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Long-pulse gastric electrical stimulation protects interstitial cells of Cajal in diabetic rats via IGF-1 signaling pathway.
World J Gastroenterol. 2016 Jun 21; 22(23):5353-63.WJ

Abstract

AIM

To investigate the effects of different parameters of gastric electrical stimulation (GES) on interstitial cells of Cajal (ICCs) and changes in the insulin-like growth factor 1 (IGF-1) signal pathway in streptozotocin-induced diabetic rats.

METHODS

Male rats were randomized into control, diabetic (DM), diabetic with sham GES (DM + SGES), diabetic with GES1 (5.5 cpm, 100 ms, 4 mA) (DM + GES1), diabetic with GES2 (5.5 cpm, 300 ms, 4 mA) (DM + GES2) and diabetic with GES3 (5.5 cpm, 550 ms, 2 mA) (DM + GES3) groups. The expression levels of c-kit, M-SCF and IGF-1 receptors were evaluated in the gastric antrum using Western blot analysis. The distribution of ICCs was observed using immunolabeling for c-kit, while smooth muscle cells and IGF-1 receptors were identified using α-SMA and IGF-1R antibodies. Serum level of IGF-1 was tested using enzyme-linked immunosorbent assay.

RESULTS

Gastric emptying was delayed in the DM group but improved in all GES groups, especially in the GES2 group. The expression levels of c-kit, M-SCF and IGF-1R were decreased in the DM group but increased in all GES groups. More ICCs (c-kit(+)) and smooth muscle cells (α-SMA(+)/IGF-1R(+)) were observed in all GES groups than in the DM group. The average level of IGF-1 in the DM group was markedly decreased, but it was up-regulated in all GES groups, especially in the GES2 group.

CONCLUSION

The results suggest that long-pulse GES promotes the regeneration of ICCs. The IGF-1 signaling pathway might be involved in the mechanism underlying this process, which results in improved gastric emptying.

Authors+Show Affiliations

Hai Li, Yan Chen, Shi Liu, Xiao-Hua Hou, Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.Hai Li, Yan Chen, Shi Liu, Xiao-Hua Hou, Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.Hai Li, Yan Chen, Shi Liu, Xiao-Hua Hou, Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.Hai Li, Yan Chen, Shi Liu, Xiao-Hua Hou, Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27340351

Citation

Li, Hai, et al. "Long-pulse Gastric Electrical Stimulation Protects Interstitial Cells of Cajal in Diabetic Rats Via IGF-1 Signaling Pathway." World Journal of Gastroenterology, vol. 22, no. 23, 2016, pp. 5353-63.
Li H, Chen Y, Liu S, et al. Long-pulse gastric electrical stimulation protects interstitial cells of Cajal in diabetic rats via IGF-1 signaling pathway. World J Gastroenterol. 2016;22(23):5353-63.
Li, H., Chen, Y., Liu, S., & Hou, X. H. (2016). Long-pulse gastric electrical stimulation protects interstitial cells of Cajal in diabetic rats via IGF-1 signaling pathway. World Journal of Gastroenterology, 22(23), 5353-63. https://doi.org/10.3748/wjg.v22.i23.5353
Li H, et al. Long-pulse Gastric Electrical Stimulation Protects Interstitial Cells of Cajal in Diabetic Rats Via IGF-1 Signaling Pathway. World J Gastroenterol. 2016 Jun 21;22(23):5353-63. PubMed PMID: 27340351.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-pulse gastric electrical stimulation protects interstitial cells of Cajal in diabetic rats via IGF-1 signaling pathway. AU - Li,Hai, AU - Chen,Yan, AU - Liu,Shi, AU - Hou,Xiao-Hua, PY - 2016/03/01/received PY - 2016/04/14/revised PY - 2016/05/04/accepted PY - 2016/6/25/entrez PY - 2016/6/25/pubmed PY - 2017/4/27/medline KW - Diabetes mellitus KW - Gastric emptying KW - Gastrointestinal motility disorders KW - IGF-1 pathway KW - Interstitial cells of Cajal KW - Long-pulse gastric electrical stimulation SP - 5353 EP - 63 JF - World journal of gastroenterology JO - World J Gastroenterol VL - 22 IS - 23 N2 - AIM: To investigate the effects of different parameters of gastric electrical stimulation (GES) on interstitial cells of Cajal (ICCs) and changes in the insulin-like growth factor 1 (IGF-1) signal pathway in streptozotocin-induced diabetic rats. METHODS: Male rats were randomized into control, diabetic (DM), diabetic with sham GES (DM + SGES), diabetic with GES1 (5.5 cpm, 100 ms, 4 mA) (DM + GES1), diabetic with GES2 (5.5 cpm, 300 ms, 4 mA) (DM + GES2) and diabetic with GES3 (5.5 cpm, 550 ms, 2 mA) (DM + GES3) groups. The expression levels of c-kit, M-SCF and IGF-1 receptors were evaluated in the gastric antrum using Western blot analysis. The distribution of ICCs was observed using immunolabeling for c-kit, while smooth muscle cells and IGF-1 receptors were identified using α-SMA and IGF-1R antibodies. Serum level of IGF-1 was tested using enzyme-linked immunosorbent assay. RESULTS: Gastric emptying was delayed in the DM group but improved in all GES groups, especially in the GES2 group. The expression levels of c-kit, M-SCF and IGF-1R were decreased in the DM group but increased in all GES groups. More ICCs (c-kit(+)) and smooth muscle cells (α-SMA(+)/IGF-1R(+)) were observed in all GES groups than in the DM group. The average level of IGF-1 in the DM group was markedly decreased, but it was up-regulated in all GES groups, especially in the GES2 group. CONCLUSION: The results suggest that long-pulse GES promotes the regeneration of ICCs. The IGF-1 signaling pathway might be involved in the mechanism underlying this process, which results in improved gastric emptying. SN - 2219-2840 UR - https://www.unboundmedicine.com/medline/citation/27340351/Long_pulse_gastric_electrical_stimulation_protects_interstitial_cells_of_Cajal_in_diabetic_rats_via_IGF_1_signaling_pathway_ DB - PRIME DP - Unbound Medicine ER -