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Just add water: cannabinoid discrimination in a water T-maze with FAAH(-/-) and FAAH(+/+) mice.
Behav Pharmacol. 2016 08; 27(5):479-84.BP

Abstract

Incomplete overlap in the discriminative stimulus effects of Δ-tetrahydrocannabinol (THC) and the endocannabinoids, anandamide (AEA) and 2-arachidonoylglycerol has been reported in food-reinforced tasks. The aim of this study was to examine cannabinoid discriminative stimulus effects in a nonappetitive procedure. Adult male mice lacking the gene for AEA's major metabolic enzyme, fatty acid amide hydrolase (FAAH), and FAAH mice were trained to discriminate THC or AEA in a water T-maze, in which the response was swimming to an escape platform on the injection-appropriate side. JZL184, a monoacylglycerol lipase inhibitor, was also tested. FAAH mice showed faster acquisition than FAAH mice. THC and AEA fully substituted, with only minor cross-procedure potency variations. Incomplete substitution of JZL184 was observed in THC-trained FAAH mice in the water-maze task, as contrasted with full substitution in a food-reinforced nose-poke procedure. Stress-induced changes in AEA and/or 2-arachidonoylglycerol concentrations in the brain may have mediated this attenuation. JZL184 also partially substituted in AEA-trained FAAH mice in the water maze, suggesting incomplete overlap in the stimulus effects of AEA and JZL184. Through the use of a novel water-maze procedure, the present study supports the work of previous behavioral pharmacologists in showing the robustness of the discrimination paradigm.

Authors+Show Affiliations

aRTI International, Research Triangle Park, North CarolinabThe Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CaliforniacDepartment of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, Virginia, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27385208

Citation

Wiley, Jenny L., et al. "Just Add Water: Cannabinoid Discrimination in a Water T-maze With FAAH(-/-) and FAAH(+/+) Mice." Behavioural Pharmacology, vol. 27, no. 5, 2016, pp. 479-84.
Wiley JL, Lefever TW, Pulley NS, et al. Just add water: cannabinoid discrimination in a water T-maze with FAAH(-/-) and FAAH(+/+) mice. Behav Pharmacol. 2016;27(5):479-84.
Wiley, J. L., Lefever, T. W., Pulley, N. S., Marusich, J. A., Cravatt, B. F., & Lichtman, A. H. (2016). Just add water: cannabinoid discrimination in a water T-maze with FAAH(-/-) and FAAH(+/+) mice. Behavioural Pharmacology, 27(5), 479-84. https://doi.org/10.1097/FBP.0000000000000228
Wiley JL, et al. Just Add Water: Cannabinoid Discrimination in a Water T-maze With FAAH(-/-) and FAAH(+/+) Mice. Behav Pharmacol. 2016;27(5):479-84. PubMed PMID: 27385208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Just add water: cannabinoid discrimination in a water T-maze with FAAH(-/-) and FAAH(+/+) mice. AU - Wiley,Jenny L, AU - Lefever,Timothy W, AU - Pulley,Nikita S, AU - Marusich,Julie A, AU - Cravatt,Benjamin F, AU - Lichtman,Aron H, PY - 2016/7/8/entrez PY - 2016/7/8/pubmed PY - 2017/11/9/medline SP - 479 EP - 84 JF - Behavioural pharmacology JO - Behav Pharmacol VL - 27 IS - 5 N2 - Incomplete overlap in the discriminative stimulus effects of Δ-tetrahydrocannabinol (THC) and the endocannabinoids, anandamide (AEA) and 2-arachidonoylglycerol has been reported in food-reinforced tasks. The aim of this study was to examine cannabinoid discriminative stimulus effects in a nonappetitive procedure. Adult male mice lacking the gene for AEA's major metabolic enzyme, fatty acid amide hydrolase (FAAH), and FAAH mice were trained to discriminate THC or AEA in a water T-maze, in which the response was swimming to an escape platform on the injection-appropriate side. JZL184, a monoacylglycerol lipase inhibitor, was also tested. FAAH mice showed faster acquisition than FAAH mice. THC and AEA fully substituted, with only minor cross-procedure potency variations. Incomplete substitution of JZL184 was observed in THC-trained FAAH mice in the water-maze task, as contrasted with full substitution in a food-reinforced nose-poke procedure. Stress-induced changes in AEA and/or 2-arachidonoylglycerol concentrations in the brain may have mediated this attenuation. JZL184 also partially substituted in AEA-trained FAAH mice in the water maze, suggesting incomplete overlap in the stimulus effects of AEA and JZL184. Through the use of a novel water-maze procedure, the present study supports the work of previous behavioral pharmacologists in showing the robustness of the discrimination paradigm. SN - 1473-5849 UR - https://www.unboundmedicine.com/medline/citation/27385208/Just_add_water:_cannabinoid_discrimination_in_a_water_T_maze_with_FAAH__/___and_FAAH_+/+__mice_ L2 - https://doi.org/10.1097/FBP.0000000000000228 DB - PRIME DP - Unbound Medicine ER -