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Overexpression of OsMYC2 Results in the Up-Regulation of Early JA-Rresponsive Genes and Bacterial Blight Resistance in Rice.
Plant Cell Physiol 2016; 57(9):1814-27PC

Abstract

JASMONATE ZIM-domain (JAZ) proteins act as transcriptional repressors of jasmonic acid (JA) responses and play a crucial role in the regulation of host immunity in plants. Here, we report that OsMYC2, a JAZ-interacting transcription factor in rice (Oryza sativa L.), plays an important role in the resistance response against rice bacterial blight, which is one of the most serious diseases in rice, caused by Xanthomonas oryzae pv. oryzae (Xoo). The results showed that OsMYC2 interacted with some OsJAZ proteins in a JAZ-interacting domain (JID)-dependent manner. The up-regulation of OsMYC2 in response to JA was regulated by OsJAZ8. Transgenic rice plants overexpressing OsMYC2 exhibited a JA-hypersensitive phenotype and were more resistant to Xoo. A large-scale microarray analysis revealed that OsMYC2 up-regulated OsJAZ10 as well as many other defense-related genes. OsMYC2 selectively bound to the G-box-like motif of the OsJAZ10 promoter in vivo and regulated the expression of early JA-responsive genes, but not of late JA-responsive genes. The nuclear localization of OsMYC2 depended on a nuclear localization signal within JID. Overall, we conclude that OsMYC2 acts as a positive regulator of early JA signals in the JA-induced resistance against Xoo in rice.

Authors+Show Affiliations

Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795 Japan.Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795 Japan.Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795 Japan.Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795 Japan.Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795 Japan.Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795 Japan gomiken@ag.kagawa-u.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27335352

Citation

Uji, Yuya, et al. "Overexpression of OsMYC2 Results in the Up-Regulation of Early JA-Rresponsive Genes and Bacterial Blight Resistance in Rice." Plant & Cell Physiology, vol. 57, no. 9, 2016, pp. 1814-27.
Uji Y, Taniguchi S, Tamaoki D, et al. Overexpression of OsMYC2 Results in the Up-Regulation of Early JA-Rresponsive Genes and Bacterial Blight Resistance in Rice. Plant Cell Physiol. 2016;57(9):1814-27.
Uji, Y., Taniguchi, S., Tamaoki, D., Shishido, H., Akimitsu, K., & Gomi, K. (2016). Overexpression of OsMYC2 Results in the Up-Regulation of Early JA-Rresponsive Genes and Bacterial Blight Resistance in Rice. Plant & Cell Physiology, 57(9), pp. 1814-27. doi:10.1093/pcp/pcw101.
Uji Y, et al. Overexpression of OsMYC2 Results in the Up-Regulation of Early JA-Rresponsive Genes and Bacterial Blight Resistance in Rice. Plant Cell Physiol. 2016;57(9):1814-27. PubMed PMID: 27335352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpression of OsMYC2 Results in the Up-Regulation of Early JA-Rresponsive Genes and Bacterial Blight Resistance in Rice. AU - Uji,Yuya, AU - Taniguchi,Shiduku, AU - Tamaoki,Daisuke, AU - Shishido,Hodaka, AU - Akimitsu,Kazuya, AU - Gomi,Kenji, Y1 - 2016/05/19/ PY - 2016/02/23/received PY - 2016/05/12/accepted PY - 2016/6/24/entrez PY - 2016/6/24/pubmed PY - 2017/3/14/medline KW - JAZ protein KW - Jasmonic acid KW - Oryza sativa KW - Transcription factor KW - Xanthomonas oryzae SP - 1814 EP - 27 JF - Plant & cell physiology JO - Plant Cell Physiol. VL - 57 IS - 9 N2 - JASMONATE ZIM-domain (JAZ) proteins act as transcriptional repressors of jasmonic acid (JA) responses and play a crucial role in the regulation of host immunity in plants. Here, we report that OsMYC2, a JAZ-interacting transcription factor in rice (Oryza sativa L.), plays an important role in the resistance response against rice bacterial blight, which is one of the most serious diseases in rice, caused by Xanthomonas oryzae pv. oryzae (Xoo). The results showed that OsMYC2 interacted with some OsJAZ proteins in a JAZ-interacting domain (JID)-dependent manner. The up-regulation of OsMYC2 in response to JA was regulated by OsJAZ8. Transgenic rice plants overexpressing OsMYC2 exhibited a JA-hypersensitive phenotype and were more resistant to Xoo. A large-scale microarray analysis revealed that OsMYC2 up-regulated OsJAZ10 as well as many other defense-related genes. OsMYC2 selectively bound to the G-box-like motif of the OsJAZ10 promoter in vivo and regulated the expression of early JA-responsive genes, but not of late JA-responsive genes. The nuclear localization of OsMYC2 depended on a nuclear localization signal within JID. Overall, we conclude that OsMYC2 acts as a positive regulator of early JA signals in the JA-induced resistance against Xoo in rice. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/27335352/Overexpression_of_OsMYC2_Results_in_the_Up_Regulation_of_Early_JA_Rresponsive_Genes_and_Bacterial_Blight_Resistance_in_Rice_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcw101 DB - PRIME DP - Unbound Medicine ER -