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Venomic Analysis of the Poorly Studied Desert Coral Snake, Micrurus tschudii tschudii, Supports the 3FTx/PLA₂ Dichotomy across Micrurus Venoms.
Toxins (Basel). 2016 06 07; 8(6)T

Abstract

The venom proteome of the poorly studied desert coral snake Micrurus tschudii tschudii was unveiled using a venomic approach, which identified ≥38 proteins belonging to only four snake venom protein families. The three-finger toxins (3FTxs) constitute, both in number of isoforms (~30) and total abundance (93.6% of the venom proteome), the major protein family of the desert coral snake venom. Phospholipases A₂ (PLA₂s; seven isoforms, 4.1% of the venom proteome), 1-3 Kunitz-type proteins (1.6%), and 1-2 l-amino acid oxidases (LAO, 0.7%) complete the toxin arsenal of M. t. tschudii. Our results add to the growing evidence that the occurrence of two divergent venom phenotypes, i.e., 3FTx- and PLA₂-predominant venom proteomes, may constitute a general trend across the cladogenesis of Micrurus. The occurrence of a similar pattern of venom phenotypic variability among true sea snake (Hydrophiinae) venoms suggests that the 3FTx/PLA₂ dichotomy may be widely distributed among Elapidae venoms.

Authors+Show Affiliations

Laboratorio de Venómica Estructural y Funcional, Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, Valencia 46010, Spain. libia.sanz@ibv.csic.es.Laboratorio de Venómica Estructural y Funcional, Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, Valencia 46010, Spain. dpla@ibv.csic.es.Laboratorio de Venómica Estructural y Funcional, Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, Valencia 46010, Spain. aperez@ibv.csic.es.Laboratorio de Venómica Estructural y Funcional, Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, Valencia 46010, Spain. yrodriguez@ibv.csic.es.Departamento Academico de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima 31, Perú. alfonso.zavaleta@upch.pe. Instituto Nacional de Salud, Ministerio de Salud, Lima 11, Perú. alfonso.zavaleta@upch.pe.Departamento Academico de Ciencias Celulares y Moleculares, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima 31, Perú. maria.salas@upch.pe.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San Jose 11501, Costa Rica. bruno.lomonte@ucr.ac.cr.Laboratorio de Venómica Estructural y Funcional, Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, Valencia 46010, Spain. jcalvete@ibv.csic.es.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27338473

Citation

Sanz, Libia, et al. "Venomic Analysis of the Poorly Studied Desert Coral Snake, Micrurus Tschudii Tschudii, Supports the 3FTx/PLA₂ Dichotomy Across Micrurus Venoms." Toxins, vol. 8, no. 6, 2016.
Sanz L, Pla D, Pérez A, et al. Venomic Analysis of the Poorly Studied Desert Coral Snake, Micrurus tschudii tschudii, Supports the 3FTx/PLA₂ Dichotomy across Micrurus Venoms. Toxins (Basel). 2016;8(6).
Sanz, L., Pla, D., Pérez, A., Rodríguez, Y., Zavaleta, A., Salas, M., Lomonte, B., & Calvete, J. J. (2016). Venomic Analysis of the Poorly Studied Desert Coral Snake, Micrurus tschudii tschudii, Supports the 3FTx/PLA₂ Dichotomy across Micrurus Venoms. Toxins, 8(6). https://doi.org/10.3390/toxins8060178
Sanz L, et al. Venomic Analysis of the Poorly Studied Desert Coral Snake, Micrurus Tschudii Tschudii, Supports the 3FTx/PLA₂ Dichotomy Across Micrurus Venoms. Toxins (Basel). 2016 06 7;8(6) PubMed PMID: 27338473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Venomic Analysis of the Poorly Studied Desert Coral Snake, Micrurus tschudii tschudii, Supports the 3FTx/PLA₂ Dichotomy across Micrurus Venoms. AU - Sanz,Libia, AU - Pla,Davinia, AU - Pérez,Alicia, AU - Rodríguez,Yania, AU - Zavaleta,Alfonso, AU - Salas,Maria, AU - Lomonte,Bruno, AU - Calvete,Juan J, Y1 - 2016/06/07/ PY - 2016/03/23/received PY - 2016/05/17/revised PY - 2016/06/01/accepted PY - 2016/6/25/entrez PY - 2016/6/25/pubmed PY - 2017/11/29/medline KW - Micrurus tschudii tschudii venom KW - mass spectrometry KW - snake venom phospholipase A2 KW - snake venom proteome KW - three-finger toxin KW - venomics JF - Toxins JO - Toxins (Basel) VL - 8 IS - 6 N2 - The venom proteome of the poorly studied desert coral snake Micrurus tschudii tschudii was unveiled using a venomic approach, which identified ≥38 proteins belonging to only four snake venom protein families. The three-finger toxins (3FTxs) constitute, both in number of isoforms (~30) and total abundance (93.6% of the venom proteome), the major protein family of the desert coral snake venom. Phospholipases A₂ (PLA₂s; seven isoforms, 4.1% of the venom proteome), 1-3 Kunitz-type proteins (1.6%), and 1-2 l-amino acid oxidases (LAO, 0.7%) complete the toxin arsenal of M. t. tschudii. Our results add to the growing evidence that the occurrence of two divergent venom phenotypes, i.e., 3FTx- and PLA₂-predominant venom proteomes, may constitute a general trend across the cladogenesis of Micrurus. The occurrence of a similar pattern of venom phenotypic variability among true sea snake (Hydrophiinae) venoms suggests that the 3FTx/PLA₂ dichotomy may be widely distributed among Elapidae venoms. SN - 2072-6651 UR - https://www.unboundmedicine.com/medline/citation/27338473/Venomic_Analysis_of_the_Poorly_Studied_Desert_Coral_Snake_Micrurus_tschudii_tschudii_Supports_the_3FTx/PLA₂_Dichotomy_across_Micrurus_Venoms_ L2 - https://www.mdpi.com/resolver?pii=toxins8060178 DB - PRIME DP - Unbound Medicine ER -