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Alphacoronavirus in urban Molossidae and Phyllostomidae bats, Brazil.
Virol J. 2016 06 24; 13:110.VJ

Abstract

BACKGROUND

Bats have been implicated as the main reservoir of coronavirus (CoV). Thus the role of these hosts on the evolution and spread of CoVs currently deserve the attention of emerging diseases surveillance programs. On the view of the interest on and importance of CoVs in bats the occurrence and molecular characterization of CoV were conducted in bats from Brazil.

FINDINGS

Three hundred five enteric contents of 29 bat species were tested using a panCoV nested RT-PCR. Nine specimens were positive and eight was suitable for RdRp gene sequencing. RdRp gene phylogeny showed that all CoVs strains from this study cluster in Alphacoronavirus genus, with one Molossidae and one Phlyllostomidae-CoV specific groups. Phylogenetic analyses of two S gene sequences showed a large diversity within the Alphacoronavirus genus.

CONCLUSIONS

This study indicated a CoV-to-host specificity and draws attention for CoV detection in Cynomops sp, a potential new reservoir. The phylogenetic analyses indicate that diversity of CoV in bats is higher than previously known.

Links

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Authors+Show Affiliations

Asano KM
Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil. karen.asano@gmail.com. Departament of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, Av. Orlando Marques de Paiva, 87, CEP: 05508-270, São Paulo, Brazil. karen.asano@gmail.com.
Hora AS
Departament of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, Av. Orlando Marques de Paiva, 87, CEP: 05508-270, São Paulo, Brazil.
Scheffer KC
Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil.
Fahl WO
Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil.
Iamamoto K
Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil.
Mori E
Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil.
Brandão PE
Departament of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, Av. Orlando Marques de Paiva, 87, CEP: 05508-270, São Paulo, Brazil.

MeSH

AnimalsBrazilChiropteraCoronavirusCoronavirus InfectionsGenome, ViralPhylogeny

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27342195

Citation

Asano, Karen Miyuki, et al. "Alphacoronavirus in Urban Molossidae and Phyllostomidae Bats, Brazil." Virology Journal, vol. 13, 2016, p. 110.
Asano KM, Hora AS, Scheffer KC, et al. Alphacoronavirus in urban Molossidae and Phyllostomidae bats, Brazil. Virol J. 2016;13:110.
Asano, K. M., Hora, A. S., Scheffer, K. C., Fahl, W. O., Iamamoto, K., Mori, E., & Brandão, P. E. (2016). Alphacoronavirus in urban Molossidae and Phyllostomidae bats, Brazil. Virology Journal, 13, 110. https://doi.org/10.1186/s12985-016-0569-4
Asano KM, et al. Alphacoronavirus in Urban Molossidae and Phyllostomidae Bats, Brazil. Virol J. 2016 06 24;13:110. PubMed PMID: 27342195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alphacoronavirus in urban Molossidae and Phyllostomidae bats, Brazil. AU - Asano,Karen Miyuki, AU - Hora,Aline Santana, AU - Scheffer,Karin Côrrea, AU - Fahl,Willian Oliveira, AU - Iamamoto,Keila, AU - Mori,Enio, AU - Brandão,Paulo Eduardo, Y1 - 2016/06/24/ PY - 2016/04/20/received PY - 2016/06/21/accepted PY - 2016/6/26/entrez PY - 2016/6/28/pubmed PY - 2017/8/2/medline KW - Bat KW - Coronavirus KW - Molossidae KW - Phyllostomidae SP - 110 EP - 110 JF - Virology journal JO - Virol J VL - 13 N2 - BACKGROUND: Bats have been implicated as the main reservoir of coronavirus (CoV). Thus the role of these hosts on the evolution and spread of CoVs currently deserve the attention of emerging diseases surveillance programs. On the view of the interest on and importance of CoVs in bats the occurrence and molecular characterization of CoV were conducted in bats from Brazil. FINDINGS: Three hundred five enteric contents of 29 bat species were tested using a panCoV nested RT-PCR. Nine specimens were positive and eight was suitable for RdRp gene sequencing. RdRp gene phylogeny showed that all CoVs strains from this study cluster in Alphacoronavirus genus, with one Molossidae and one Phlyllostomidae-CoV specific groups. Phylogenetic analyses of two S gene sequences showed a large diversity within the Alphacoronavirus genus. CONCLUSIONS: This study indicated a CoV-to-host specificity and draws attention for CoV detection in Cynomops sp, a potential new reservoir. The phylogenetic analyses indicate that diversity of CoV in bats is higher than previously known. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/27342195/Alphacoronavirus_in_urban_Molossidae_and_Phyllostomidae_bats_Brazil_ DB - PRIME DP - Unbound Medicine ER -