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Intraspecific Polymorphisms of Cytogenetic Markers Mapped on Chromosomes of Triticum polonicum L.
PLoS One. 2016; 11(7):e0158883.Plos

Abstract

Triticum genus encloses several tetraploid species that are used as genetic stocks for expanding the genetic variability of wheat (Triticum aestivum L.). Although the T. aestivum (2n = 6x = 42, AABBDD) and T. durum (2n = 4x = 28, AABB) karyotypes were well examined by chromosome staining, Giemsa C-banding and FISH markers, other tetraploids are still poorly characterized. Here, we established and compared the fluorescence in situ hybridization (FISH) patterns on chromosomes of 20 accessions of T. polonicum species using different repetitive sequences from BAC library of wheat 'Chinese Spring'. The chromosome patterns of Polish wheat were compared to tetraploid (2n = 4x = 28, AABB) Triticum species: T. durum, T. diccocon and T. turanicum, as well. A combination of pTa-86, pTa-535 and pTa-713 probes was the most informative among 6 DNA probes tested. Probe pTa-k374, which is similar to 28S rDNA sequence enabled to distinguish signal size and location differences, as well as rDNA loci elimination. Furthermore, pTa-465 and pTa-k566 probes are helpful for the detection of similar organized chromosomes. The polymorphisms of signals distribution were observed in 2A, 2B, 3B, 5B, 6A and 7B chromosomes. Telomeric region of the short arm of 6B chromosome was the most polymorphic. Our work is novel and contributes to the understanding of T. polonicum genome organization which is essential to develop successful advanced breeding strategies for wheat. Collection and characterization of this germplasm can contribute to the wheat biodiversity safeguard.

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Authors+Show Affiliations

Kwiatek M
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.
Majka M
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.
Majka J
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.
Belter J
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.
Suchowilska E
Department of Plant Breeding and Seed Production, University of Warmia and Mazury in Olsztyn, Pl. Łódzki 3, 10-727 Olsztyn, Poland.
Wachowska U
Department of Entomology, Phytopathology and Molecular Diagnostics, University of Warmia and Mazury in Olsztyn, Pl. Łódzki 3, 10-727 Olsztyn, Poland.
Wiwart M
Department of Plant Breeding and Seed Production, University of Warmia and Mazury in Olsztyn, Pl. Łódzki 3, 10-727 Olsztyn, Poland.
Wiśniewska H
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.

MeSH

Chromosomes, PlantCytogeneticsDNA, PlantDNA, RibosomalGenetic MarkersPolymorphism, GeneticRNA, PlantRNA, Ribosomal, 28STriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27391447

Citation

Kwiatek, Michał, et al. "Intraspecific Polymorphisms of Cytogenetic Markers Mapped On Chromosomes of Triticum Polonicum L." PloS One, vol. 11, no. 7, 2016, pp. e0158883.
Kwiatek M, Majka M, Majka J, et al. Intraspecific Polymorphisms of Cytogenetic Markers Mapped on Chromosomes of Triticum polonicum L. PLoS One. 2016;11(7):e0158883.
Kwiatek, M., Majka, M., Majka, J., Belter, J., Suchowilska, E., Wachowska, U., Wiwart, M., & Wiśniewska, H. (2016). Intraspecific Polymorphisms of Cytogenetic Markers Mapped on Chromosomes of Triticum polonicum L. PloS One, 11(7), e0158883. https://doi.org/10.1371/journal.pone.0158883
Kwiatek M, et al. Intraspecific Polymorphisms of Cytogenetic Markers Mapped On Chromosomes of Triticum Polonicum L. PLoS One. 2016;11(7):e0158883. PubMed PMID: 27391447.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intraspecific Polymorphisms of Cytogenetic Markers Mapped on Chromosomes of Triticum polonicum L. AU - Kwiatek,Michał, AU - Majka,Maciej, AU - Majka,Joanna, AU - Belter,Jolanta, AU - Suchowilska,Elżbieta, AU - Wachowska,Urszula, AU - Wiwart,Marian, AU - Wiśniewska,Halina, Y1 - 2016/07/08/ PY - 2016/04/22/received PY - 2016/06/23/accepted PY - 2016/7/9/entrez PY - 2016/7/9/pubmed PY - 2017/7/21/medline SP - e0158883 EP - e0158883 JF - PloS one JO - PLoS One VL - 11 IS - 7 N2 - Triticum genus encloses several tetraploid species that are used as genetic stocks for expanding the genetic variability of wheat (Triticum aestivum L.). Although the T. aestivum (2n = 6x = 42, AABBDD) and T. durum (2n = 4x = 28, AABB) karyotypes were well examined by chromosome staining, Giemsa C-banding and FISH markers, other tetraploids are still poorly characterized. Here, we established and compared the fluorescence in situ hybridization (FISH) patterns on chromosomes of 20 accessions of T. polonicum species using different repetitive sequences from BAC library of wheat 'Chinese Spring'. The chromosome patterns of Polish wheat were compared to tetraploid (2n = 4x = 28, AABB) Triticum species: T. durum, T. diccocon and T. turanicum, as well. A combination of pTa-86, pTa-535 and pTa-713 probes was the most informative among 6 DNA probes tested. Probe pTa-k374, which is similar to 28S rDNA sequence enabled to distinguish signal size and location differences, as well as rDNA loci elimination. Furthermore, pTa-465 and pTa-k566 probes are helpful for the detection of similar organized chromosomes. The polymorphisms of signals distribution were observed in 2A, 2B, 3B, 5B, 6A and 7B chromosomes. Telomeric region of the short arm of 6B chromosome was the most polymorphic. Our work is novel and contributes to the understanding of T. polonicum genome organization which is essential to develop successful advanced breeding strategies for wheat. Collection and characterization of this germplasm can contribute to the wheat biodiversity safeguard. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/27391447/Intraspecific_Polymorphisms_of_Cytogenetic_Markers_Mapped_on_Chromosomes_of_Triticum_polonicum_L_ DB - PRIME DP - Unbound Medicine ER -