Prevalence and behavior of multidrug-resistant shiga toxin-producing Escherichia coli, enteropathogenic E. coli and enterotoxigenic E. coli on coriander.
Food Microbiol. 2016 Oct; 59:97-103.FM

Abstract

The prevalence and behavior of multidrug-resistant diarrheagenic Escherichia coli pathotypes on coriander was determined. One hundred coriander samples were collected from markets. Generic E. coli were determined using the most probable number procedure. Diarrheagenic E. coli pathotypes (DEPs) were identified using two multiplex polymerase chain reaction procedures. Susceptibility to sixteen antibiotics was tested for the isolated DEPs strains by standard test. The behavior of multidrug-resistant DEPs isolated from coriander was determined on coriander leaves and chopped coriander at 25°± 2 °C and 3°± 2 °C. Generic E. coli and DEPs were identified, respectively, in 43 and 7% of samples. Nine DEPs strains were isolated from positive coriander samples. The identified DEPs included Shiga toxin-producing E. coli (STEC, 4%) enterotoxigenic E. coli (ETEC, 2%) and enteropathogenic E. coli (EPEC, 1%). All isolated DEPs strains exhibited multi-resistance to antibiotics. On inoculated coriander leaves stored at 25°± 2 °C or 3°± 2 °C, no growth was observed for multidrug-resistant DEPs strains. However, multidrug-resistant DEPs strains grew in chopped coriander: after 24 h at 25° ± 2 °C, DEPs strains had grown to approximately 3 log CFU/g. However, at 3°± 2 °C the bacterial growth was inhibited. To the best of our knowledge, this is the first report of the presence and behavior of multidrug-resistant STEC, ETEC and EPEC on coriander and chopped coriander.

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Authors+Show Affiliations

Gómez-Aldapa CA

Centro de Investigaciones Químicas, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Ciudad del Conocimiento, Carretera Pachuca-Tulancingo Km. 4.5. 42184, Mineral de la Reforma, Hidalgo, Mexico.

Segovia-Cruz JA

Cerna-Cortes JF

Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas-IPN, Prolongación Carpio y Plan de Ayala s/n, Col. Casco de Santo Tomas, Del. Miguel Hidalgo, 11340, México, D.F., Mexico.

Rangel-Vargas E

Salas-Rangel LP

Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas-IPN, Prolongación Carpio y Plan de Ayala s/n, Col. Casco de Santo Tomas, Del. Miguel Hidalgo, 11340, México, D.F., Mexico.

Gutiérrez-Alcántara EJ

Castro-Rosas J

MeSH

Anti-Bacterial AgentsBacterial LoadCoriandrumDrug Resistance, Multiple, BacterialEnteropathogenic Escherichia coliEnterotoxigenic Escherichia coliFood ContaminationFood MicrobiologyMexicoPolymerase Chain ReactionShiga-Toxigenic Escherichia coliTemperature

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27375249

Citation

Gómez-Aldapa, Carlos A., et al. "Prevalence and Behavior of Multidrug-resistant Shiga Toxin-producing Escherichia Coli, Enteropathogenic E. Coli and Enterotoxigenic E. Coli On Coriander." Food Microbiology, vol. 59, 2016, pp. 97-103.

Gómez-Aldapa CA, Segovia-Cruz JA, Cerna-Cortes JF, et al. Prevalence and behavior of multidrug-resistant shiga toxin-producing Escherichia coli, enteropathogenic E. coli and enterotoxigenic E. coli on coriander. Food Microbiol. 2016;59:97-103.

Gómez-Aldapa, C. A., Segovia-Cruz, J. A., Cerna-Cortes, J. F., Rangel-Vargas, E., Salas-Rangel, L. P., Gutiérrez-Alcántara, E. J., & Castro-Rosas, J. (2016). Prevalence and behavior of multidrug-resistant shiga toxin-producing Escherichia coli, enteropathogenic E. coli and enterotoxigenic E. coli on coriander. Food Microbiology, 59, 97-103. https://doi.org/10.1016/j.fm.2016.05.014

Gómez-Aldapa CA, et al. Prevalence and Behavior of Multidrug-resistant Shiga Toxin-producing Escherichia Coli, Enteropathogenic E. Coli and Enterotoxigenic E. Coli On Coriander. Food Microbiol. 2016;59:97-103. PubMed PMID: 27375249.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Prevalence and behavior of multidrug-resistant shiga toxin-producing Escherichia coli, enteropathogenic E. coli and enterotoxigenic E. coli on coriander. AU - Gómez-Aldapa,Carlos A, AU - Segovia-Cruz,Jesús A, AU - Cerna-Cortes,Jorge F, AU - Rangel-Vargas,Esmeralda, AU - Salas-Rangel,Laura P, AU - Gutiérrez-Alcántara,Eduardo J, AU - Castro-Rosas,Javier, Y1 - 2016/05/28/ PY - 2015/09/14/received PY - 2016/05/23/revised PY - 2016/05/27/accepted PY - 2016/7/5/entrez PY - 2016/7/5/pubmed PY - 2017/3/1/medline KW - Antibiotic KW - Coriander KW - EPEC KW - ETEC KW - Multidrug-resistant KW - STEC SP - 97 EP - 103 JF - Food microbiology JO - Food Microbiol VL - 59 N2 - The prevalence and behavior of multidrug-resistant diarrheagenic Escherichia coli pathotypes on coriander was determined. One hundred coriander samples were collected from markets. Generic E. coli were determined using the most probable number procedure. Diarrheagenic E. coli pathotypes (DEPs) were identified using two multiplex polymerase chain reaction procedures. Susceptibility to sixteen antibiotics was tested for the isolated DEPs strains by standard test. The behavior of multidrug-resistant DEPs isolated from coriander was determined on coriander leaves and chopped coriander at 25°± 2 °C and 3°± 2 °C. Generic E. coli and DEPs were identified, respectively, in 43 and 7% of samples. Nine DEPs strains were isolated from positive coriander samples. The identified DEPs included Shiga toxin-producing E. coli (STEC, 4%) enterotoxigenic E. coli (ETEC, 2%) and enteropathogenic E. coli (EPEC, 1%). All isolated DEPs strains exhibited multi-resistance to antibiotics. On inoculated coriander leaves stored at 25°± 2 °C or 3°± 2 °C, no growth was observed for multidrug-resistant DEPs strains. However, multidrug-resistant DEPs strains grew in chopped coriander: after 24 h at 25° ± 2 °C, DEPs strains had grown to approximately 3 log CFU/g. However, at 3°± 2 °C the bacterial growth was inhibited. To the best of our knowledge, this is the first report of the presence and behavior of multidrug-resistant STEC, ETEC and EPEC on coriander and chopped coriander. SN - 1095-9998 UR - https://www.unboundmedicine.com/medline/citation/27375249/Prevalence_and_behavior_of_multidrug_resistant_shiga_toxin_producing_Escherichia_coli_enteropathogenic_E__coli_and_enterotoxigenic_E__coli_on_coriander_ DB - PRIME DP - Unbound Medicine ER -

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Prevalence and behavior of multidrug-resistant shiga toxin-producing Escherichia coli, enteropathogenic E. coli and enterotoxigenic E. coli on coriander.Food Microbiol. 2016 Oct; 59:97-103.FM