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Silver Nanolabels-Assisted Ion-Exchange Reaction with CdTe Quantum Dots Mediated Exciton Trapping for Signal-On Photoelectrochemical Immunoassay of Mycotoxins.
Anal Chem. 2016 08 02; 88(15):7858-66.AC

Abstract

Mycotoxins, highly toxic secondary metabolites produced by many invading species of filamentous fungi, contaminate different agricultural commodities under favorable temperature and humidity conditions. Herein, we successfully devised a novel signal-on photoelectrochemical immunosensing platform for the quantitative monitoring of mycotoxins (aflatoxin B1, AFB1, used as a model) in foodstuffs on the basis of silver nanolabels-assisted ion-exchange reaction with CdTe quantum dots (QDs) mediated hole-trapping. Initially, a competitive-type immunoreaction was carried out on a high-binding microplate by using silver nanoparticle (AgNP)-labeled AFB1-bovine serum albumin (AFB1-BSA) conjugates as the tags. Then, the carried AgNPs with AFB1-BSA were dissolved by acid to release numerous silver ions, which could induce ion-exchange reaction with the CdTe QDs immobilized on the electrode, thus resulting in formation of surface exciton trapping. Relative to pure CdTe QDs, the formed exciton trapping decreased the photocurrent of the modified electrode. In contrast, the detectable photocurrent increased with the increase of target AFB1 in a dynamic working range from 10 pg mL(-1) to 15 ng mL(-1) at a low limit of detection (LOD) of 3.0 pg mL(-1) under optimal conditions. In addition, the as-prepared photoelectrochemical immunosensing platform also displayed high specificity, good reproducibility, and acceptable method accuracy for detecting naturally contaminated/spiked blank peanut samples with consistent results obtained from the referenced enzyme-linked immunosorbent assay (ELISA) method.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Lin Y
Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province), Department of Chemistry, Fuzhou University , Fuzhou, Fujian 350108, People's Republic of China.
Zhou Q
Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province), Department of Chemistry, Fuzhou University , Fuzhou, Fujian 350108, People's Republic of China.
Tang D
Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province), Department of Chemistry, Fuzhou University , Fuzhou, Fujian 350108, People's Republic of China.
Niessner R
Chair for Analytical Chemistry, Institute of Hydrochemistry, Technische Universität München , Marchioninistrasse 17, D-81377 München, Germany.
Yang H
Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province), Department of Chemistry, Fuzhou University , Fuzhou, Fujian 350108, People's Republic of China.
Knopp D
Chair for Analytical Chemistry, Institute of Hydrochemistry, Technische Universität München , Marchioninistrasse 17, D-81377 München, Germany.

MeSH

Aflatoxin B1AnimalsCadmium CompoundsCattleElectrochemical TechniquesElectrodesImmunoassayLightLimit of DetectionMetal NanoparticlesMycotoxinsQuantum DotsSerum AlbuminSerum Albumin, BovineSilverTellurium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27348353

Citation

Lin, Youxiu, et al. "Silver Nanolabels-Assisted Ion-Exchange Reaction With CdTe Quantum Dots Mediated Exciton Trapping for Signal-On Photoelectrochemical Immunoassay of Mycotoxins." Analytical Chemistry, vol. 88, no. 15, 2016, pp. 7858-66.
Lin Y, Zhou Q, Tang D, et al. Silver Nanolabels-Assisted Ion-Exchange Reaction with CdTe Quantum Dots Mediated Exciton Trapping for Signal-On Photoelectrochemical Immunoassay of Mycotoxins. Anal Chem. 2016;88(15):7858-66.
Lin, Y., Zhou, Q., Tang, D., Niessner, R., Yang, H., & Knopp, D. (2016). Silver Nanolabels-Assisted Ion-Exchange Reaction with CdTe Quantum Dots Mediated Exciton Trapping for Signal-On Photoelectrochemical Immunoassay of Mycotoxins. Analytical Chemistry, 88(15), 7858-66. https://doi.org/10.1021/acs.analchem.6b02124
Lin Y, et al. Silver Nanolabels-Assisted Ion-Exchange Reaction With CdTe Quantum Dots Mediated Exciton Trapping for Signal-On Photoelectrochemical Immunoassay of Mycotoxins. Anal Chem. 2016 08 2;88(15):7858-66. PubMed PMID: 27348353.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silver Nanolabels-Assisted Ion-Exchange Reaction with CdTe Quantum Dots Mediated Exciton Trapping for Signal-On Photoelectrochemical Immunoassay of Mycotoxins. AU - Lin,Youxiu, AU - Zhou,Qian, AU - Tang,Dianping, AU - Niessner,Reinhard, AU - Yang,Huanghao, AU - Knopp,Dietmar, Y1 - 2016/07/11/ PY - 2016/6/28/entrez PY - 2016/6/28/pubmed PY - 2018/8/10/medline SP - 7858 EP - 66 JF - Analytical chemistry JO - Anal Chem VL - 88 IS - 15 N2 - Mycotoxins, highly toxic secondary metabolites produced by many invading species of filamentous fungi, contaminate different agricultural commodities under favorable temperature and humidity conditions. Herein, we successfully devised a novel signal-on photoelectrochemical immunosensing platform for the quantitative monitoring of mycotoxins (aflatoxin B1, AFB1, used as a model) in foodstuffs on the basis of silver nanolabels-assisted ion-exchange reaction with CdTe quantum dots (QDs) mediated hole-trapping. Initially, a competitive-type immunoreaction was carried out on a high-binding microplate by using silver nanoparticle (AgNP)-labeled AFB1-bovine serum albumin (AFB1-BSA) conjugates as the tags. Then, the carried AgNPs with AFB1-BSA were dissolved by acid to release numerous silver ions, which could induce ion-exchange reaction with the CdTe QDs immobilized on the electrode, thus resulting in formation of surface exciton trapping. Relative to pure CdTe QDs, the formed exciton trapping decreased the photocurrent of the modified electrode. In contrast, the detectable photocurrent increased with the increase of target AFB1 in a dynamic working range from 10 pg mL(-1) to 15 ng mL(-1) at a low limit of detection (LOD) of 3.0 pg mL(-1) under optimal conditions. In addition, the as-prepared photoelectrochemical immunosensing platform also displayed high specificity, good reproducibility, and acceptable method accuracy for detecting naturally contaminated/spiked blank peanut samples with consistent results obtained from the referenced enzyme-linked immunosorbent assay (ELISA) method. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/27348353/Silver_Nanolabels_Assisted_Ion_Exchange_Reaction_with_CdTe_Quantum_Dots_Mediated_Exciton_Trapping_for_Signal_On_Photoelectrochemical_Immunoassay_of_Mycotoxins_ DB - PRIME DP - Unbound Medicine ER -