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Natural water as the test medium for Ag and CuO nanoparticle hazard evaluation: An interlaboratory case study.
Environ Pollut. 2016 Sep; 216:689-699.EP

Abstract

Engineered nanoparticles (NPs) have realistic potential of reaching natural waterbodies and of exerting toxicity to freshwater organisms. The toxicity may be influenced by the composition of natural waters as crucial NP properties are influenced by water constituents. To tackle this issue, a case study was set up in the framework of EU FP7 NanoValid project, performing an interlaboratory hazard evaluation of NPs in natural freshwater. Ag and CuO NPs were selected as model NPs because of their potentially high toxicity in the freshwater. Daphnia magna (OECD202) and Danio rerio embryo (OECD236) assays were used to evaluate NP toxicity in natural water, sampled from Lake Greifen and Lake Lucerne (Switzerland). Dissolution of the NPs was evaluated by ultrafiltration, ultracentrifugation and metal specific sensor bacteria. Ag NP size was stable in natural water while CuO NPs agglomerated and settled rapidly. Ag NP suspensions contained a large fraction of Ag(+) ions and CuO NP suspensions had low concentration of Cu(2+) ions. Ag NPs were very toxic (48 h EC50 1-5.5 μg Ag/L) to D. magna as well as to D. rerio embryos (96 h EC50 8.8-61 μg Ag/L) in both standard media and natural waters with results in good agreement between laboratories. CuO NP toxicity to D. magna differed significantly between the laboratories with 48 h EC50 0.9-11 mg Cu/L in standard media, 5.7-75 mg Cu/L in Lake Greifen and 5.5-26 mg Cu/L in Lake Lucerne. No toxicity of CuO NP to zebrafish embryos was detected up to 100 mg/L independent of the medium used. The results show that Ag and CuO NP toxicity may be higher in natural water than in the standard media due to differences in composition. NP environmental hazard evaluation can and should be carried out in natural water to obtain more realistic estimates on the toxicity.

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Authors+Show Affiliations

Heinlaan M
Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia. Electronic address: margit.heinlaan@kbfi.ee.
Muna M
Laboratory of Environmental Toxicology, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia; Department of Materials Science, Tallinn University of Technology, Ehitajate tee 5, 19086 Tallinn, Estonia.
Knöbel M
Eawag, Swiss Federal Institute of Aquatic Science and Technology, Überlandstrasse 133, 8600 Dübendorf, Switzerland.
Kistler D
Eawag, Swiss Federal Institute of Aquatic Science and Technology, Überlandstrasse 133, 8600 Dübendorf, Switzerland.
Odzak N
Eawag, Swiss Federal Institute of Aquatic Science and Technology, Überlandstrasse 133, 8600 Dübendorf, Switzerland.
Kühnel D
Helmholtz Centre for Environmental Research - UFZ, Department of Bioanalytical Ecotoxicology, Permoserstr.15, 04318 Leipzig, Germany.
Müller J
Helmholtz Centre for Environmental Research - UFZ, Department of Bioanalytical Ecotoxicology, Permoserstr.15, 04318 Leipzig, Germany.
Gupta GS
Institute of Life Sciences, School of Science and Technology, Ahmedabad University, University Road, Navrangpura, Ahmedabad, 380009, Gujarat, India.
Kumar A
Institute of Life Sciences, School of Science and Technology, Ahmedabad University, University Road, Navrangpura, Ahmedabad, 380009, Gujarat, India.
Shanker R
Institute of Life Sciences, School of Science and Technology, Ahmedabad University, University Road, Navrangpura, Ahmedabad, 380009, Gujarat, India.
Sigg L
Eawag, Swiss Federal Institute of Aquatic Science and Technology, Überlandstrasse 133, 8600 Dübendorf, Switzerland. Electronic address: laura.sigg@eawag.ch.

