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Role of the yeast ribosomal protein L16 in ribosome biogenesis.
FEBS J. 2016 08; 283(16):2968-85.FJ

Abstract

Most ribosomal proteins play essential roles in ribosome synthesis and function. In this study, we have analysed the contribution of yeast ribosomal protein L16 to ribosome biogenesis. We show that in vivo depletion of the essential L16 protein results in a deficit in 60S subunits and the appearance of half-mer polysomes. This phenotype is likely due to the instability and rapid turnover of early and intermediate pre-60S particles, as evidenced by the reduced steady-state levels of 27SBS and 7SL /S pre-rRNA, and the low amounts of de novo synthesized 27S pre-rRNA and 25S rRNA. Additionally, depletion of L16 blocks nucleocytoplasmic export of pre-60S particles. Moreover, we show that L16 assembles in the nucleolus and binds to early 90S preribosomal particles. Many evolutionarily conserved ribosomal proteins possess extra eukaryote-specific amino- or carboxy-terminal extensions and/or internal loops. Here, we have also investigated the role of the eukaryote-specific carboxy-terminal extension of L16. Progressive truncation of this extension recapitulates, albeit to a lesser extent, the growth and ribosome biogenesis defects of the L16 depletion. We conclude that L16 assembly is a prerequisite to properly stabilize rRNA structures within early pre-60S particles, thereby favouring efficient 27S pre-rRNA processing within the internal transcribed spacer 1 at sites A3 and B1 . Upon depletion of L16, the lack of this stabilization aborts early pre-60S particle assembly and subjects these intermediates to turnover.

Authors+Show Affiliations

Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla and Departamento de Genética, Universidad de Sevilla, Spain.Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla and Departamento de Genética, Universidad de Sevilla, Spain.Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla and Departamento de Genética, Universidad de Sevilla, Spain.Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla and Departamento de Genética, Universidad de Sevilla, Spain.Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla and Departamento de Genética, Universidad de Sevilla, Spain.Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla and Departamento de Genética, Universidad de Sevilla, Spain.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27374275

Citation

Espinar-Marchena, Francisco J., et al. "Role of the Yeast Ribosomal Protein L16 in Ribosome Biogenesis." The FEBS Journal, vol. 283, no. 16, 2016, pp. 2968-85.
Espinar-Marchena FJ, Fernández-Fernández J, Rodríguez-Galán O, et al. Role of the yeast ribosomal protein L16 in ribosome biogenesis. FEBS J. 2016;283(16):2968-85.
Espinar-Marchena, F. J., Fernández-Fernández, J., Rodríguez-Galán, O., Fernández-Pevida, A., Babiano, R., & de la Cruz, J. (2016). Role of the yeast ribosomal protein L16 in ribosome biogenesis. The FEBS Journal, 283(16), 2968-85. https://doi.org/10.1111/febs.13797
Espinar-Marchena FJ, et al. Role of the Yeast Ribosomal Protein L16 in Ribosome Biogenesis. FEBS J. 2016;283(16):2968-85. PubMed PMID: 27374275.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of the yeast ribosomal protein L16 in ribosome biogenesis. AU - Espinar-Marchena,Francisco J, AU - Fernández-Fernández,José, AU - Rodríguez-Galán,Olga, AU - Fernández-Pevida,Antonio, AU - Babiano,Reyes, AU - de la Cruz,Jesús, Y1 - 2016/07/15/ PY - 2016/03/05/received PY - 2016/06/02/revised PY - 2016/06/29/accepted PY - 2016/7/5/entrez PY - 2016/7/5/pubmed PY - 2017/6/16/medline KW - Saccharomyces cerevisiae KW - nucleolus KW - pre-rRNA processing KW - ribosomal RNA KW - ribosomal protein L16 (uL13) KW - ribosome synthesis SP - 2968 EP - 85 JF - The FEBS journal JO - FEBS J. VL - 283 IS - 16 N2 - Most ribosomal proteins play essential roles in ribosome synthesis and function. In this study, we have analysed the contribution of yeast ribosomal protein L16 to ribosome biogenesis. We show that in vivo depletion of the essential L16 protein results in a deficit in 60S subunits and the appearance of half-mer polysomes. This phenotype is likely due to the instability and rapid turnover of early and intermediate pre-60S particles, as evidenced by the reduced steady-state levels of 27SBS and 7SL /S pre-rRNA, and the low amounts of de novo synthesized 27S pre-rRNA and 25S rRNA. Additionally, depletion of L16 blocks nucleocytoplasmic export of pre-60S particles. Moreover, we show that L16 assembles in the nucleolus and binds to early 90S preribosomal particles. Many evolutionarily conserved ribosomal proteins possess extra eukaryote-specific amino- or carboxy-terminal extensions and/or internal loops. Here, we have also investigated the role of the eukaryote-specific carboxy-terminal extension of L16. Progressive truncation of this extension recapitulates, albeit to a lesser extent, the growth and ribosome biogenesis defects of the L16 depletion. We conclude that L16 assembly is a prerequisite to properly stabilize rRNA structures within early pre-60S particles, thereby favouring efficient 27S pre-rRNA processing within the internal transcribed spacer 1 at sites A3 and B1 . Upon depletion of L16, the lack of this stabilization aborts early pre-60S particle assembly and subjects these intermediates to turnover. SN - 1742-4658 UR - https://www.unboundmedicine.com/medline/citation/27374275/Role_of_the_yeast_ribosomal_protein_L16_in_ribosome_biogenesis_ L2 - https://doi.org/10.1111/febs.13797 DB - PRIME DP - Unbound Medicine ER -