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Overexpression of lncRNA UCA1 promotes osteosarcoma progression and correlates with poor prognosis.
J Bone Oncol 2016; 5(2):80-5JB

Abstract

Long non-coding RNAs (lncRNAs) have been proved to play important roles in the tumorigenesis and development of several human malignancies. Our study aims to investigate the expression and function of lncRNA-UCA1 in osteosarcoma. lncRNA-UCA1 expression was detected in osteosarcoma tissues and cell lines by using qRT-PCR. Association between lncRNA-UCA1 levels and clinicopathological factors and patient's prognosis was analyzed. The roles of lncRNA-UCA1 in regulating osteosarcoma cell proliferation, apoptosis, migration, and invasion were evaluated in vitro. We found that lncRNA-UCA1 expression was upregulated in osteosarcoma tissues and cell lines. High lncRNA-UCA1 expression was significantly correlated with large tumor size, high tumor grade, positive distant metastasis, and advanced clinical stage. Multivariate regression analysis identified lncRNA-UCA1 overexpression as an independent unfavorable prognostic factor. lncRNA-UCA1 knockdown inhibited osteosarcoma cell proliferation, promoted cell apoptosis, and suppressed cell invasion and migration, whereas lncRNA-UCA1 overexpression showed opposite effects. These findings suggested that lncRNA-UCA1 may contribute to osteosarcoma initiation and progression, and would be not only a novel prognostic marker but also a potential therapeutic target for this disease.

Authors+Show Affiliations

Department of spine surgery, Hanzhong Municipal Central Hospital, Hanzhong 723000, Shaanxi Province, China.Department of bone and joint trauma, Hanzhong Municipal Central Hospital, Hanzhong 723000, Shaanxi Province, China.Department of bone and joint trauma, Hanzhong Municipal Central Hospital, Hanzhong 723000, Shaanxi Province, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27335776

Citation

Li, Wei, et al. "Overexpression of lncRNA UCA1 Promotes Osteosarcoma Progression and Correlates With Poor Prognosis." Journal of Bone Oncology, vol. 5, no. 2, 2016, pp. 80-5.
Li W, Xie P, Ruan WH. Overexpression of lncRNA UCA1 promotes osteosarcoma progression and correlates with poor prognosis. J Bone Oncol. 2016;5(2):80-5.
Li, W., Xie, P., & Ruan, W. H. (2016). Overexpression of lncRNA UCA1 promotes osteosarcoma progression and correlates with poor prognosis. Journal of Bone Oncology, 5(2), pp. 80-5. doi:10.1016/j.jbo.2016.05.003.
Li W, Xie P, Ruan WH. Overexpression of lncRNA UCA1 Promotes Osteosarcoma Progression and Correlates With Poor Prognosis. J Bone Oncol. 2016;5(2):80-5. PubMed PMID: 27335776.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpression of lncRNA UCA1 promotes osteosarcoma progression and correlates with poor prognosis. AU - Li,Wei, AU - Xie,Peng, AU - Ruan,Wen-Hui, Y1 - 2016/05/10/ PY - 2016/04/24/received PY - 2016/05/06/accepted PY - 2016/6/24/entrez PY - 2016/6/24/pubmed PY - 2016/6/24/medline KW - DMEM, Dulbecco's modified Eagle's medium KW - DMSO, dimethyl sulfoxide KW - Invasion KW - LncRNA-UCA1 KW - MTT, 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide KW - Osteosarcoma KW - Prognosis KW - RT-PCR, reverse transcription-polymerase chain reaction KW - TNM, tumor-node-metastasis KW - UCA1, Urothelial carcinoma associated 1 KW - lncRNA, long noncoding RNA SP - 80 EP - 5 JF - Journal of bone oncology JO - J Bone Oncol VL - 5 IS - 2 N2 - Long non-coding RNAs (lncRNAs) have been proved to play important roles in the tumorigenesis and development of several human malignancies. Our study aims to investigate the expression and function of lncRNA-UCA1 in osteosarcoma. lncRNA-UCA1 expression was detected in osteosarcoma tissues and cell lines by using qRT-PCR. Association between lncRNA-UCA1 levels and clinicopathological factors and patient's prognosis was analyzed. The roles of lncRNA-UCA1 in regulating osteosarcoma cell proliferation, apoptosis, migration, and invasion were evaluated in vitro. We found that lncRNA-UCA1 expression was upregulated in osteosarcoma tissues and cell lines. High lncRNA-UCA1 expression was significantly correlated with large tumor size, high tumor grade, positive distant metastasis, and advanced clinical stage. Multivariate regression analysis identified lncRNA-UCA1 overexpression as an independent unfavorable prognostic factor. lncRNA-UCA1 knockdown inhibited osteosarcoma cell proliferation, promoted cell apoptosis, and suppressed cell invasion and migration, whereas lncRNA-UCA1 overexpression showed opposite effects. These findings suggested that lncRNA-UCA1 may contribute to osteosarcoma initiation and progression, and would be not only a novel prognostic marker but also a potential therapeutic target for this disease. SN - 2212-1366 UR - https://www.unboundmedicine.com/medline/citation/27335776/Overexpression_of_lncRNA_UCA1_promotes_osteosarcoma_progression_and_correlates_with_poor_prognosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2212-1374(16)30040-9 DB - PRIME DP - Unbound Medicine ER -