MeSH

AnimalsCopperDaphniaHazardous SubstancesLakesMetal NanoparticlesSilverSwitzerlandToxicity TestsWater Pollutants, ChemicalZebrafish

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27357482

Citation

Heinlaan, Margit, et al. "Natural Water as the Test Medium for Ag and CuO Nanoparticle Hazard Evaluation: an Interlaboratory Case Study." Environmental Pollution (Barking, Essex : 1987), vol. 216, 2016, pp. 689-699.
Heinlaan M, Muna M, Knöbel M, et al. Natural water as the test medium for Ag and CuO nanoparticle hazard evaluation: An interlaboratory case study. Environ Pollut. 2016;216:689-699.
Heinlaan, M., Muna, M., Knöbel, M., Kistler, D., Odzak, N., Kühnel, D., Müller, J., Gupta, G. S., Kumar, A., Shanker, R., & Sigg, L. (2016). Natural water as the test medium for Ag and CuO nanoparticle hazard evaluation: An interlaboratory case study. Environmental Pollution (Barking, Essex : 1987), 216, 689-699. https://doi.org/10.1016/j.envpol.2016.06.033
Heinlaan M, et al. Natural Water as the Test Medium for Ag and CuO Nanoparticle Hazard Evaluation: an Interlaboratory Case Study. Environ Pollut. 2016;216:689-699. PubMed PMID: 27357482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Natural water as the test medium for Ag and CuO nanoparticle hazard evaluation: An interlaboratory case study. AU - Heinlaan,Margit, AU - Muna,Marge, AU - Knöbel,Melanie, AU - Kistler,David, AU - Odzak,Niksa, AU - Kühnel,Dana, AU - Müller,Josefine, AU - Gupta,Govind Sharan, AU - Kumar,Ashutosh, AU - Shanker,Rishi, AU - Sigg,Laura, Y1 - 2016/06/27/ PY - 2016/04/15/received PY - 2016/06/15/revised PY - 2016/06/16/accepted PY - 2016/7/1/entrez PY - 2016/7/1/pubmed PY - 2017/1/7/medline KW - Danio rerio KW - Daphnia magna KW - Environmentally relevant conditions KW - Freshwater KW - NanoValid KW - Zebrafish SP - 689 EP - 699 JF - Environmental pollution (Barking, Essex : 1987) JO - Environ Pollut VL - 216 N2 - Engineered nanoparticles (NPs) have realistic potential of reaching natural waterbodies and of exerting toxicity to freshwater organisms. The toxicity may be influenced by the composition of natural waters as crucial NP properties are influenced by water constituents. To tackle this issue, a case study was set up in the framework of EU FP7 NanoValid project, performing an interlaboratory hazard evaluation of NPs in natural freshwater. Ag and CuO NPs were selected as model NPs because of their potentially high toxicity in the freshwater. Daphnia magna (OECD202) and Danio rerio embryo (OECD236) assays were used to evaluate NP toxicity in natural water, sampled from Lake Greifen and Lake Lucerne (Switzerland). Dissolution of the NPs was evaluated by ultrafiltration, ultracentrifugation and metal specific sensor bacteria. Ag NP size was stable in natural water while CuO NPs agglomerated and settled rapidly. Ag NP suspensions contained a large fraction of Ag(+) ions and CuO NP suspensions had low concentration of Cu(2+) ions. Ag NPs were very toxic (48 h EC50 1-5.5 μg Ag/L) to D. magna as well as to D. rerio embryos (96 h EC50 8.8-61 μg Ag/L) in both standard media and natural waters with results in good agreement between laboratories. CuO NP toxicity to D. magna differed significantly between the laboratories with 48 h EC50 0.9-11 mg Cu/L in standard media, 5.7-75 mg Cu/L in Lake Greifen and 5.5-26 mg Cu/L in Lake Lucerne. No toxicity of CuO NP to zebrafish embryos was detected up to 100 mg/L independent of the medium used. The results show that Ag and CuO NP toxicity may be higher in natural water than in the standard media due to differences in composition. NP environmental hazard evaluation can and should be carried out in natural water to obtain more realistic estimates on the toxicity. SN - 1873-6424 UR - https://www.unboundmedicine.com/medline/citation/27357482/Natural_water_as_the_test_medium_for_Ag_and_CuO_nanoparticle_hazard_evaluation:_An_interlaboratory_case_study_ DB - PRIME DP - Unbound Medicine ER -