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Potential Application of the Oryza sativa Monodehydroascorbate Reductase Gene (OsMDHAR) to Improve the Stress Tolerance and Fermentative Capacity of Saccharomyces cerevisiae.
PLoS One. 2016; 11(7):e0158841.Plos

Abstract

Monodehydroascorbate reductase (MDHAR; EC 1.6.5.4) is an important enzyme for ascorbate recycling. To examine whether heterologous expression of MDHAR from Oryza sativa (OsMDHAR) can prevent the deleterious effects of unfavorable growth conditions, we constructed a transgenic yeast strain harboring a recombinant plasmid carrying OsMDHAR (p426GPD::OsMDHAR). OsMDHAR-expressing yeast cells displayed enhanced tolerance to hydrogen peroxide by maintaining redox homoeostasis, proteostasis, and the ascorbate (AsA)-like pool following the accumulation of antioxidant enzymes and molecules, metabolic enzymes, and molecular chaperones and their cofactors, compared to wild-type (WT) cells carrying vector alone. The addition of exogenous AsA or its analogue isoascorbic acid increased the viability of WT and ara2Δ cells under oxidative stress. Furthermore, the survival of OsMDHAR-expressing cells was greater than that of WT cells when cells at mid-log growth phase were exposed to high concentrations of ethanol. High OsMDHAR expression also improved the fermentative capacity of the yeast during glucose-based batch fermentation at a standard cultivation temperature (30°C). The alcohol yield of OsMDHAR-expressing transgenic yeast during fermentation was approximately 25% (0.18 g·g-1) higher than that of WT yeast. Accordingly, OsMDHAR-expressing transgenic yeast showed prolonged survival during the environmental stresses produced during fermentation. These results suggest that heterologous OsMDHAR expression increases tolerance to reactive oxygen species-induced oxidative stress by improving cellular redox homeostasis and improves survival during fermentation, which enhances fermentative capacity.

Authors+Show Affiliations

School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, Republic of Korea.Division of Biological Sciences, University of California San Diego, La Jolla, California, United States of America.Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration, Pyeongchang, Republic of Korea.Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, Republic of Korea.Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, Republic of Korea.Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, Republic of Korea.School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27392090

Citation

Kim, Il-Sup, et al. "Potential Application of the Oryza Sativa Monodehydroascorbate Reductase Gene (OsMDHAR) to Improve the Stress Tolerance and Fermentative Capacity of Saccharomyces Cerevisiae." PloS One, vol. 11, no. 7, 2016, pp. e0158841.
Kim IS, Kim YS, Kim YH, et al. Potential Application of the Oryza sativa Monodehydroascorbate Reductase Gene (OsMDHAR) to Improve the Stress Tolerance and Fermentative Capacity of Saccharomyces cerevisiae. PLoS One. 2016;11(7):e0158841.
Kim, I. S., Kim, Y. S., Kim, Y. H., Park, A. K., Kim, H. W., Lee, J. H., & Yoon, H. S. (2016). Potential Application of the Oryza sativa Monodehydroascorbate Reductase Gene (OsMDHAR) to Improve the Stress Tolerance and Fermentative Capacity of Saccharomyces cerevisiae. PloS One, 11(7), e0158841. https://doi.org/10.1371/journal.pone.0158841
Kim IS, et al. Potential Application of the Oryza Sativa Monodehydroascorbate Reductase Gene (OsMDHAR) to Improve the Stress Tolerance and Fermentative Capacity of Saccharomyces Cerevisiae. PLoS One. 2016;11(7):e0158841. PubMed PMID: 27392090.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potential Application of the Oryza sativa Monodehydroascorbate Reductase Gene (OsMDHAR) to Improve the Stress Tolerance and Fermentative Capacity of Saccharomyces cerevisiae. AU - Kim,Il-Sup, AU - Kim,Young-Saeng, AU - Kim,Yul-Ho, AU - Park,Ae-Kyung, AU - Kim,Han-Woo, AU - Lee,Jun-Hyuk, AU - Yoon,Ho-Sung, Y1 - 2016/07/08/ PY - 2016/04/22/received PY - 2016/06/22/accepted PY - 2016/7/9/entrez PY - 2016/7/9/pubmed PY - 2017/7/21/medline SP - e0158841 EP - e0158841 JF - PloS one JO - PLoS One VL - 11 IS - 7 N2 - Monodehydroascorbate reductase (MDHAR; EC 1.6.5.4) is an important enzyme for ascorbate recycling. To examine whether heterologous expression of MDHAR from Oryza sativa (OsMDHAR) can prevent the deleterious effects of unfavorable growth conditions, we constructed a transgenic yeast strain harboring a recombinant plasmid carrying OsMDHAR (p426GPD::OsMDHAR). OsMDHAR-expressing yeast cells displayed enhanced tolerance to hydrogen peroxide by maintaining redox homoeostasis, proteostasis, and the ascorbate (AsA)-like pool following the accumulation of antioxidant enzymes and molecules, metabolic enzymes, and molecular chaperones and their cofactors, compared to wild-type (WT) cells carrying vector alone. The addition of exogenous AsA or its analogue isoascorbic acid increased the viability of WT and ara2Δ cells under oxidative stress. Furthermore, the survival of OsMDHAR-expressing cells was greater than that of WT cells when cells at mid-log growth phase were exposed to high concentrations of ethanol. High OsMDHAR expression also improved the fermentative capacity of the yeast during glucose-based batch fermentation at a standard cultivation temperature (30°C). The alcohol yield of OsMDHAR-expressing transgenic yeast during fermentation was approximately 25% (0.18 g·g-1) higher than that of WT yeast. Accordingly, OsMDHAR-expressing transgenic yeast showed prolonged survival during the environmental stresses produced during fermentation. These results suggest that heterologous OsMDHAR expression increases tolerance to reactive oxygen species-induced oxidative stress by improving cellular redox homeostasis and improves survival during fermentation, which enhances fermentative capacity. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/27392090/Potential_Application_of_the_Oryza_sativa_Monodehydroascorbate_Reductase_Gene__OsMDHAR__to_Improve_the_Stress_Tolerance_and_Fermentative_Capacity_of_Saccharomyces_cerevisiae_ L2 - https://dx.plos.org/10.1371/journal.pone.0158841 DB - PRIME DP - Unbound Medicine ER -