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Phytochemical and biological studies on Alnus glutinosa subsp. glutinosa, A. orientalis var. orientalis and A. orientalis var. pubescens leaves.
J Ethnopharmacol. 2016 Nov 04; 192:148-160.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Alnus species have been used for the treatment of rheumatism, hemorrhoids and for wound healing in folk medicine.

MATERIALS AND METHODS

Aqueous and methanol extracts of A. glutinosa (L.) Gaertner subsp. glutinosa, A. orientalis Decne. var. orientalis and A. orientalis var. pubescens Dippel leaves were evaluated for their wound healing, anti-inflammatory and antioxidant activities. In vivo wound models of linear incision and circular excision were performed. "Inhibition of acetic acid-induced capillary permeability", "carrageenan-induced hind paw edema" and T"PA-induced ear edema" assays were applied to determine the anti-inflammatory effects. For the antioxidant activity assessment, DPPH and ABTS radicals scavenging effect, reducing power and denaturation of nonspecific hydroxyl radical-targeted 2-deoxyribose were used. In vitro inhibitory effects on enzymes hyaluronidase, collagenase and elastase were evaluated. The methanol extract of the leaves of A. glutinosa subsp. glutinosa (MB), the most potent extract, was fractionated by bioassay-guided fractionation technique. The structure of the isolated compound was determined as shikimic acid by using NMR and IR analyses.

RESULTS

MB increased the wound tension by 42.79% value and provided a contraction by 51.58%. Wound tension, contraction capacity and tissue hydroxyproline levels were increased with the application of the fraction EtOAc: MeOH (Fr. D), subfraction D27-38 and shikimic acid. In the "inhibition of acetic acid-induced capillary permeability" assay, MB, Fr. D, subfraction D27-38 and shikimic acid inhibited the permeability with significant inhibition values of 30.22%, 32.46%, 38.24% and 27.19%, respectively. In carrageenan-induced hind paw edema model, MB displayed 29.1% inhibition. Likewise, Fr. D, subfraction D27-38, and shikimic acid were found to exhibit remarkable anti-inflammatory and antioxidant effects. Shikimic acid exhibited significant inhibitory effect (38.24%) on the hyaluronidase enzyme.

CONCLUSIONS

This is the first and unique study that investigates wound healing, anti-inflammatory and antioxidant effects of some Alnus taxons growing in Turkey. According to the results, shikimic acid was found to be the major compound responsible from the activity.

Authors+Show Affiliations

Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey; Turkish Medicines and Medical Devices Agency, 06520 Ankara, Turkey.Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, 06330 Ankara, Turkey.Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200 Afyonkarahisar, Turkey.Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, 06330 Ankara, Turkey. Electronic address: esrak@gazi.edu.tr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27381042

Citation

Altınyay, Çiğdem, et al. "Phytochemical and Biological Studies On Alnus Glutinosa Subsp. Glutinosa, A. Orientalis Var. Orientalis and A. Orientalis Var. Pubescens Leaves." Journal of Ethnopharmacology, vol. 192, 2016, pp. 148-160.
Altınyay Ç, Süntar I, Altun L, et al. Phytochemical and biological studies on Alnus glutinosa subsp. glutinosa, A. orientalis var. orientalis and A. orientalis var. pubescens leaves. J Ethnopharmacol. 2016;192:148-160.
Altınyay, Ç., Süntar, I., Altun, L., Keleş, H., & Küpeli Akkol, E. (2016). Phytochemical and biological studies on Alnus glutinosa subsp. glutinosa, A. orientalis var. orientalis and A. orientalis var. pubescens leaves. Journal of Ethnopharmacology, 192, 148-160. https://doi.org/10.1016/j.jep.2016.07.007
Altınyay Ç, et al. Phytochemical and Biological Studies On Alnus Glutinosa Subsp. Glutinosa, A. Orientalis Var. Orientalis and A. Orientalis Var. Pubescens Leaves. J Ethnopharmacol. 2016 Nov 4;192:148-160. PubMed PMID: 27381042.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytochemical and biological studies on Alnus glutinosa subsp. glutinosa, A. orientalis var. orientalis and A. orientalis var. pubescens leaves. AU - Altınyay,Çiğdem, AU - Süntar,Ipek, AU - Altun,Levent, AU - Keleş,Hikmet, AU - Küpeli Akkol,Esra, Y1 - 2016/07/02/ PY - 2016/02/06/received PY - 2016/06/24/revised PY - 2016/07/01/accepted PY - 2016/7/7/pubmed PY - 2017/4/22/medline PY - 2016/7/7/entrez KW - Alnus KW - Anti-inflammatory KW - Antioxidant KW - Betulaceae KW - Isolation KW - Wound healing SP - 148 EP - 160 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 192 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Alnus species have been used for the treatment of rheumatism, hemorrhoids and for wound healing in folk medicine. MATERIALS AND METHODS: Aqueous and methanol extracts of A. glutinosa (L.) Gaertner subsp. glutinosa, A. orientalis Decne. var. orientalis and A. orientalis var. pubescens Dippel leaves were evaluated for their wound healing, anti-inflammatory and antioxidant activities. In vivo wound models of linear incision and circular excision were performed. "Inhibition of acetic acid-induced capillary permeability", "carrageenan-induced hind paw edema" and T"PA-induced ear edema" assays were applied to determine the anti-inflammatory effects. For the antioxidant activity assessment, DPPH and ABTS radicals scavenging effect, reducing power and denaturation of nonspecific hydroxyl radical-targeted 2-deoxyribose were used. In vitro inhibitory effects on enzymes hyaluronidase, collagenase and elastase were evaluated. The methanol extract of the leaves of A. glutinosa subsp. glutinosa (MB), the most potent extract, was fractionated by bioassay-guided fractionation technique. The structure of the isolated compound was determined as shikimic acid by using NMR and IR analyses. RESULTS: MB increased the wound tension by 42.79% value and provided a contraction by 51.58%. Wound tension, contraction capacity and tissue hydroxyproline levels were increased with the application of the fraction EtOAc: MeOH (Fr. D), subfraction D27-38 and shikimic acid. In the "inhibition of acetic acid-induced capillary permeability" assay, MB, Fr. D, subfraction D27-38 and shikimic acid inhibited the permeability with significant inhibition values of 30.22%, 32.46%, 38.24% and 27.19%, respectively. In carrageenan-induced hind paw edema model, MB displayed 29.1% inhibition. Likewise, Fr. D, subfraction D27-38, and shikimic acid were found to exhibit remarkable anti-inflammatory and antioxidant effects. Shikimic acid exhibited significant inhibitory effect (38.24%) on the hyaluronidase enzyme. CONCLUSIONS: This is the first and unique study that investigates wound healing, anti-inflammatory and antioxidant effects of some Alnus taxons growing in Turkey. According to the results, shikimic acid was found to be the major compound responsible from the activity. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/27381042/Phytochemical_and_biological_studies_on_Alnus_glutinosa_subsp__glutinosa_A__orientalis_var__orientalis_and_A__orientalis_var__pubescens_leaves_ DB - PRIME DP - Unbound Medicine ER -