition to insect resistance management, likely will be essential to sustain the viability of Bt corn for management of western corn rootworm. SN - 1938-291X UR - https://www.unboundmedicine.com/medline/citation/27329619/Evidence_of_Resistance_to_Cry34/35Ab1_Corn_by_Western_Corn_Rootworm__Coleoptera:_Chrysomelidae_:_Root_Injury_in_the_Field_and_Larval_Survival_in_Plant_Based_Bioassays_ DB - PRIME DP - Unbound Medicine ER -