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ELISA Detection of 30 New Amphetamine Designer Drugs in Whole Blood, Urine and Oral Fluid using Neogen® "Amphetamine" and "Methamphetamine/MDMA" Kits.
J Anal Toxicol 2016; 40(7):492-7JA

Abstract

Amphetamine designer drugs are central nervous system stimulants that are widely disseminated in the illegal market. Generally, in forensic laboratories, immunoassay methods are the first line of screening for these types of drugs in a biological specimen (typically blood, urine or oral fluid). In this article, we describe the cross-reactivity profiles of 30 new amphetamine designer drugs, using the Neogen(®) [Amphetamine Specific and Methamphetamine/3,4-Methylenedioxymethamphetamine (MDMA) assays] drug tests. To assess the potential matrix influence on the response, each assay was tested on whole blood, urine and oral fluid. Concentrations of 10,000 ng/mL were not sufficient to produce a positive response for the majority of the analyzed amphetamines. This clearly demonstrates that, although these kits are extremely effective for the target drugs for which they are intended (amphetamine, methamphetamine and MDMA), they cannot be used to reliably identify the tested designer drugs in real cases, as these concentrations greatly exceed those expected to be found in forensic samples.

Authors+Show Affiliations

Dipartimento di Chimica e Farmacia, Università di Sassari, Via Muroni 23, 07100 Sassari, Italy.Dipartimento di Chimica e Farmacia, Università di Sassari, Via Muroni 23, 07100 Sassari, Italy lburrai@uniss.it.Dipartimento di Medicina Veterinaria, Università di Sassari, Via Vienna 2, 07100 Sassari, Italy.Dipartimento di Medicina Veterinaria, Università di Sassari, Via Vienna 2, 07100 Sassari, Italy.Dipartimento di Medicina Veterinaria, Università di Sassari, Via Vienna 2, 07100 Sassari, Italy.Dipartimento di Chimica e Farmacia, Università di Sassari, Via Muroni 23, 07100 Sassari, Italy.Dipartimento di Medicina Veterinaria, Università di Sassari, Via Vienna 2, 07100 Sassari, Italy.Dipartimento di Chimica e Farmacia, Università di Sassari, Via Muroni 23, 07100 Sassari, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27405364

Citation

Nieddu, Maria, et al. "ELISA Detection of 30 New Amphetamine Designer Drugs in Whole Blood, Urine and Oral Fluid Using Neogen® "Amphetamine" and "Methamphetamine/MDMA" Kits." Journal of Analytical Toxicology, vol. 40, no. 7, 2016, pp. 492-7.
Nieddu M, Burrai L, Baralla E, et al. ELISA Detection of 30 New Amphetamine Designer Drugs in Whole Blood, Urine and Oral Fluid using Neogen® "Amphetamine" and "Methamphetamine/MDMA" Kits. J Anal Toxicol. 2016;40(7):492-7.
Nieddu, M., Burrai, L., Baralla, E., Pasciu, V., Varoni, M. V., Briguglio, I., ... Boatto, G. (2016). ELISA Detection of 30 New Amphetamine Designer Drugs in Whole Blood, Urine and Oral Fluid using Neogen® "Amphetamine" and "Methamphetamine/MDMA" Kits. Journal of Analytical Toxicology, 40(7), pp. 492-7. doi:10.1093/jat/bkw060.
Nieddu M, et al. ELISA Detection of 30 New Amphetamine Designer Drugs in Whole Blood, Urine and Oral Fluid Using Neogen® "Amphetamine" and "Methamphetamine/MDMA" Kits. J Anal Toxicol. 2016;40(7):492-7. PubMed PMID: 27405364.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ELISA Detection of 30 New Amphetamine Designer Drugs in Whole Blood, Urine and Oral Fluid using Neogen® "Amphetamine" and "Methamphetamine/MDMA" Kits. AU - Nieddu,Maria, AU - Burrai,Lucia, AU - Baralla,Elena, AU - Pasciu,Valeria, AU - Varoni,Maria Vittoria, AU - Briguglio,Irene, AU - Demontis,Maria Piera, AU - Boatto,Gianpiero, Y1 - 2016/07/11/ PY - 2016/11/06/received PY - 2016/04/24/accepted PY - 2016/7/14/entrez PY - 2016/7/14/pubmed PY - 2018/1/13/medline SP - 492 EP - 7 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 40 IS - 7 N2 - Amphetamine designer drugs are central nervous system stimulants that are widely disseminated in the illegal market. Generally, in forensic laboratories, immunoassay methods are the first line of screening for these types of drugs in a biological specimen (typically blood, urine or oral fluid). In this article, we describe the cross-reactivity profiles of 30 new amphetamine designer drugs, using the Neogen(®) [Amphetamine Specific and Methamphetamine/3,4-Methylenedioxymethamphetamine (MDMA) assays] drug tests. To assess the potential matrix influence on the response, each assay was tested on whole blood, urine and oral fluid. Concentrations of 10,000 ng/mL were not sufficient to produce a positive response for the majority of the analyzed amphetamines. This clearly demonstrates that, although these kits are extremely effective for the target drugs for which they are intended (amphetamine, methamphetamine and MDMA), they cannot be used to reliably identify the tested designer drugs in real cases, as these concentrations greatly exceed those expected to be found in forensic samples. SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/27405364/ELISA_Detection_of_30_New_Amphetamine_Designer_Drugs_in_Whole_Blood_Urine_and_Oral_Fluid_using_Neogen®_"Amphetamine"_and_"Methamphetamine/MDMA"_Kits_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/bkw060 DB - PRIME DP - Unbound Medicine ER -