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Sensory systems and ionocytes are targets for silver nanoparticle effects in fish.
Nanotoxicology. 2016 11; 10(9):1276-86.N

Abstract

Some nanoparticles (NPs) may induce adverse health effects in exposed organisms, but to date the evidence for this in wildlife is very limited. Silver nanoparticles (AgNPs) can be toxic to aquatic organisms, including fish, at concentrations relevant for some environmental exposures. We applied whole mount in-situ hybridisation (WISH) in zebrafish embryos and larvae for a suite of genes involved with detoxifying processes and oxidative stress, including metallothionein (mt2), glutathionine S-transferase pi (gstp), glutathionine S-transferase mu (gstm1), haem oxygenase (hmox1) and ferritin heavy chain 1 (fth1) to identify potential target tissues and effect mechanisms of AgNPs compared with a bulk counterpart and ionic silver (AgNO3). AgNPs caused upregulation in the expression of mt2, gstp and gstm1 and down regulation of expression of both hmox1 and fth1 and there were both life stage and tissue-specific responses. Responding tissues included olfactory bulbs, lateral line neuromasts and ionocytes in the skin with the potential for effects on olfaction, behaviour and maintenance of ion balance. Silver ions induced similar gene responses and affected the same target tissues as AgNPs. AgNPs invoked levels of target gene responses more similar to silver treatments compared to coated AgNPs indicating the responses seen were due to released silver ions. In the Nrf2 zebrafish mutant, expression of mt2 (24 hpf) and gstp (3 dpf) were either non-detectable or were at lower levels compared with wild type zebrafish for exposures to AgNPs, indicating that these gene responses are controlled through the Nrf2-Keap pathway.

Authors+Show Affiliations

a Department of Biosciences , College of Life and Environmental Sciences, University of Exeter , Exeter , UK .b Faculty of Medicine , Molecular and Developmental Biology, University of Tsukuba , Tsukuba , Japan .b Faculty of Medicine , Molecular and Developmental Biology, University of Tsukuba , Tsukuba , Japan .c Department of Earth and Environmental Sciences , School of Geography, University of Birmingham , Birmingham , UK , and.c Department of Earth and Environmental Sciences , School of Geography, University of Birmingham , Birmingham , UK , and.c Department of Earth and Environmental Sciences , School of Geography, University of Birmingham , Birmingham , UK , and.c Department of Earth and Environmental Sciences , School of Geography, University of Birmingham , Birmingham , UK , and.a Department of Biosciences , College of Life and Environmental Sciences, University of Exeter , Exeter , UK .d Department of Biological Sciences , Tokyo Institute of Technology , Yokohama , Japan.c Department of Earth and Environmental Sciences , School of Geography, University of Birmingham , Birmingham , UK , and.b Faculty of Medicine , Molecular and Developmental Biology, University of Tsukuba , Tsukuba , Japan .a Department of Biosciences , College of Life and Environmental Sciences, University of Exeter , Exeter , UK .a Department of Biosciences , College of Life and Environmental Sciences, University of Exeter , Exeter , UK .

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27350075

Citation

Osborne, Olivia J., et al. "Sensory Systems and Ionocytes Are Targets for Silver Nanoparticle Effects in Fish." Nanotoxicology, vol. 10, no. 9, 2016, pp. 1276-86.
Osborne OJ, Mukaigasa K, Nakajima H, et al. Sensory systems and ionocytes are targets for silver nanoparticle effects in fish. Nanotoxicology. 2016;10(9):1276-86.
Osborne, O. J., Mukaigasa, K., Nakajima, H., Stolpe, B., Romer, I., Philips, U., Lynch, I., Mourabit, S., Hirose, S., Lead, J. R., Kobayashi, M., Kudoh, T., & Tyler, C. R. (2016). Sensory systems and ionocytes are targets for silver nanoparticle effects in fish. Nanotoxicology, 10(9), 1276-86. https://doi.org/10.1080/17435390.2016.1206147
Osborne OJ, et al. Sensory Systems and Ionocytes Are Targets for Silver Nanoparticle Effects in Fish. Nanotoxicology. 2016;10(9):1276-86. PubMed PMID: 27350075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensory systems and ionocytes are targets for silver nanoparticle effects in fish. AU - Osborne,Olivia J, AU - Mukaigasa,Katsuki, AU - Nakajima,Hitomi, AU - Stolpe,Bjorn, AU - Romer,Isabella, AU - Philips,Uzoma, AU - Lynch,Iseult, AU - Mourabit,Sulayman, AU - Hirose,Shigehisa, AU - Lead,Jamie R, AU - Kobayashi,Makoto, AU - Kudoh,Tetsuhiro, AU - Tyler,Charles R, Y1 - 2016/07/18/ PY - 2016/6/29/entrez PY - 2016/6/29/pubmed PY - 2017/6/16/medline KW - Danio rerio KW - Nrf2 pathway KW - oxidative stress KW - silver nanoparticles KW - target tissues SP - 1276 EP - 86 JF - Nanotoxicology JO - Nanotoxicology VL - 10 IS - 9 N2 - Some nanoparticles (NPs) may induce adverse health effects in exposed organisms, but to date the evidence for this in wildlife is very limited. Silver nanoparticles (AgNPs) can be toxic to aquatic organisms, including fish, at concentrations relevant for some environmental exposures. We applied whole mount in-situ hybridisation (WISH) in zebrafish embryos and larvae for a suite of genes involved with detoxifying processes and oxidative stress, including metallothionein (mt2), glutathionine S-transferase pi (gstp), glutathionine S-transferase mu (gstm1), haem oxygenase (hmox1) and ferritin heavy chain 1 (fth1) to identify potential target tissues and effect mechanisms of AgNPs compared with a bulk counterpart and ionic silver (AgNO3). AgNPs caused upregulation in the expression of mt2, gstp and gstm1 and down regulation of expression of both hmox1 and fth1 and there were both life stage and tissue-specific responses. Responding tissues included olfactory bulbs, lateral line neuromasts and ionocytes in the skin with the potential for effects on olfaction, behaviour and maintenance of ion balance. Silver ions induced similar gene responses and affected the same target tissues as AgNPs. AgNPs invoked levels of target gene responses more similar to silver treatments compared to coated AgNPs indicating the responses seen were due to released silver ions. In the Nrf2 zebrafish mutant, expression of mt2 (24 hpf) and gstp (3 dpf) were either non-detectable or were at lower levels compared with wild type zebrafish for exposures to AgNPs, indicating that these gene responses are controlled through the Nrf2-Keap pathway. SN - 1743-5404 UR - https://www.unboundmedicine.com/medline/citation/27350075/Sensory_systems_and_ionocytes_are_targets_for_silver_nanoparticle_effects_in_fish_ L2 - https://www.tandfonline.com/doi/full/10.1080/17435390.2016.1206147 DB - PRIME DP - Unbound Medicine ER -