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Preclinical evaluation of three polyspecific antivenoms against the venom of Echis ocellatus: Neutralization of toxic activities and antivenomics.
Toxicon. 2016 Sep 01; 119:280-8.T

Abstract

Snakebite envenoming has a heavy burden in the public health in sub-Saharan Africa. The viperid species Echis ocellatus (carpet viper or saw-scaled viper) is the medically most important snake in the savannahs of western sub-Saharan Africa. Several antivenoms are being distributed and used in this region for the treatment of envenomings by E. ocellatus, but the preclinical efficacy of some of these antivenoms has not been assessed. The present study evaluated the preclinical efficacy against E. ocellatus venom of three polyspecific antivenoms: (a) Snake Venom Antiserum (Pan Africa), manufactured by Premium Serums and Vaccines (India); (b) Snake Venom Antiserum (Africa), manufactured by VINS Bioproducts (India); and (c) Antivipmyn(®) Africa, manufactured by Instituto Bioclon (Mexico). Antivenomics analysis revealed the ability of the three antivenoms to immunocapture the majority of components of the venoms of E. ocellatus from Cameroon, Nigeria and Mali, although their maximal immunocapturing capability varied. Bioclon and Premium Serums antivenoms were effective in the neutralization of lethal, hemorrhagic and in vitro coagulant activities of the venom of E. ocellatus from Cameroon, albeit with different potencies. VINS antivenom neutralized hemorrhagic activity of this venom, but failed to neutralize lethality at the highest antivenom dose tested, and had a low neutralizing efficacy against in vitro coagulant effect.

Authors+Show Affiliations

Laboratorio de Venómica Estructural y Funcional, Instituto de Biomedicina de Valencia, CSIC, Valencia, Spain. Electronic address: jcalvete@ibv.csic.es.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Laboratorio de Venómica Estructural y Funcional, Instituto de Biomedicina de Valencia, CSIC, Valencia, Spain.Laboratorio de Venómica Estructural y Funcional, Instituto de Biomedicina de Valencia, CSIC, Valencia, Spain.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Institut de Recherche pour le Développement, UMR MERIT "Mother and Child Facing Tropical Diseases", Cotonou, Benin; Université Paris Descartes, Sorbonne Paris Cité, Faculté de Pharmacie, Avenue de l'Observatoire, Paris, France.Laboratorio de Venómica Estructural y Funcional, Instituto de Biomedicina de Valencia, CSIC, Valencia, Spain. Electronic address: dpla@ibv.csic.es.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica. Electronic address: jose.gutierrez@ucr.ac.cr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27377229

Citation

Calvete, Juan J., et al. "Preclinical Evaluation of Three Polyspecific Antivenoms Against the Venom of Echis Ocellatus: Neutralization of Toxic Activities and Antivenomics." Toxicon : Official Journal of the International Society On Toxinology, vol. 119, 2016, pp. 280-8.
Calvete JJ, Arias AS, Rodríguez Y, et al. Preclinical evaluation of three polyspecific antivenoms against the venom of Echis ocellatus: Neutralization of toxic activities and antivenomics. Toxicon. 2016;119:280-8.
Calvete, J. J., Arias, A. S., Rodríguez, Y., Quesada-Bernat, S., Sánchez, L. V., Chippaux, J. P., Pla, D., & Gutiérrez, J. M. (2016). Preclinical evaluation of three polyspecific antivenoms against the venom of Echis ocellatus: Neutralization of toxic activities and antivenomics. Toxicon : Official Journal of the International Society On Toxinology, 119, 280-8. https://doi.org/10.1016/j.toxicon.2016.06.022
Calvete JJ, et al. Preclinical Evaluation of Three Polyspecific Antivenoms Against the Venom of Echis Ocellatus: Neutralization of Toxic Activities and Antivenomics. Toxicon. 2016 Sep 1;119:280-8. PubMed PMID: 27377229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preclinical evaluation of three polyspecific antivenoms against the venom of Echis ocellatus: Neutralization of toxic activities and antivenomics. AU - Calvete,Juan J, AU - Arias,Ana Silvia, AU - Rodríguez,Yania, AU - Quesada-Bernat,Sarai, AU - Sánchez,Laura V, AU - Chippaux,Jean Philippe, AU - Pla,Davinia, AU - Gutiérrez,José María, Y1 - 2016/07/01/ PY - 2016/05/10/received PY - 2016/06/23/revised PY - 2016/06/30/accepted PY - 2016/7/6/entrez PY - 2016/7/6/pubmed PY - 2017/6/24/medline KW - Africa KW - Antivenomics KW - Antivenoms KW - Echis ocellatus venom KW - Lethality KW - Neutralization SP - 280 EP - 8 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 119 N2 - Snakebite envenoming has a heavy burden in the public health in sub-Saharan Africa. The viperid species Echis ocellatus (carpet viper or saw-scaled viper) is the medically most important snake in the savannahs of western sub-Saharan Africa. Several antivenoms are being distributed and used in this region for the treatment of envenomings by E. ocellatus, but the preclinical efficacy of some of these antivenoms has not been assessed. The present study evaluated the preclinical efficacy against E. ocellatus venom of three polyspecific antivenoms: (a) Snake Venom Antiserum (Pan Africa), manufactured by Premium Serums and Vaccines (India); (b) Snake Venom Antiserum (Africa), manufactured by VINS Bioproducts (India); and (c) Antivipmyn(®) Africa, manufactured by Instituto Bioclon (Mexico). Antivenomics analysis revealed the ability of the three antivenoms to immunocapture the majority of components of the venoms of E. ocellatus from Cameroon, Nigeria and Mali, although their maximal immunocapturing capability varied. Bioclon and Premium Serums antivenoms were effective in the neutralization of lethal, hemorrhagic and in vitro coagulant activities of the venom of E. ocellatus from Cameroon, albeit with different potencies. VINS antivenom neutralized hemorrhagic activity of this venom, but failed to neutralize lethality at the highest antivenom dose tested, and had a low neutralizing efficacy against in vitro coagulant effect. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/27377229/Preclinical_evaluation_of_three_polyspecific_antivenoms_against_the_venom_of_Echis_ocellatus:_Neutralization_of_toxic_activities_and_antivenomics_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(16)30176-3 DB - PRIME DP - Unbound Medicine ER -