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Baicalein modulates stress-resistance and life span in C. elegans via SKN-1 but not DAF-16.
Fitoterapia 2016; 113:123-7F

Abstract

The flavonoid baicalein has been demonstrated to be an activator of the transcription factor Nrf2 in mammalian cell lines. We show that it further modulates the Nrf2 homolog SKN-1 in Caenorhabditis elegans and by this pathway mediates beneficial effects in the nematode: baicalein enhances the resistance of C. elegans against lethal thermal and sodium arsenite stress and dose-dependently prolongs the life span of the nematode. Using RNA interference against SKN-1 we were able to show that the induction of longevity and the enhanced stress-resistance were dependent on this transcription factor. DAF-16 (homolog to mammalian FOXO) is another pivotal aging-related transcription factor in the nematode. We demonstrate that DAF-16 does not participate in the beneficial effects of baicalein: since baicalein causes no increase in the nuclear translocation of DAF-16 (DAF-16::GFP expressing strain, incubation time: 1h) and it still induces longevity even in a DAF-16 loss-of-function strain, we conclude, that baicalein increases stress-resistance and life span in C. elegans via SKN-1 but not DAF-16.

Authors+Show Affiliations

Martin-Luther-Universität Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Weinbergweg 22, 06120 Halle/Saale, Germany; Westfälische Wilhelms-Universität Münster, Institute of Food Chemistry, Corrensstr. 45, 48149 Münster, Germany; Heinrich-Heine-Universität Düsseldorf, Institute of Toxicology, P.O. Box 101007, 40001 Düsseldorf, Germany.Westfälische Wilhelms-Universität Münster, Institute of Food Chemistry, Corrensstr. 45, 48149 Münster, Germany.Martin-Luther-Universität Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences, Weinbergweg 22, 06120 Halle/Saale, Germany; Heinrich-Heine-Universität Düsseldorf, Institute of Toxicology, P.O. Box 101007, 40001 Düsseldorf, Germany. Electronic address: wim.waetjen@landw.uni-halle.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27370100

Citation

Havermann, Susannah, et al. "Baicalein Modulates Stress-resistance and Life Span in C. Elegans Via SKN-1 but Not DAF-16." Fitoterapia, vol. 113, 2016, pp. 123-7.
Havermann S, Humpf HU, Wätjen W. Baicalein modulates stress-resistance and life span in C. elegans via SKN-1 but not DAF-16. Fitoterapia. 2016;113:123-7.
Havermann, S., Humpf, H. U., & Wätjen, W. (2016). Baicalein modulates stress-resistance and life span in C. elegans via SKN-1 but not DAF-16. Fitoterapia, 113, pp. 123-7. doi:10.1016/j.fitote.2016.06.018.
Havermann S, Humpf HU, Wätjen W. Baicalein Modulates Stress-resistance and Life Span in C. Elegans Via SKN-1 but Not DAF-16. Fitoterapia. 2016;113:123-7. PubMed PMID: 27370100.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Baicalein modulates stress-resistance and life span in C. elegans via SKN-1 but not DAF-16. AU - Havermann,Susannah, AU - Humpf,Hans-Ulrich, AU - Wätjen,Wim, Y1 - 2016/06/28/ PY - 2015/06/08/received PY - 2016/06/24/revised PY - 2016/06/27/accepted PY - 2016/7/3/entrez PY - 2016/7/3/pubmed PY - 2017/2/1/medline KW - Insulin-signaling KW - Nrf2 KW - Oxidative stress KW - Secondary plant compounds KW - TCM SP - 123 EP - 7 JF - Fitoterapia JO - Fitoterapia VL - 113 N2 - The flavonoid baicalein has been demonstrated to be an activator of the transcription factor Nrf2 in mammalian cell lines. We show that it further modulates the Nrf2 homolog SKN-1 in Caenorhabditis elegans and by this pathway mediates beneficial effects in the nematode: baicalein enhances the resistance of C. elegans against lethal thermal and sodium arsenite stress and dose-dependently prolongs the life span of the nematode. Using RNA interference against SKN-1 we were able to show that the induction of longevity and the enhanced stress-resistance were dependent on this transcription factor. DAF-16 (homolog to mammalian FOXO) is another pivotal aging-related transcription factor in the nematode. We demonstrate that DAF-16 does not participate in the beneficial effects of baicalein: since baicalein causes no increase in the nuclear translocation of DAF-16 (DAF-16::GFP expressing strain, incubation time: 1h) and it still induces longevity even in a DAF-16 loss-of-function strain, we conclude, that baicalein increases stress-resistance and life span in C. elegans via SKN-1 but not DAF-16. SN - 1873-6971 UR - https://www.unboundmedicine.com/medline/citation/27370100/Baicalein_modulates_stress_resistance_and_life_span_in_C__elegans_via_SKN_1_but_not_DAF_16_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0367-326X(16)30142-3 DB - PRIME DP - Unbound Medicine ER -