Tags

Type your tag names separated by a space and hit enter

Panax notoginseng saponins ameliorates experimental hepatic fibrosis and hepatic stellate cell proliferation by inhibiting the Jak2/ Stat3 pathways.
J Tradit Chin Med. 2016 Apr; 36(2):217-24.JT

Abstract

OBJECTIVE

To investigate the inhibitory effect of Panax notoginseng saponins (PNS) on liver fibrosis and explore the underlying mechanisms.

METHODS

Carbon tetrachloride (CCl4)-treated rats and hepatic stellate cells (HSCs) were used. The effect of PNS on CCl4-induced liver fibrosis was studied with histochemical and biochemical analysis. Transforming growth factor (TGF)-β1, α-smooth muscle actin (α-SMA), and collagen I mRNA expression were determined by reverse transcripwhile, the protein expression levels of α-SMA, collagen I, phosphorylation-Janus activated kinase signal transducer (p-Jak2)/Jak2, and phosphorylation-activator of transcription (p-Stat)3/Stat3 were determined by immunohistochemistry and/or immunoblotting.

RESULTS

PNS treatment significantly improved the liver function of rats as indicated by decreased serum enzymatic activities of alanine aminotransferase and aspartate aminotransferase. Histopathological results indicated that PNS alleviated liver damage and reduced the formation of fibrous septa. Moreover, PNS significantly decreased liver hydroxyproline and significantly attenuated expressions of collagen I, α-SMA, TGF-β1, p-Jak2 / Jak2, and p-Stat3/Stat3 in the rat liver fibrosis model and HSCs.

CONCLUSION

PNS can relieve liver fibrosis by modulating Jak2/Stat3 signaling transduction pathway, which may be one of its mechanisms to suppress hepatic fibrosis.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27400477

Citation

Hui, Jiang, et al. "Panax Notoginseng Saponins Ameliorates Experimental Hepatic Fibrosis and Hepatic Stellate Cell Proliferation By Inhibiting the Jak2/ Stat3 Pathways." Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan, vol. 36, no. 2, 2016, pp. 217-24.
Hui J, Gao J, Wang Y, et al. Panax notoginseng saponins ameliorates experimental hepatic fibrosis and hepatic stellate cell proliferation by inhibiting the Jak2/ Stat3 pathways. J Tradit Chin Med. 2016;36(2):217-24.
Hui, J., Gao, J., Wang, Y., Zhang, J., Han, Y., Wei, L., Liu Xiaochuang, ., & Wu, J. (2016). Panax notoginseng saponins ameliorates experimental hepatic fibrosis and hepatic stellate cell proliferation by inhibiting the Jak2/ Stat3 pathways. Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan, 36(2), 217-24.
Hui J, et al. Panax Notoginseng Saponins Ameliorates Experimental Hepatic Fibrosis and Hepatic Stellate Cell Proliferation By Inhibiting the Jak2/ Stat3 Pathways. J Tradit Chin Med. 2016;36(2):217-24. PubMed PMID: 27400477.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Panax notoginseng saponins ameliorates experimental hepatic fibrosis and hepatic stellate cell proliferation by inhibiting the Jak2/ Stat3 pathways. AU - Hui,Jiang, AU - Gao,Jiarong, AU - Wang,Yongzhong, AU - Zhang,Jiafu, AU - Han,Yanquan, AU - Wei,Liangbing, AU - Liu Xiaochuang,, AU - Wu,Jian, PY - 2016/7/13/entrez PY - 2016/7/13/pubmed PY - 2016/9/23/medline SP - 217 EP - 24 JF - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan JO - J Tradit Chin Med VL - 36 IS - 2 N2 - OBJECTIVE: To investigate the inhibitory effect of Panax notoginseng saponins (PNS) on liver fibrosis and explore the underlying mechanisms. METHODS: Carbon tetrachloride (CCl4)-treated rats and hepatic stellate cells (HSCs) were used. The effect of PNS on CCl4-induced liver fibrosis was studied with histochemical and biochemical analysis. Transforming growth factor (TGF)-β1, α-smooth muscle actin (α-SMA), and collagen I mRNA expression were determined by reverse transcripwhile, the protein expression levels of α-SMA, collagen I, phosphorylation-Janus activated kinase signal transducer (p-Jak2)/Jak2, and phosphorylation-activator of transcription (p-Stat)3/Stat3 were determined by immunohistochemistry and/or immunoblotting. RESULTS: PNS treatment significantly improved the liver function of rats as indicated by decreased serum enzymatic activities of alanine aminotransferase and aspartate aminotransferase. Histopathological results indicated that PNS alleviated liver damage and reduced the formation of fibrous septa. Moreover, PNS significantly decreased liver hydroxyproline and significantly attenuated expressions of collagen I, α-SMA, TGF-β1, p-Jak2 / Jak2, and p-Stat3/Stat3 in the rat liver fibrosis model and HSCs. CONCLUSION: PNS can relieve liver fibrosis by modulating Jak2/Stat3 signaling transduction pathway, which may be one of its mechanisms to suppress hepatic fibrosis. SN - 0255-2922 UR - https://www.unboundmedicine.com/medline/citation/27400477/Panax_notoginseng_saponins_ameliorates_experimental_hepatic_fibrosis_and_hepatic_stellate_cell_proliferation_by_inhibiting_the_Jak2/_Stat3_pathways_ L2 - http://www.journaltcm.com/index.php?module=Journal&func=downloadFullContent&sid=2522 DB - PRIME DP - Unbound Medicine ER -