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Piper umbellatum L.: A medicinal plant with gastric-ulcer protective and ulcer healing effects in experimental rodent models.
J Ethnopharmacol. 2016 Nov 04; 192:123-131.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Piper umbellatum L. (Piperaceae) is a shrub found in the Amazon, Savannah and Atlantic Forest region of Brazil. It is widely used in folk medicine in many countries primarily for the treatment of gastric disorders. The aim of this study was to evaluate the gastroprotective and anti-ulcer effects of hydroethanolic extract of P. umbellatum (HEPu) leaves in experimental rodents. In addition, the anti-Helicobacter pylori activity of the extract was assessed.

MATERIALS AND METHODS

The leaves of P. umbellatum were macerated in 75% (1:3w/v) hydroethanolic solution to obtain HEPu. The gastroprotective and ulcer healing activities of HEPu were evaluated using acidified ethanol (acute) and acetic acid (chronic) gastric ulcer models in rodents. The anti-H. pylori activity was evaluated by in vitro broth microdilution assay using H. pylori cagA+ and vacA+ strain. The probable mechanism of action of HEPu was evaluated by determining gastric secretory parameters, antioxidant enzyme (catalase), non-protein sulfhydryl (glutathione) and malondialdehyde levels in gastric tissue, including pro-inflammatory (IL-1β, TNF-a, IL -17, RANTES, IFN-γ and MIP-2) and anti-inflammatory (IL-10) cytokines.

RESULTS

HEPu demonstrated potent gastroprotection against acute ulcer induced by acidified ethanol and excellent healing effect of the chronic ulcer induced by acetic acid. The gastroprotective activity in acidified ethanol is partly attributed to the antioxidant mechanisms, while anti-secretory, anti-inflammatory and regeneration of the gastric mucosa are evoked as part of its antiulcer mechanism of action. The gastric ulcer healing of HEPu also involves restoration of the altered cytokines levels to near normal. However, it has no in vitro anti-H. pylori activity.

CONCLUSION

The results of this study showed that HEPu possesses preventive and curative effects in experimental models of gastric ulcers in animals. These effects are partially dependent on antioxidant, antisecretory, anti-inflammatory and mucosa regeneration. It is independent of anti-H. pylori activity, with substances probably responsible for the pharmacological activity being flavonoids, quercetin and rutin. These results support the popular use of P. umbellatum leaves in the treatment of peptic ulcers.

Authors+Show Affiliations

Postgraduate Studies in Health Sciences, Faculty of Medicine, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.Postgraduate Studies in Health Sciences, Faculty of Medicine, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.Postgraduate Studies in Health Sciences, Faculty of Medicine, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.Department of Basic Health Sciences, Faculty of Medicine, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.Department of Basic Health Sciences, Faculty of Medicine, Universidade Federal de Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil. Electronic address: taba@terra.com.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27396348

Citation

da Silva Junior, Iberê Ferreira, et al. "Piper Umbellatum L.: a Medicinal Plant With Gastric-ulcer Protective and Ulcer Healing Effects in Experimental Rodent Models." Journal of Ethnopharmacology, vol. 192, 2016, pp. 123-131.
da Silva Junior IF, Balogun SO, de Oliveira RG, et al. Piper umbellatum L.: A medicinal plant with gastric-ulcer protective and ulcer healing effects in experimental rodent models. J Ethnopharmacol. 2016;192:123-131.
da Silva Junior, I. F., Balogun, S. O., de Oliveira, R. G., Damazo, A. S., & Martins, D. T. O. (2016). Piper umbellatum L.: A medicinal plant with gastric-ulcer protective and ulcer healing effects in experimental rodent models. Journal of Ethnopharmacology, 192, 123-131. https://doi.org/10.1016/j.jep.2016.07.011
da Silva Junior IF, et al. Piper Umbellatum L.: a Medicinal Plant With Gastric-ulcer Protective and Ulcer Healing Effects in Experimental Rodent Models. J Ethnopharmacol. 2016 Nov 4;192:123-131. PubMed PMID: 27396348.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Piper umbellatum L.: A medicinal plant with gastric-ulcer protective and ulcer healing effects in experimental rodent models. AU - da Silva Junior,Iberê Ferreira, AU - Balogun,Sikiru Olaitan, AU - de Oliveira,Ruberlei Godinho, AU - Damazo,Amílcar Sabino, AU - Martins,Domingos Tabajara de Oliveira, Y1 - 2016/07/07/ PY - 2016/05/03/received PY - 2016/07/04/revised PY - 2016/07/07/accepted PY - 2016/7/12/pubmed PY - 2017/4/22/medline PY - 2016/7/12/entrez KW - Anti-ulcer KW - Cytokines KW - Flavonoids KW - Mechanism of action KW - Piper umbellatum SP - 123 EP - 131 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 192 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Piper umbellatum L. (Piperaceae) is a shrub found in the Amazon, Savannah and Atlantic Forest region of Brazil. It is widely used in folk medicine in many countries primarily for the treatment of gastric disorders. The aim of this study was to evaluate the gastroprotective and anti-ulcer effects of hydroethanolic extract of P. umbellatum (HEPu) leaves in experimental rodents. In addition, the anti-Helicobacter pylori activity of the extract was assessed. MATERIALS AND METHODS: The leaves of P. umbellatum were macerated in 75% (1:3w/v) hydroethanolic solution to obtain HEPu. The gastroprotective and ulcer healing activities of HEPu were evaluated using acidified ethanol (acute) and acetic acid (chronic) gastric ulcer models in rodents. The anti-H. pylori activity was evaluated by in vitro broth microdilution assay using H. pylori cagA+ and vacA+ strain. The probable mechanism of action of HEPu was evaluated by determining gastric secretory parameters, antioxidant enzyme (catalase), non-protein sulfhydryl (glutathione) and malondialdehyde levels in gastric tissue, including pro-inflammatory (IL-1β, TNF-a, IL -17, RANTES, IFN-γ and MIP-2) and anti-inflammatory (IL-10) cytokines. RESULTS: HEPu demonstrated potent gastroprotection against acute ulcer induced by acidified ethanol and excellent healing effect of the chronic ulcer induced by acetic acid. The gastroprotective activity in acidified ethanol is partly attributed to the antioxidant mechanisms, while anti-secretory, anti-inflammatory and regeneration of the gastric mucosa are evoked as part of its antiulcer mechanism of action. The gastric ulcer healing of HEPu also involves restoration of the altered cytokines levels to near normal. However, it has no in vitro anti-H. pylori activity. CONCLUSION: The results of this study showed that HEPu possesses preventive and curative effects in experimental models of gastric ulcers in animals. These effects are partially dependent on antioxidant, antisecretory, anti-inflammatory and mucosa regeneration. It is independent of anti-H. pylori activity, with substances probably responsible for the pharmacological activity being flavonoids, quercetin and rutin. These results support the popular use of P. umbellatum leaves in the treatment of peptic ulcers. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/27396348/Piper_umbellatum_L_:_A_medicinal_plant_with_gastric_ulcer_protective_and_ulcer_healing_effects_in_experimental_rodent_models_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)30435-4 DB - PRIME DP - Unbound Medicine ER -