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Antimicrobial and anti-Quorum Sensing activities of selected medicinal plants of Ethiopia: Implication for development of potent antimicrobial agents.
BMC Microbiol. 2016 07 11; 16(1):139.BM

Abstract

BACKGROUND

Traditional medicinal plants have been used as an alternative medicine in many parts of the world, including Ethiopia. There are many documented scientific reports on antimicrobial activities of the same. To our knowledge, however, there is no report on the anti-Quorum Sensing (Quorum Quenching, QQ) potential of traditional Ethiopian medicinal plants. As many of the opportunistic pathogenic bacteria depend on Quorum Sensing (QS) systems to coordinate their virulence expression, interference with QS could be a novel approach to control bacterial infections. Thus, the aim of this study was to evaluate selected medicinal plants from Ethiopia for their antimicrobial activities against bacterial and fungal pathogens; and to assess the interference of these plant extracts with QS of bacteria.

METHODS

Antimicrobial activities of plant extracts (oil, resins and crude extracts) were evaluated following standard agar diffusion technique. The minimum inhibitory concentrations (MIC) of potent extracts were determined using 96 well micro-titer plates and optical densities were measured using an ELISA Microplate reader. Interference with Quorum Sensing activities of extracts was determined using the recently established E. coli based reporter strain AI1-QQ.1 and signaling molecule N-(β-ketocaproyl)-L-homoserine lactone (3-oxo-C6-HSL).

RESULTS

Petroleum ether extract of seed of Nigella sativa exhibited the highest activity against both the laboratory isolated Bacillus cereus [inhibition zone (IZ), 44 ± 0.31 mm] and B. cereus ATCC 10987 (IZ, 40 ± 2.33 mm). Similarly, oil extract from mature ripe fruit husk of Aframomum corrorima and mature unripe fruit of A. corrorima revealed promising activities against Candida albicans ATCC 90028 (IZ, 35 ± 1.52 mm) and Staphylococcus aureus DSM 346 (IZ, 25 ± 1.32 mm), respectively. Antimicrobial activities of oil extract from husk of A. corrorima and petroleum ether extract of seed of N. sativa were significantly higher than that of the control antibiotic [Gentamycin sulfate, (IZ, 25-30 mm)]. The lowest MIC value (12.5 mg/mL) was recorded for oil from husk of A. corrorima against Pseudomonas aeruginosa. Of the total eighteen extracts evaluated, two of the extracts [Methanol extract of root of Albiza schimperiana (ASRM) and petroleum ether extract of seed of Justica schimperiana (JSSP)] interfered with cell-cell communication most likely by interacting with the signaling molecules.

CONCLUSION

Traditional medicinal plants from Ethiopia are potential source of alternative medicine for the local community and scientific research in search for alternative drugs to halt challenges associated with the emerging antimicrobial resistance. Furthermore, the Quorum Quenching activities observed in two of the plant extracts calls for more comprehensive evaluation of medicinal plants for the control of many bacterial processes and phenotypic behaviors such as pathogenicity, swarming, and biofilm formation. Being the first assessment of its kind on the potential application of Ethiopian traditional medicinal plants for interference in microbial cell-cell communication (anti-Quorum Sensing activities), the detailed chemistry of the active compounds and possible mechanism(s) of actions of the bio-molecules responsible for the observed interference were not addressed in the current study. Thus, further evaluation for the nature of those active compounds (bio-molecules) and detailed mechanism(s) of their interaction with microbial processes are recommended.

Authors+Show Affiliations

Depatment of Biology, College of Natural Sciences, Jimma University, Jimma, Ethiopia. ketemabacha2002@yahoo.com.Department of Chemistry, College of Natural Sciences, Jimma University, Jimma, Ethiopia.Department of Horticulture, College of Agriculture, Adigrat University, Adigrat, Ethiopia.Department of Horticulture and Plant Science, College of Agriculture and Natural Resources Management, Gambella University, Gambella, Ethiopia.Departemnt of Postharvest Management, College of Agriculture and Veterinary Medicine, Jimma University, Jimma, Ethiopia.Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany.Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany.Depatment of Biology, College of Natural Sciences, Jimma University, Jimma, Ethiopia. Derpartment of Biology, College of Natural and Computational Sciences, Wollo University, Dessie, Ethiopia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27400878

