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Antioxidant activities and oxidative stress inhibitory effects of ethanol extracts from Cornus officinalis on raw 264.7 cells.
BMC Complement Altern Med. 2016 Jul 08; 16:196.BC

Abstract

BACKGROUND

Cornus officinalis, is a deciduous tree native to the eastern Asia, distributes mainly in (e.g. Korea, as well as China, and Japan). It is used as folk medicine to backache, polyuria, hypertension and nervous breakdown. Pharmacological studies have demonstrated that C. officinalis possess anti-oxidant, anti-hyperglycemic, and immune regulatory effects. However, reports on the antioxidant activity of C. officinalis have been limited to in vitro radical scavenging studies. Its mechanism of action within the cell at the genetic level especially has not yet been clearly defined. Therefore, we investigated the anti-antioxidant activities of C. officinalis in RAW 264.7 cells.

METHODS

The antioxidant activities and protective effects of C. officinalis ethanol extract on cell damage and the antioxidant enzyme system in lipopolysaccharide (LPS)-induced oxidative stress-damaged RAW 264.7 cells were assessed. To measure the effects of C. officinalis on antioxidant activities, we used the following methods: Total phenol and flavonoid contents, DPPH scavenging activity assay, ABTS scavenging activity assay, FRAP value measurement, xanthine oxidase activity assay, ROS generation measurement and real time PCR.

RESULTS

The total phenol and flavonoid contents of C. officinalis extracts were 27.04 mg GAE/g and 3.70 mg QE/g, respectively. The antioxidant activities of C. officinalis extracts increased in a dose-dependent manner: the IC50 values for DPPH and ABTS radical scavenging activities of C. officinalis extracts were 99.32 μg/mL and 138.51 μg/mL, respectively. C. officinalis extracts inhibited xanthine oxidase activity and reactive oxygen species generation. The expression of antioxidant enzymes, Cu/ZnSOD, MnSOD, catalase, and glutathione peroxidase increased upon treatment with C. officinalis extracts at 100 μg/mL, compared to that in the LPS-treated group.

CONCLUSIONS

These results suggest the therapeutic potential of C. officinalis extract as an anti-oxidant agent.

Authors+Show Affiliations

Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea. kah366@korea.kr.Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea.Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27391600

Citation

Hwang, Kyung-A, et al. "Antioxidant Activities and Oxidative Stress Inhibitory Effects of Ethanol Extracts From Cornus Officinalis On Raw 264.7 Cells." BMC Complementary and Alternative Medicine, vol. 16, 2016, p. 196.
Hwang KA, Hwang YJ, Song J. Antioxidant activities and oxidative stress inhibitory effects of ethanol extracts from Cornus officinalis on raw 264.7 cells. BMC Complement Altern Med. 2016;16:196.
Hwang, K. A., Hwang, Y. J., & Song, J. (2016). Antioxidant activities and oxidative stress inhibitory effects of ethanol extracts from Cornus officinalis on raw 264.7 cells. BMC Complementary and Alternative Medicine, 16, 196. https://doi.org/10.1186/s12906-016-1172-3
Hwang KA, Hwang YJ, Song J. Antioxidant Activities and Oxidative Stress Inhibitory Effects of Ethanol Extracts From Cornus Officinalis On Raw 264.7 Cells. BMC Complement Altern Med. 2016 Jul 8;16:196. PubMed PMID: 27391600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activities and oxidative stress inhibitory effects of ethanol extracts from Cornus officinalis on raw 264.7 cells. AU - Hwang,Kyung-A, AU - Hwang,Yu-Jin, AU - Song,Jin, Y1 - 2016/07/08/ PY - 2016/01/07/received PY - 2016/06/14/accepted PY - 2016/7/9/entrez PY - 2016/7/9/pubmed PY - 2017/2/12/medline KW - Antioxidant activity KW - Cornus officinalis KW - Oxidative stress SP - 196 EP - 196 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 16 N2 - BACKGROUND: Cornus officinalis, is a deciduous tree native to the eastern Asia, distributes mainly in (e.g. Korea, as well as China, and Japan). It is used as folk medicine to backache, polyuria, hypertension and nervous breakdown. Pharmacological studies have demonstrated that C. officinalis possess anti-oxidant, anti-hyperglycemic, and immune regulatory effects. However, reports on the antioxidant activity of C. officinalis have been limited to in vitro radical scavenging studies. Its mechanism of action within the cell at the genetic level especially has not yet been clearly defined. Therefore, we investigated the anti-antioxidant activities of C. officinalis in RAW 264.7 cells. METHODS: The antioxidant activities and protective effects of C. officinalis ethanol extract on cell damage and the antioxidant enzyme system in lipopolysaccharide (LPS)-induced oxidative stress-damaged RAW 264.7 cells were assessed. To measure the effects of C. officinalis on antioxidant activities, we used the following methods: Total phenol and flavonoid contents, DPPH scavenging activity assay, ABTS scavenging activity assay, FRAP value measurement, xanthine oxidase activity assay, ROS generation measurement and real time PCR. RESULTS: The total phenol and flavonoid contents of C. officinalis extracts were 27.04 mg GAE/g and 3.70 mg QE/g, respectively. The antioxidant activities of C. officinalis extracts increased in a dose-dependent manner: the IC50 values for DPPH and ABTS radical scavenging activities of C. officinalis extracts were 99.32 μg/mL and 138.51 μg/mL, respectively. C. officinalis extracts inhibited xanthine oxidase activity and reactive oxygen species generation. The expression of antioxidant enzymes, Cu/ZnSOD, MnSOD, catalase, and glutathione peroxidase increased upon treatment with C. officinalis extracts at 100 μg/mL, compared to that in the LPS-treated group. CONCLUSIONS: These results suggest the therapeutic potential of C. officinalis extract as an anti-oxidant agent. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/27391600/Antioxidant_activities_and_oxidative_stress_inhibitory_effects_of_ethanol_extracts_from_Cornus_officinalis_on_raw_264_7_cells_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-016-1172-3 DB - PRIME DP - Unbound Medicine ER -