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In vivo bactericidal effect of Melaleuca alternifolia essential oil against Aeromonas hydrophila: Silver catfish (Rhamdia quelen) as an experimental model.
Microb Pathog 2016; 98:82-7MP

Abstract

Aeromonas hydrophila is one of the main causative agent of high mortality and significative economic losses in aquaculture and has become increasingly resistant to conventional antibiotics. One feasible alternative to control and treat it is the use of essential oils. This study aimed to evaluate A. hydrophila susceptibility to tea tree oil (TTO-Melaleuca alternifolia) in vivo, and the effect of this treatment. In vivo tests were performed using silver catfish (Rhamdia quelen) as the experimental model. Silver catfish were treated with TTO at 25 and 50 μL/L for seven days before infection. After seven days, the fish were inoculated with A. hydrophila via intramuscularly. Treatment with TTO at 50 μL/L was able to extend longevity of infected fish, and showed 88% of therapeutic success, even though it did not show curative efficacy. TTO treatment was not toxic under these tested concentrations, since biomarkers of hepatic and renal functions were not affected, and the concentration of 50 μL/L was able to prevent increased levels of aspartate aminotransferase. There was no significative differences regarding hematological parameters (p < 0.05). Treatment with TTO 50 μL/L was able to reduce histopathological alterations usually caused by this type of bacteria in the gills, but it was unable to reduce hepatic histopathological alterations. Our results showed, for the first time, that TTO has high activity against A. hydrophila and proved to be a natural alternative to prevent and control this pathogen.

Authors+Show Affiliations

Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: carinedefs@yahoo.com.br.Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.Laboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, Brazil; Laboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.Laboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, Brazil; Laboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.Laboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.Laboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.Laboratory of Nanotechnology, Centro Universitário Franciscano, Santa Maria, RS, Brazil; Laboratory of Microbiology, Centro Universitário Franciscano, Santa Maria, RS, Brazil.Laboratory of Experimental Morphophysiology, Department of Morphology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.Laboratory of Experimental Morphophysiology, Department of Morphology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27392700

Citation

F Souza, Carine, et al. "In Vivo Bactericidal Effect of Melaleuca Alternifolia Essential Oil Against Aeromonas Hydrophila: Silver Catfish (Rhamdia Quelen) as an Experimental Model." Microbial Pathogenesis, vol. 98, 2016, pp. 82-7.
F Souza C, Baldissera MD, A Vaucher R, et al. In vivo bactericidal effect of Melaleuca alternifolia essential oil against Aeromonas hydrophila: Silver catfish (Rhamdia quelen) as an experimental model. Microb Pathog. 2016;98:82-7.
F Souza, C., Baldissera, M. D., A Vaucher, R., Lopes, L. Q., Vizzotto, B. S., Raffin, R. P., ... Baldisserotto, B. (2016). In vivo bactericidal effect of Melaleuca alternifolia essential oil against Aeromonas hydrophila: Silver catfish (Rhamdia quelen) as an experimental model. Microbial Pathogenesis, 98, pp. 82-7. doi:10.1016/j.micpath.2016.07.002.
F Souza C, et al. In Vivo Bactericidal Effect of Melaleuca Alternifolia Essential Oil Against Aeromonas Hydrophila: Silver Catfish (Rhamdia Quelen) as an Experimental Model. Microb Pathog. 2016;98:82-7. PubMed PMID: 27392700.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo bactericidal effect of Melaleuca alternifolia essential oil against Aeromonas hydrophila: Silver catfish (Rhamdia quelen) as an experimental model. AU - F Souza,Carine, AU - Baldissera,Matheus D, AU - A Vaucher,Rodrigo, AU - Lopes,Leonardo Q S, AU - Vizzotto,Bruno S, AU - Raffin,Renata P, AU - Santos,Roberto C V, AU - L da Veiga,Marcelo, AU - U M da Rocha,Maria Izabel, AU - Stefani,Lenita M, AU - Baldisserotto,Bernardo, Y1 - 2016/07/05/ PY - 2016/06/04/received PY - 2016/07/03/revised PY - 2016/07/04/accepted PY - 2016/7/10/entrez PY - 2016/7/10/pubmed PY - 2017/3/31/medline KW - Aeromonas hydrophila KW - Antimicrobial action KW - Fish KW - TTO SP - 82 EP - 7 JF - Microbial pathogenesis JO - Microb. Pathog. VL - 98 N2 - Aeromonas hydrophila is one of the main causative agent of high mortality and significative economic losses in aquaculture and has become increasingly resistant to conventional antibiotics. One feasible alternative to control and treat it is the use of essential oils. This study aimed to evaluate A. hydrophila susceptibility to tea tree oil (TTO-Melaleuca alternifolia) in vivo, and the effect of this treatment. In vivo tests were performed using silver catfish (Rhamdia quelen) as the experimental model. Silver catfish were treated with TTO at 25 and 50 μL/L for seven days before infection. After seven days, the fish were inoculated with A. hydrophila via intramuscularly. Treatment with TTO at 50 μL/L was able to extend longevity of infected fish, and showed 88% of therapeutic success, even though it did not show curative efficacy. TTO treatment was not toxic under these tested concentrations, since biomarkers of hepatic and renal functions were not affected, and the concentration of 50 μL/L was able to prevent increased levels of aspartate aminotransferase. There was no significative differences regarding hematological parameters (p < 0.05). Treatment with TTO 50 μL/L was able to reduce histopathological alterations usually caused by this type of bacteria in the gills, but it was unable to reduce hepatic histopathological alterations. Our results showed, for the first time, that TTO has high activity against A. hydrophila and proved to be a natural alternative to prevent and control this pathogen. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/27392700/In vivo_bactericidal_effect_of_Melaleuca_alternifolia_essential_oil_against_Aeromonas_hydrophila:_Silver_catfish__Rhamdia_quelen__as_an_experimental_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(16)30298-4 DB - PRIME DP - Unbound Medicine ER -