Citation

Bacha, Ketema, et al. "Antimicrobial and anti-Quorum Sensing Activities of Selected Medicinal Plants of Ethiopia: Implication for Development of Potent Antimicrobial Agents." BMC Microbiology, vol. 16, no. 1, 2016, p. 139.
Bacha K, Tariku Y, Gebreyesus F, et al. Antimicrobial and anti-Quorum Sensing activities of selected medicinal plants of Ethiopia: Implication for development of potent antimicrobial agents. BMC Microbiol. 2016;16(1):139.
Bacha, K., Tariku, Y., Gebreyesus, F., Zerihun, S., Mohammed, A., Weiland-Bräuer, N., Schmitz, R. A., & Mulat, M. (2016). Antimicrobial and anti-Quorum Sensing activities of selected medicinal plants of Ethiopia: Implication for development of potent antimicrobial agents. BMC Microbiology, 16(1), 139. https://doi.org/10.1186/s12866-016-0765-9
Bacha K, et al. Antimicrobial and anti-Quorum Sensing Activities of Selected Medicinal Plants of Ethiopia: Implication for Development of Potent Antimicrobial Agents. BMC Microbiol. 2016 07 11;16(1):139. PubMed PMID: 27400878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial and anti-Quorum Sensing activities of selected medicinal plants of Ethiopia: Implication for development of potent antimicrobial agents. AU - Bacha,Ketema, AU - Tariku,Yinebeb, AU - Gebreyesus,Fisseha, AU - Zerihun,Shibru, AU - Mohammed,Ali, AU - Weiland-Bräuer,Nancy, AU - Schmitz,Ruth A, AU - Mulat,Mulugeta, Y1 - 2016/07/11/ PY - 2016/02/03/received PY - 2016/07/07/accepted PY - 2016/7/13/entrez PY - 2016/7/13/pubmed PY - 2017/6/9/medline KW - Alternative medicine KW - Drug resistance KW - Ethiopia KW - MIC KW - Medicinal plants KW - Quorum Quenching KW - Quorum Sensing SP - 139 EP - 139 JF - BMC microbiology JO - BMC Microbiol VL - 16 IS - 1 N2 - BACKGROUND: Traditional medicinal plants have been used as an alternative medicine in many parts of the world, including Ethiopia. There are many documented scientific reports on antimicrobial activities of the same. To our knowledge, however, there is no report on the anti-Quorum Sensing (Quorum Quenching, QQ) potential of traditional Ethiopian medicinal plants. As many of the opportunistic pathogenic bacteria depend on Quorum Sensing (QS) systems to coordinate their virulence expression, interference with QS could be a novel approach to control bacterial infections. Thus, the aim of this study was to evaluate selected medicinal plants from Ethiopia for their antimicrobial activities against bacterial and fungal pathogens; and to assess the interference of these plant extracts with QS of bacteria. METHODS: Antimicrobial activities of plant extracts (oil, resins and crude extracts) were evaluated following standard agar diffusion technique. The minimum inhibitory concentrations (MIC) of potent extracts were determined using 96 well micro-titer plates and optical densities were measured using an ELISA Microplate reader. Interference with Quorum Sensing activities of extracts was determined using the recently established E. coli based reporter strain AI1-QQ.1 and signaling molecule N-(β-ketocaproyl)-L-homoserine lactone (3-oxo-C6-HSL). RESULTS: Petroleum ether extract of seed of Nigella sativa exhibited the highest activity against both the laboratory isolated Bacillus cereus [inhibition zone (IZ), 44 ± 0.31 mm] and B. cereus ATCC 10987 (IZ, 40 ± 2.33 mm). Similarly, oil extract from mature ripe fruit husk of Aframomum corrorima and mature unripe fruit of A. corrorima revealed promising activities against Candida albicans ATCC 90028 (IZ, 35 ± 1.52 mm) and Staphylococcus aureus DSM 346 (IZ, 25 ± 1.32 mm), respectively. Antimicrobial activities of oil extract from husk of A. corrorima and petroleum ether extract of seed of N. sativa were significantly higher than that of the control antibiotic [Gentamycin sulfate, (IZ, 25-30 mm)]. The lowest MIC value (12.5 mg/mL) was recorded for oil from husk of A. corrorima against Pseudomonas aeruginosa. Of the total eighteen extracts evaluated, two of the extracts [Methanol extract of root of Albiza schimperiana (ASRM) and petroleum ether extract of seed of Justica schimperiana (JSSP)] interfered with cell-cell communication most likely by interacting with the signaling molecules. CONCLUSION: Traditional medicinal plants from Ethiopia are potential source of alternative medicine for the local community and scientific research in search for alternative drugs to halt challenges associated with the emerging antimicrobial resistance. Furthermore, the Quorum Quenching activities observed in two of the plant extracts calls for more comprehensive evaluation of medicinal plants for the control of many bacterial processes and phenotypic behaviors such as pathogenicity, swarming, and biofilm formation. Being the first assessment of its kind on the potential application of Ethiopian traditional medicinal plants for interference in microbial cell-cell communication (anti-Quorum Sensing activities), the detailed chemistry of the active compounds and possible mechanism(s) of actions of the bio-molecules responsible for the observed interference were not addressed in the current study. Thus, further evaluation for the nature of those active compounds (bio-molecules) and detailed mechanism(s) of their interaction with microbial processes are recommended. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/27400878/Antimicrobial_and_anti_Quorum_Sensing_activities_of_selected_medicinal_plants_of_Ethiopia:_Implication_for_development_of_potent_antimicrobial_agents_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0765-9 DB - PRIME DP - Unbound Medicine ER -