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Molecular basis of the remarkable species selectivity of an insecticidal sodium channel toxin from the African spider Augacephalus ezendami.
Sci Rep. 2016 07 07; 6:29538.SR

Abstract

The inexorable decline in the armament of registered chemical insecticides has stimulated research into environmentally-friendly alternatives. Insecticidal spider-venom peptides are promising candidates for bioinsecticide development but it is challenging to find peptides that are specific for targeted pests. In the present study, we isolated an insecticidal peptide (Ae1a) from venom of the African spider Augacephalus ezendami (family Theraphosidae). Injection of Ae1a into sheep blowflies (Lucilia cuprina) induced rapid but reversible paralysis. In striking contrast, Ae1a was lethal to closely related fruit flies (Drosophila melanogaster) but induced no adverse effects in the recalcitrant lepidopteran pest Helicoverpa armigera. Electrophysiological experiments revealed that Ae1a potently inhibits the voltage-gated sodium channel BgNaV1 from the German cockroach Blattella germanica by shifting the threshold for channel activation to more depolarized potentials. In contrast, Ae1a failed to significantly affect sodium currents in dorsal unpaired median neurons from the American cockroach Periplaneta americana. We show that Ae1a interacts with the domain II voltage sensor and that sensitivity to the toxin is conferred by natural sequence variations in the S1-S2 loop of domain II. The phyletic specificity of Ae1a provides crucial information for development of sodium channel insecticides that target key insect pests without harming beneficial species.

Authors+Show Affiliations

Institute for Molecular Bioscience, The University of Queensland, St. Lucia, QLD 4072, Australia.Institute for Molecular Bioscience, The University of Queensland, St. Lucia, QLD 4072, Australia.Institute for Molecular Bioscience, The University of Queensland, St. Lucia, QLD 4072, Australia.School of Medical &Molecular Biosciences, University of Technology, Sydney, NSW 2007, Australia.Department of Physiology &Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.Institute of Ecology &Evolution, University of Bern, CH 3012 Bern, Switzerland.FIOCRUZ/Centro de Pesquisas René Rachou, Belo Horizonte, CEP 30190-002, MG, Brazil.FIOCRUZ/Centro de Pesquisas René Rachou, Belo Horizonte, CEP 30190-002, MG, Brazil.School of Medical &Molecular Biosciences, University of Technology, Sydney, NSW 2007, Australia.Department of Physiology &Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.Institute for Molecular Bioscience, The University of Queensland, St. Lucia, QLD 4072, Australia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27383378

Citation

Herzig, Volker, et al. "Molecular Basis of the Remarkable Species Selectivity of an Insecticidal Sodium Channel Toxin From the African Spider Augacephalus Ezendami." Scientific Reports, vol. 6, 2016, p. 29538.
Herzig V, Ikonomopoulou M, Smith JJ, et al. Molecular basis of the remarkable species selectivity of an insecticidal sodium channel toxin from the African spider Augacephalus ezendami. Sci Rep. 2016;6:29538.
Herzig, V., Ikonomopoulou, M., Smith, J. J., Dziemborowicz, S., Gilchrist, J., Kuhn-Nentwig, L., Rezende, F. O., Moreira, L. A., Nicholson, G. M., Bosmans, F., & King, G. F. (2016). Molecular basis of the remarkable species selectivity of an insecticidal sodium channel toxin from the African spider Augacephalus ezendami. Scientific Reports, 6, 29538. https://doi.org/10.1038/srep29538
Herzig V, et al. Molecular Basis of the Remarkable Species Selectivity of an Insecticidal Sodium Channel Toxin From the African Spider Augacephalus Ezendami. Sci Rep. 2016 07 7;6:29538. PubMed PMID: 27383378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular basis of the remarkable species selectivity of an insecticidal sodium channel toxin from the African spider Augacephalus ezendami. AU - Herzig,Volker, AU - Ikonomopoulou,Maria, AU - Smith,Jennifer J, AU - Dziemborowicz,Sławomir, AU - Gilchrist,John, AU - Kuhn-Nentwig,Lucia, AU - Rezende,Fernanda Oliveira, AU - Moreira,Luciano Andrade, AU - Nicholson,Graham M, AU - Bosmans,Frank, AU - King,Glenn F, Y1 - 2016/07/07/ PY - 2016/02/03/received PY - 2016/06/20/accepted PY - 2016/7/8/entrez PY - 2016/7/8/pubmed PY - 2018/6/21/medline SP - 29538 EP - 29538 JF - Scientific reports JO - Sci Rep VL - 6 N2 - The inexorable decline in the armament of registered chemical insecticides has stimulated research into environmentally-friendly alternatives. Insecticidal spider-venom peptides are promising candidates for bioinsecticide development but it is challenging to find peptides that are specific for targeted pests. In the present study, we isolated an insecticidal peptide (Ae1a) from venom of the African spider Augacephalus ezendami (family Theraphosidae). Injection of Ae1a into sheep blowflies (Lucilia cuprina) induced rapid but reversible paralysis. In striking contrast, Ae1a was lethal to closely related fruit flies (Drosophila melanogaster) but induced no adverse effects in the recalcitrant lepidopteran pest Helicoverpa armigera. Electrophysiological experiments revealed that Ae1a potently inhibits the voltage-gated sodium channel BgNaV1 from the German cockroach Blattella germanica by shifting the threshold for channel activation to more depolarized potentials. In contrast, Ae1a failed to significantly affect sodium currents in dorsal unpaired median neurons from the American cockroach Periplaneta americana. We show that Ae1a interacts with the domain II voltage sensor and that sensitivity to the toxin is conferred by natural sequence variations in the S1-S2 loop of domain II. The phyletic specificity of Ae1a provides crucial information for development of sodium channel insecticides that target key insect pests without harming beneficial species. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/27383378/Molecular_basis_of_the_remarkable_species_selectivity_of_an_insecticidal_sodium_channel_toxin_from_the_African_spider_Augacephalus_ezendami_ L2 - https://doi.org/10.1038/srep29538 DB - PRIME DP - Unbound Medicine ER -