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Immunophenotypic Modulation of the Blast Cells in Childhood Acute Lymphoblastic Leukemia Minimal Residual Disease Detection.
Folia Med (Plovdiv). 2016 03 01; 58(1):28-35.FM

Abstract

Early clearance of leukemic cells during induction therapy of childhood acute lymphoblastic leukemia (ALL) is a basis for treatment optimization. Currently, the most widely used methods for the detection of minute residual malignant cells in the bone marrow and/or peripheral blood, minimal residual disease (MRD), are PCR and flow cytometry (FCM). Immunophenotypic modulation (IM) is a well known factor that can hamper the accurate FCM analysis.

AIM

To report the IM detected by 8-color FCM during the BFM-type remission induction in 24 consecutive MRD-positive samples of children with B-cell precursor ALL and the possible implications for MRD detection.

PATIENTS AND METHODS

Between 2010 and 2012 we prospectively followed up the MRD on days 15 and 33 of induction treatment in bone marrow (BM) samples and on day 8 in peripheral blood (PB). The IM was assessed by comparative analyses of the changes in the mean fluorescence intensity of 7 highly relevant antigens expressed by the leukemic cells and normal B-lymphocytes.

RESULTS

IM occurred, to different extents, in all analyzed day 15 BM and in most day 33 BM samples. Statistically significant changes in the MFI-levels of four CDs expressed by the leukemic blasts were observed: downmodulation of CD10, CD19 and CD34 and upmodulation of CD20. No changes in the expression of CD38, CD58 and CD45 were noticed.

CONCLUSIONS

Measuring the MRD by standardized 8-color flow cytometry helps improve the monitoring of the disease, leading to better therapeutic results. However, the IM of the different antigens expressed by the leukemic blasts should be taken into consideration and cautiously analyzed.

Authors+Show Affiliations

Department of Pediatrics and Medical Genetics, Medical University of Plovdiv, Plovdiv, BulgariaDepartment of Pediatrics and Medical Genetics, Medical University of Plovdiv, Plovdiv, BulgariaDepartment of Microbiology and Immunology, Medical University of Plovdiv, Plovdiv, BulgariaDepartment of Pediatrics and Medical Genetics, Medical University of Plovdiv, Plovdiv, BulgariaDepartment of Pediatrics and Medical Genetics, Medical University of Plovdiv, Plovdiv, BulgariaDepartment of Pediatrics and Medical Genetics, Medical University of Varna, Varna, BulgariaDepartment of Pediatrics and Medical Genetics, Medical University of Varna, Varna, BulgariaDepartment of Pediatrics and Medical Genetics, Medical University of Plovdiv, Plovdiv, Bulgaria

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27383875

Citation

Burnusuzov, Hasan A., et al. "Immunophenotypic Modulation of the Blast Cells in Childhood Acute Lymphoblastic Leukemia Minimal Residual Disease Detection." Folia Medica, vol. 58, no. 1, 2016, pp. 28-35.
Burnusuzov HA, Spasova MI, Murdjeva MA, et al. Immunophenotypic Modulation of the Blast Cells in Childhood Acute Lymphoblastic Leukemia Minimal Residual Disease Detection. Folia Med (Plovdiv). 2016;58(1):28-35.
Burnusuzov, H. A., Spasova, M. I., Murdjeva, M. A., Stoyanova, A. A., Mumdziev, I. N., Kaleva, V. I., Belcheva, M. I., & Bosheva, M. N. (2016). Immunophenotypic Modulation of the Blast Cells in Childhood Acute Lymphoblastic Leukemia Minimal Residual Disease Detection. Folia Medica, 58(1), 28-35. https://doi.org/10.1515/folmed-2016-0004
Burnusuzov HA, et al. Immunophenotypic Modulation of the Blast Cells in Childhood Acute Lymphoblastic Leukemia Minimal Residual Disease Detection. Folia Med (Plovdiv). 2016 03 1;58(1):28-35. PubMed PMID: 27383875.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunophenotypic Modulation of the Blast Cells in Childhood Acute Lymphoblastic Leukemia Minimal Residual Disease Detection. AU - Burnusuzov,Hasan A, AU - Spasova,Mariya I, AU - Murdjeva,Mariana A, AU - Stoyanova,Angelina A, AU - Mumdziev,Ivan N, AU - Kaleva,Valeriya I, AU - Belcheva,Milena I, AU - Bosheva,Miroslava N, PY - 2016/02/17/received PY - 2016/04/11/accepted PY - 2016/7/8/entrez PY - 2016/7/8/pubmed PY - 2017/1/4/medline SP - 28 EP - 35 JF - Folia medica JO - Folia Med (Plovdiv) VL - 58 IS - 1 N2 - UNLABELLED: Early clearance of leukemic cells during induction therapy of childhood acute lymphoblastic leukemia (ALL) is a basis for treatment optimization. Currently, the most widely used methods for the detection of minute residual malignant cells in the bone marrow and/or peripheral blood, minimal residual disease (MRD), are PCR and flow cytometry (FCM). Immunophenotypic modulation (IM) is a well known factor that can hamper the accurate FCM analysis. AIM: To report the IM detected by 8-color FCM during the BFM-type remission induction in 24 consecutive MRD-positive samples of children with B-cell precursor ALL and the possible implications for MRD detection. PATIENTS AND METHODS: Between 2010 and 2012 we prospectively followed up the MRD on days 15 and 33 of induction treatment in bone marrow (BM) samples and on day 8 in peripheral blood (PB). The IM was assessed by comparative analyses of the changes in the mean fluorescence intensity of 7 highly relevant antigens expressed by the leukemic cells and normal B-lymphocytes. RESULTS: IM occurred, to different extents, in all analyzed day 15 BM and in most day 33 BM samples. Statistically significant changes in the MFI-levels of four CDs expressed by the leukemic blasts were observed: downmodulation of CD10, CD19 and CD34 and upmodulation of CD20. No changes in the expression of CD38, CD58 and CD45 were noticed. CONCLUSIONS: Measuring the MRD by standardized 8-color flow cytometry helps improve the monitoring of the disease, leading to better therapeutic results. However, the IM of the different antigens expressed by the leukemic blasts should be taken into consideration and cautiously analyzed. SN - 0204-8043 UR - https://www.unboundmedicine.com/medline/citation/27383875/Immunophenotypic_Modulation_of_the_Blast_Cells_in_Childhood_Acute_Lymphoblastic_Leukemia_Minimal_Residual_Disease_Detection_ L2 - http://www.diseaseinfosearch.org/result/188 DB - PRIME DP - Unbound Medicine ER -