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In silico evaluation of inhibitory potential of triterpenoids from Azadirachta indica against therapeutic target of dengue virus, NS2B-NS3 protease.
J Vector Borne Dis. 2016 Apr-Jun; 53(2):156-61.JV

Abstract

BACKGROUND & OBJECTIVES

NS2B-NS3 protease (NS2B-NS3 pro) of dengue virus (DENV) is the prime therapeutic target for the development of anti-dengue drug to combat the DENV infection, which is currently an increasing health problem in many countries. In the area of antiviral drug discovery, numerous reports on the antiviral activity of various medicinal plants against dengue viruses have been published. Neem plant (Azadirachta indica) is one among those medicinal plants which is reported to show potential antiviral activity against DENV. But active principle of neem plant extract which has inhibitory potential against DENV NS2B-NS3 pro is not yet reported. The aim of the present study was to explore the inhibitory potential of five triterpenoids from neem plant, viz. nimbin, desacetylnimbin, desacetylsalannin, azadirachtin and salannin, against DENV NS2B-NS3 pro.

METHODS

The molecular 3D structural data of DENV NS2B-NS3 pro and selected triterpenoids of neem plant were collected from protein databank (PDB ID: 2VBC) and PubChem database respectively. The molecular docking approach was employed to find out the in silico inhibitory potential of the five triterpenoids against DENV NS2B- NS3 pro.

RESULTS

The molecular docking results showed that nimbin, desacetylnimbin and desacetylsalannin have good binding affinity with DENV NS2B-NS3 pro , while azadirachtin and salannin did not show any interaction with the target protein. It was observed that the DENV NS2B-NS3 pro binding energy for nimbin, desacetylnimbin and desacetylsalannin were -5.56, -5.24 and -3.43 kcal/mol, respectively.

INTERPRETATION & CONCLUSION

The findings attained through this study on the molecular interaction mode of three neem triterpenoids and DENV NS2B-NS3 pro can be considered for further in vitro and in vivo validation for designing new potential drugs for DENV infection.

Authors+Show Affiliations

Dwivedi VD
Faculty of Science and Environment, Mahatma Gandhi Chitrakoot Gramodaya Vishwavidyalaya, Chitrakoot, Satna, Madhya Pradesh; Department of Biotechnology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, Uttar Pradesh, India.
Tripathi IP
Faculty of Science and Environment, Mahatma Gandhi Chitrakoot Gramodaya Vishwavidyalaya, Chitrakoot, Satna, Madhya Pradesh, India.
Mishra SK
Department of Biotechnology, Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, Uttar Pradesh, India.

MeSH

AnimalsAntiviral AgentsAzadirachtaComputer SimulationDengue VirusModels, MolecularMolecular Docking SimulationPhytochemicalsProtease InhibitorsProtein ConformationRNA HelicasesSerine EndopeptidasesTriterpenesViral Nonstructural Proteins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27353586

Citation

Dwivedi, Vivek Dhar, et al. "In Silico Evaluation of Inhibitory Potential of Triterpenoids From Azadirachta Indica Against Therapeutic Target of Dengue Virus, NS2B-NS3 Protease." Journal of Vector Borne Diseases, vol. 53, no. 2, 2016, pp. 156-61.
Dwivedi VD, Tripathi IP, Mishra SK. In silico evaluation of inhibitory potential of triterpenoids from Azadirachta indica against therapeutic target of dengue virus, NS2B-NS3 protease. J Vector Borne Dis. 2016;53(2):156-61.
Dwivedi, V. D., Tripathi, I. P., & Mishra, S. K. (2016). In silico evaluation of inhibitory potential of triterpenoids from Azadirachta indica against therapeutic target of dengue virus, NS2B-NS3 protease. Journal of Vector Borne Diseases, 53(2), 156-61.
Dwivedi VD, Tripathi IP, Mishra SK. In Silico Evaluation of Inhibitory Potential of Triterpenoids From Azadirachta Indica Against Therapeutic Target of Dengue Virus, NS2B-NS3 Protease. J Vector Borne Dis. 2016 Apr-Jun;53(2):156-61. PubMed PMID: 27353586.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In silico evaluation of inhibitory potential of triterpenoids from Azadirachta indica against therapeutic target of dengue virus, NS2B-NS3 protease. AU - Dwivedi,Vivek Dhar, AU - Tripathi,Indra Prasad, AU - Mishra,Sarad Kumar, PY - 2016/6/30/entrez PY - 2016/6/30/pubmed PY - 2017/4/6/medline SP - 156 EP - 61 JF - Journal of vector borne diseases JO - J Vector Borne Dis VL - 53 IS - 2 N2 - BACKGROUND & OBJECTIVES: NS2B-NS3 protease (NS2B-NS3 pro) of dengue virus (DENV) is the prime therapeutic target for the development of anti-dengue drug to combat the DENV infection, which is currently an increasing health problem in many countries. In the area of antiviral drug discovery, numerous reports on the antiviral activity of various medicinal plants against dengue viruses have been published. Neem plant (Azadirachta indica) is one among those medicinal plants which is reported to show potential antiviral activity against DENV. But active principle of neem plant extract which has inhibitory potential against DENV NS2B-NS3 pro is not yet reported. The aim of the present study was to explore the inhibitory potential of five triterpenoids from neem plant, viz. nimbin, desacetylnimbin, desacetylsalannin, azadirachtin and salannin, against DENV NS2B-NS3 pro. METHODS: The molecular 3D structural data of DENV NS2B-NS3 pro and selected triterpenoids of neem plant were collected from protein databank (PDB ID: 2VBC) and PubChem database respectively. The molecular docking approach was employed to find out the in silico inhibitory potential of the five triterpenoids against DENV NS2B- NS3 pro. RESULTS: The molecular docking results showed that nimbin, desacetylnimbin and desacetylsalannin have good binding affinity with DENV NS2B-NS3 pro , while azadirachtin and salannin did not show any interaction with the target protein. It was observed that the DENV NS2B-NS3 pro binding energy for nimbin, desacetylnimbin and desacetylsalannin were -5.56, -5.24 and -3.43 kcal/mol, respectively. INTERPRETATION & CONCLUSION: The findings attained through this study on the molecular interaction mode of three neem triterpenoids and DENV NS2B-NS3 pro can be considered for further in vitro and in vivo validation for designing new potential drugs for DENV infection. SN - 0972-9062 UR - https://www.unboundmedicine.com/medline/citation/27353586/In_silico_evaluation_of_inhibitory_potential_of_triterpenoids_from_Azadirachta_indica_against_therapeutic_target_of_dengue_virus_NS2B_NS3_protease_ DB - PRIME DP - Unbound Medicine ER -