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Validated RP-HPLC method for simultaneous determination and quantification of chlorpheniramine maleate, paracetamol and caffeine in tablet formulation.
Springerplus. 2016; 5:625.S

Abstract

Chlorpheniramine maleate-paracetamol-caffeine tablet formulation is one of the common over-the-counter drugs used for the treatment of cold and cough. A reversed-phase high-performance liquid-chromatography method has been successfully developed for the simultaneous determination of chlorpheniramine maleate, paracetamol and caffeine in a drug formulation. The RP-HPLC method employed a Phenomenex C18 reversed phase column (Luna 5µ, 250 × 4.6 mm) with an isocratic mixture of methanol and 0.05 M dibasic phosphate buffer pH 4.0 in the ratio of (30:70; v/v) as the mobile phase. The column temperature was kept at 30 °C. The flow rate was 1.0 mL/min and detection was by means of a UV detector at wavelength of 215 nm. All the active components were successfully eluted with mean retention times of 2.4, 4.2, 7.2 min for chlorpheniramine maleate, paracetamol and caffeine respectively. The method was found to be linear (R(2) > 0.99), precise (RSD < 2.0 %), accurate (recoveries 97.9-102.8 %), specific, simple, sensitive, rapid and robust. The validated method can be used in routine quality control analysis of fixed dose combination tablets containing chlorpheniramine maleate, paracetamol and caffeine without any interference by excipients.

Authors+Show Affiliations

Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.Department of Chemistry, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana ; Department of Pharmaceutical Science, Faculty of Medicine and Health Science, Kumasi Polytechnic, Kumasi, Ghana.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27330891

Citation

Acheampong, Akwasi, et al. "Validated RP-HPLC Method for Simultaneous Determination and Quantification of Chlorpheniramine Maleate, Paracetamol and Caffeine in Tablet Formulation." SpringerPlus, vol. 5, 2016, p. 625.
Acheampong A, Gyasi WO, Darko G, et al. Validated RP-HPLC method for simultaneous determination and quantification of chlorpheniramine maleate, paracetamol and caffeine in tablet formulation. Springerplus. 2016;5:625.
Acheampong, A., Gyasi, W. O., Darko, G., Apau, J., & Addai-Arhin, S. (2016). Validated RP-HPLC method for simultaneous determination and quantification of chlorpheniramine maleate, paracetamol and caffeine in tablet formulation. SpringerPlus, 5, 625. https://doi.org/10.1186/s40064-016-2241-2
Acheampong A, et al. Validated RP-HPLC Method for Simultaneous Determination and Quantification of Chlorpheniramine Maleate, Paracetamol and Caffeine in Tablet Formulation. Springerplus. 2016;5:625. PubMed PMID: 27330891.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validated RP-HPLC method for simultaneous determination and quantification of chlorpheniramine maleate, paracetamol and caffeine in tablet formulation. AU - Acheampong,Akwasi, AU - Gyasi,Wilfred Owusu, AU - Darko,Godfred, AU - Apau,Joseph, AU - Addai-Arhin,Sylvester, Y1 - 2016/05/14/ PY - 2015/12/26/received PY - 2016/04/27/accepted PY - 2016/6/23/entrez PY - 2016/6/23/pubmed PY - 2016/6/23/medline KW - Caffeine KW - Chlorpheniramine maleate KW - Cyanocobalamin KW - Multi-component formulation KW - Paracetamol KW - RP-HPLC KW - Tablet formulations SP - 625 EP - 625 JF - SpringerPlus JO - Springerplus VL - 5 N2 - Chlorpheniramine maleate-paracetamol-caffeine tablet formulation is one of the common over-the-counter drugs used for the treatment of cold and cough. A reversed-phase high-performance liquid-chromatography method has been successfully developed for the simultaneous determination of chlorpheniramine maleate, paracetamol and caffeine in a drug formulation. The RP-HPLC method employed a Phenomenex C18 reversed phase column (Luna 5µ, 250 × 4.6 mm) with an isocratic mixture of methanol and 0.05 M dibasic phosphate buffer pH 4.0 in the ratio of (30:70; v/v) as the mobile phase. The column temperature was kept at 30 °C. The flow rate was 1.0 mL/min and detection was by means of a UV detector at wavelength of 215 nm. All the active components were successfully eluted with mean retention times of 2.4, 4.2, 7.2 min for chlorpheniramine maleate, paracetamol and caffeine respectively. The method was found to be linear (R(2) > 0.99), precise (RSD < 2.0 %), accurate (recoveries 97.9-102.8 %), specific, simple, sensitive, rapid and robust. The validated method can be used in routine quality control analysis of fixed dose combination tablets containing chlorpheniramine maleate, paracetamol and caffeine without any interference by excipients. SN - 2193-1801 UR - https://www.unboundmedicine.com/medline/citation/27330891/Validated_RP_HPLC_method_for_simultaneous_determination_and_quantification_of_chlorpheniramine_maleate_paracetamol_and_caffeine_in_tablet_formulation_ L2 - https://dx.doi.org/10.1186/s40064-016-2241-2 DB - PRIME DP - Unbound Medicine ER -
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