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Protective effect of gentiopicroside against dextran sodium sulfate induced colitis in mice.
Int Immunopharmacol 2016; 39:16-22II

Abstract

This study was designed to investigate the anti-inflammatory activity of the pure compound gentiopicroside (Gent) on dextran sulfate sodium (DSS)-induced colitis in a mouse model and to explore the possible related mechanisms. Experimental colitis was induced in ICR mice by dissolving 5% DSS in their drinking water for 7days. Gent (200, 100, and 50mg/kg) and 5-aminosalicylic acid (5-ASA, 100mg/kg) were oral administrated once a day for 7days. Anti-inflammatory effects were evaluated by comparing extend of colonic mucosal injury assessed by disease activity index (DAI), colon length, histopathological examination, and biochemical test. The possible mechanisms of Gent activities were explored by evaluating expression levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 using real-time fluorogenic PCR and expression levels of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) using Western blotting. The results showed that oral administration of Gent significantly attenuated DSS-induced loss of body weight, diarrhea, shortening of colon length and histological changes, associated with the decrease in the activity of myeloperoxidase (MPO) in the colon. In addition, the mRNA expression of TNF-α, IL-1β, IL-6 and the overexpression of COX-2 and iNOS proteins in the colon were down-regulated by Gent treatment. To our knowledge, this is the first study to demonstrate that Gent treatment can exert anti-inflammatory effects on experimental acute colitis through attenuating the expression levels of TNF-α, IL-1β, IL-6, iNOS and COX-2, and it may present the therapeutic potential in the treatment of colitis.

Authors+Show Affiliations

Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, China.Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, China.Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, China.Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, China.Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, China.Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, China.Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China.Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan 750004, China.College of Nursing, Ningxia Medical University, Yinchuan 750004, China. Electronic address: Li_yuxiang@163.com.Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, China; Ningxia Hui Medicine Modern Engineering Research Center, Ningxia Medical University, Yinchuan 750004, China. Electronic address: yujq910315@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27394986

Citation

Niu, Ya-Ting, et al. "Protective Effect of Gentiopicroside Against Dextran Sodium Sulfate Induced Colitis in Mice." International Immunopharmacology, vol. 39, 2016, pp. 16-22.
Niu YT, Zhao YP, Jiao YF, et al. Protective effect of gentiopicroside against dextran sodium sulfate induced colitis in mice. Int Immunopharmacol. 2016;39:16-22.
Niu, Y. T., Zhao, Y. P., Jiao, Y. F., Zheng, J., Yang, W. L., Zhou, R., ... Yu, J. Q. (2016). Protective effect of gentiopicroside against dextran sodium sulfate induced colitis in mice. International Immunopharmacology, 39, pp. 16-22. doi:10.1016/j.intimp.2016.07.003.
Niu YT, et al. Protective Effect of Gentiopicroside Against Dextran Sodium Sulfate Induced Colitis in Mice. Int Immunopharmacol. 2016;39:16-22. PubMed PMID: 27394986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of gentiopicroside against dextran sodium sulfate induced colitis in mice. AU - Niu,Ya-Ting, AU - Zhao,Yu-Ping, AU - Jiao,Yan-Fang, AU - Zheng,Jie, AU - Yang,Wen-Li, AU - Zhou,Ru, AU - Niu,Yang, AU - Sun,Tao, AU - Li,Yu-Xiang, AU - Yu,Jian-Qiang, Y1 - 2016/07/07/ PY - 2016/05/01/received PY - 2016/06/14/revised PY - 2016/07/04/accepted PY - 2016/7/11/entrez PY - 2016/7/11/pubmed PY - 2017/4/8/medline KW - Anti-inflammatory activity KW - Cyclooxygenase-2 KW - Gentiopicroside KW - Inducible nitric oxide synthase KW - Pro-inflammatory cytokines SP - 16 EP - 22 JF - International immunopharmacology JO - Int. Immunopharmacol. VL - 39 N2 - This study was designed to investigate the anti-inflammatory activity of the pure compound gentiopicroside (Gent) on dextran sulfate sodium (DSS)-induced colitis in a mouse model and to explore the possible related mechanisms. Experimental colitis was induced in ICR mice by dissolving 5% DSS in their drinking water for 7days. Gent (200, 100, and 50mg/kg) and 5-aminosalicylic acid (5-ASA, 100mg/kg) were oral administrated once a day for 7days. Anti-inflammatory effects were evaluated by comparing extend of colonic mucosal injury assessed by disease activity index (DAI), colon length, histopathological examination, and biochemical test. The possible mechanisms of Gent activities were explored by evaluating expression levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 using real-time fluorogenic PCR and expression levels of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) using Western blotting. The results showed that oral administration of Gent significantly attenuated DSS-induced loss of body weight, diarrhea, shortening of colon length and histological changes, associated with the decrease in the activity of myeloperoxidase (MPO) in the colon. In addition, the mRNA expression of TNF-α, IL-1β, IL-6 and the overexpression of COX-2 and iNOS proteins in the colon were down-regulated by Gent treatment. To our knowledge, this is the first study to demonstrate that Gent treatment can exert anti-inflammatory effects on experimental acute colitis through attenuating the expression levels of TNF-α, IL-1β, IL-6, iNOS and COX-2, and it may present the therapeutic potential in the treatment of colitis. SN - 1878-1705 UR - https://www.unboundmedicine.com/medline/citation/27394986/Protective_effect_of_gentiopicroside_against_dextran_sodium_sulfate_induced_colitis_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(16)30272-7 DB - PRIME DP - Unbound Medicine ER -