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Chemokine, cytokine and type I interferon production induced by Toll-like receptor activation in common variable immune deficiency.
Clin Immunol 2016; 169:121-127CI

Abstract

Common variable immunodeficiency (CVID) is the most common symptomatic primary antibody deficiency and is associated with recurrent infections and chronic inflammatory diseases. We evaluated the ability of Toll-like receptor (TLR) ligands to induce secretion of chemokines, cytokines and type I interferons by peripheral blood mononuclear cells (PBMCs) from CVID patients. High levels of CXCL10, CCL2, CXCL9, CCL5, CXCL8, and IL-6 were detected in sera of CVID patients compared with healthy controls. Increased chemokine levels were observed in unstimulated PBMCs, but after stimulation with TLR2 and TLR4 agonists, equivalent chemokine and pro-inflammatory cytokine secretion, as in healthy controls, was observed, whereas TLR4 agonist induced a decreased secretion of CCL2 and CXCL8 and increased secretion of TNF. Decreased IFN-α secretion induced by TLR7/TLR8 activation was observed in CVID, which was recovered with TLR9 signaling. Our findings revealed that TLR9 activation has an adjuvant effect on the altered type I response in CVID.

Authors+Show Affiliations

Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Medical School, University of São Paulo, São Paulo, Brazil.Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Medical School, University of São Paulo, São Paulo, Brazil.Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Medical School, University of São Paulo, São Paulo, Brazil.Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Medical School, University of São Paulo, São Paulo, Brazil.Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Medical School, University of São Paulo, São Paulo, Brazil.Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Medical School, University of São Paulo, São Paulo, Brazil.Laboratory of Dermatology and Immunodeficiencies, LIM-56, Department of Dermatology, Medical School, University of São Paulo, São Paulo, Brazil. Electronic address: marisato@usp.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27392462

Citation

Lollo, Camila de, et al. "Chemokine, Cytokine and Type I Interferon Production Induced By Toll-like Receptor Activation in Common Variable Immune Deficiency." Clinical Immunology (Orlando, Fla.), vol. 169, 2016, pp. 121-127.
Lollo C, de Moraes Vasconcelos D, Oliveira LMDS, et al. Chemokine, cytokine and type I interferon production induced by Toll-like receptor activation in common variable immune deficiency. Clin Immunol. 2016;169:121-127.
Lollo, C., de Moraes Vasconcelos, D., Oliveira, L. M. D. S., Domingues, R., Carvalho, G. C., Duarte, A. J. D. S., & Sato, M. N. (2016). Chemokine, cytokine and type I interferon production induced by Toll-like receptor activation in common variable immune deficiency. Clinical Immunology (Orlando, Fla.), 169, pp. 121-127. doi:10.1016/j.clim.2016.07.001.
Lollo C, et al. Chemokine, Cytokine and Type I Interferon Production Induced By Toll-like Receptor Activation in Common Variable Immune Deficiency. Clin Immunol. 2016;169:121-127. PubMed PMID: 27392462.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemokine, cytokine and type I interferon production induced by Toll-like receptor activation in common variable immune deficiency. AU - Lollo,Camila de, AU - de Moraes Vasconcelos,Dewton, AU - Oliveira,Luanda Mara da Silva, AU - Domingues,Rosana, AU - Carvalho,Gabriel Costa de, AU - Duarte,Alberto José da Silva, AU - Sato,Maria Notomi, Y1 - 2016/07/05/ PY - 2016/01/14/received PY - 2016/05/03/revised PY - 2016/07/03/accepted PY - 2016/7/10/entrez PY - 2016/7/10/pubmed PY - 2017/4/1/medline KW - Chemokines KW - Common variable immunodeficiency KW - Cytokines KW - Toll-like receptor agonists SP - 121 EP - 127 JF - Clinical immunology (Orlando, Fla.) JO - Clin. Immunol. VL - 169 N2 - Common variable immunodeficiency (CVID) is the most common symptomatic primary antibody deficiency and is associated with recurrent infections and chronic inflammatory diseases. We evaluated the ability of Toll-like receptor (TLR) ligands to induce secretion of chemokines, cytokines and type I interferons by peripheral blood mononuclear cells (PBMCs) from CVID patients. High levels of CXCL10, CCL2, CXCL9, CCL5, CXCL8, and IL-6 were detected in sera of CVID patients compared with healthy controls. Increased chemokine levels were observed in unstimulated PBMCs, but after stimulation with TLR2 and TLR4 agonists, equivalent chemokine and pro-inflammatory cytokine secretion, as in healthy controls, was observed, whereas TLR4 agonist induced a decreased secretion of CCL2 and CXCL8 and increased secretion of TNF. Decreased IFN-α secretion induced by TLR7/TLR8 activation was observed in CVID, which was recovered with TLR9 signaling. Our findings revealed that TLR9 activation has an adjuvant effect on the altered type I response in CVID. SN - 1521-7035 UR - https://www.unboundmedicine.com/medline/citation/27392462/Chemokine_cytokine_and_type_I_interferon_production_induced_by_Toll_like_receptor_activation_in_common_variable_immune_deficiency_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1521-6616(16)30148-6 DB - PRIME DP - Unbound Medicine ER -